本文選題：新發(fā)展晚期血吸蟲病 + 基質(zhì)輔助激光解吸電離飛行時間質(zhì)譜��； 參考：《南京醫(yī)科大學(xué)學(xué)報(自然科學(xué)版)》2015年04期

【摘要】：目的 :分析新發(fā)展晚期血吸蟲病患者血清多肽,篩選有潛在應(yīng)用價值的標(biāo)志蛋白,為其診斷與發(fā)病機制研究奠定基礎(chǔ)。方法:采集新發(fā)展晚期血吸蟲病患者與健康人血清,利用包被有弱陽離子的磁珠富集純化血清多肽,應(yīng)用基質(zhì)輔助激光解析電離時間飛行質(zhì)譜(MALDI-TOF-MS)Flex分析技術(shù)結(jié)合Mascot生物信息學(xué)分析血清多肽指紋圖譜。結(jié)果:與健康對照組比較,在0.8~13.0 ku區(qū)段,新發(fā)展晚期血吸蟲病患者血清中m/z 2 082、4 282多肽表達(dá)上調(diào)(P0.01),2 661、2 991、3 241、5 337、5 906多肽表達(dá)下調(diào)(P0.05);常規(guī)晚期血吸蟲病患者血清中m/z 2 662、4 209、5 337與5 906處顯著下調(diào),2 082處顯著上調(diào)。結(jié)論:m/z3 241、4 282血清差異多肽對新發(fā)展晚期血吸蟲病患者的診斷可能具有潛在應(yīng)用價值,對該病發(fā)生發(fā)展的機制也奠定了理論基礎(chǔ)。
[Abstract]:Objective: to analyze the serum polypeptides of patients with advanced schistosomiasis and to screen potential marker proteins for the diagnosis and pathogenesis of schistosomiasis. Methods: serum samples of newly developed advanced schistosomiasis patients and healthy persons were collected and purified by magnetic beads coated with weak cations. Matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS Flex) was used to analyze serum polypeptide fingerprints with Mascot bioinformatics. Results: compared with the healthy control group, in the region of 0.8 ~ 13.0 ku, In the serum of newly developed advanced schistosomiasis patients, the expression of m / z 2 082n 4 282 polypeptide was up-regulated, and the expression of m- / z 2662 + 4209H 5 337 and 5 906 was significantly down-regulated at 2 082 and 2 082 points respectively in the serum of newly developed patients with advanced schistosomiasis, and the expression of P0.05906 polypeptide was down-regulated at 3241U 5337N 5906, and that in the serum of patients with advanced schistosomiasis was significantly lower than that in the patients with advanced schistosomiasis. ConclusionThe differential polypeptide in serum of w / m / z _ 3 241N _ 4 282 may have potential application value in the diagnosis of newly developed advanced schistosomiasis, and it also lays a theoretical foundation for the pathogenesis of schistosomiasis.
【作者單位】： 江蘇省血吸蟲病防治研究所衛(wèi)生部寄生蟲病預(yù)防與控制技術(shù)重點實驗室江蘇省寄生蟲分子生物學(xué)重點實驗室;常熟市疾病預(yù)防控制中心;
【基金】：國家自然科學(xué)基金項目(31201893) 江蘇省臨床醫(yī)學(xué)專項(BL2014020) 江蘇省自然科學(xué)基金項目(BK2011164) 江蘇省衛(wèi)生科研項目(X201104,X201110,X201416) 江蘇省臨床醫(yī)學(xué)中心項目(201108) 江蘇省動物預(yù)防醫(yī)學(xué)重點實驗室開放課題(K13045)
【分類號】：R532.21

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 仇志琴;黃玉政;陶永輝;張英;虞豐;;血清差異表達(dá)多肽譜用于人卵巢癌診斷的研究[J];南京醫(yī)科大學(xué)學(xué)報(自然科學(xué)版);2012年07期

2 黃玉政;徐明;王s，

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