發(fā)布時(shí)間：2018-05-07 14:34

  本文選題：人乳頭瘤病毒 + 男性　； 參考：《蚌埠醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:研究女性HPV感染者的男性性伴侶病毒HPV感染狀況及其外周血中淋巴細(xì)胞亞群、免疫球蛋白和補(bǔ)體含量變化,從而為阻斷HPV傳播及相關(guān)疾病的預(yù)防和免疫治療提供依據(jù)。方法:選擇110例女性HPV感染者的男性性伴侶作為研究對象,取其尿道口上皮細(xì)胞及空腹外周血。所有研究對象均需簽署知情同意書。采用PCR-反向點(diǎn)雜交法進(jìn)行HPV分型檢測,以流式細(xì)胞儀進(jìn)行淋巴細(xì)胞亞群檢測(CD3+、CD4+、CD8+、CD4+/CD8+、CD16+56+、CD19+細(xì)胞),以特種蛋白分析儀免疫散射比濁法檢測免疫球蛋白(IgA、IgG、IgM)及補(bǔ)體(C3、C4)含量。使用SPSS19.0版軟件對數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:1.女性單一亞型感染占61.82%(68/110),多重感染占38.18%(42/110)。110例女性HPV感染者的男性性伴侶的感染率為感染率33.64%(37/110),其中單一亞型感染占62.16%(23/37),多重感染占37.84%(14/37)。2.男女高低危HPV亞型出現(xiàn)頻次有顯著性統(tǒng)計(jì)學(xué)差異(X2=4.040,P=0.0440.05)。3.性伴雙方HPV吻合率為54.05%(20/37)。4.無癥狀男性HPV陰性組與陽性組外周血免疫指標(biāo)比較,差異無統(tǒng)計(jì)學(xué)意義(P均0.05)。5.HPV高危型組和低危型組CD8+T細(xì)胞、CD4+/CD8+T細(xì)胞比值均有顯著性統(tǒng)計(jì)學(xué)差異(P均0.05);多重高危型感染者外周血細(xì)胞免疫指標(biāo)變化較大;多重高危型HPV感染(1.50±0.42)較單一低危型感染(0.76±0.27)IgM含量有顯著性差異(P0.05)。6.免疫指標(biāo)正常組和異常組比較HPV感染率無統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論:1.該地區(qū)HPV感染的女性及其男性性伴,雙方HPV感染以單一亞型、高危亞型為主;女性對高危型HPV易感性高于男性,而男性對低危型HPV的易感性高于女性;HPV在性伴中傳播,控制男性HPV感染,減少HPV在性伴侶之間的循環(huán)感染,具有重要意義。2.高危型HPV無癥狀男性感染者外周血免疫功能主要趨向于細(xì)胞免疫抑制狀態(tài),多重高危型感染尤為明顯;血清IgM可能在HPV感染早期發(fā)揮重要作用。
[Abstract]:Objective: to study the status of male sexual chaperone virus (HPV) infection and the changes of lymphocyte subsets, immunoglobulin and complement levels in peripheral blood of female patients with HPV, so as to provide evidence for blocking the transmission of HPV and the prevention and immunotherapy of related diseases. Methods: the male sexual partners of 110 women with HPV infection were selected as the study subjects, and their urethral orifice epithelial cells and fasting peripheral blood were collected. All subjects are required to sign informed consent. HPV typing was carried out by using PCR- reverse dot hybridization, lymphocyte subsets were detected by flow cytometry (FCM), CD4 / CD8 / CD8 / CD1656 / CD19 cells were detected by flow cytometry, and the immunoglobulin IgA (IgA) and complement C _ (3) C _ (4) were detected by immunosurgery and turbidimetry by special protein analyzer. Use the SPSS19.0 version of the software for statistical analysis of the data. The result is 1: 1. Female single subtype infection accounted for 61.82% 68 / 110, multiple infection accounted for 38.18% 42 / 110% male sexual partner infection rate was 33.64% 37 / 110%, among them, single subtype infection accounted for 62.16% 23 / 37%, multiple heavy infection accounted for 37.84% 14 / 37.2. There was significant statistical difference in the frequency of HPV subtypes in male and female patients with high and low risk. The rate of HPV anastomosis was 54.05%, 20% 37%. 4%. The peripheral blood immunological indexes of asymptomatic male HPV negative group and positive group were compared. There was no significant difference in the ratio of CD4 / CD8 T cell between 0.05).5.HPV high risk group and low risk group. There was significant difference in the content of 0.76 鹵0.27)IgM between multiple high risk type HPV infection and single low risk type infection (P 0.05 鹵0.42). There was no significant difference in HPV infection rate between normal group and abnormal group (P 0.05). Conclusion 1. In this area, women with HPV infection and their male sexual partners had a single subtype and high risk subtype of HPV infection, while women were more susceptible to high risk HPV than men, and men were more susceptible to low risk HPV than women. It is important to control male HPV infection and reduce HPV circulating infection between sexual partners. The immune function of peripheral blood of high risk HPV asymptomatic male patients tended to be mainly cellular immunosuppressive, especially multiple high risk type infection, and serum IgM may play an important role in the early stage of HPV infection.
【學(xué)位授予單位】：蚌埠醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R511

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