本文選題：艾滋病病毒型 + 長期無進(jìn)展者　； 參考：《中國艾滋病性病》2016年09期

【摘要】：目的分析感染艾滋病病毒1型(HIV-1)后長期無進(jìn)展者體內(nèi)病毒的序列特征,探討影響艾滋病疾病進(jìn)程的病毒學(xué)因素。方法HIV-1病毒載量用HIV-1 Monitor Version 1.5版本試劑在AmplicorCobas上檢測;CD4+T淋巴細(xì)胞計數(shù)用multitest四色試劑在FASCalibur上進(jìn)行計數(shù);HIV-1近全長序列用三對引物擴(kuò)增后進(jìn)行測序,用ContigExpress軟件進(jìn)行序列編輯和拼接;HIV-1亞型用在線的REGA HIV-1Subtyping Tool-Version 3.0軟件分析;HIV-1的溯源用BEAST軟件包,采用貝葉斯馬爾可夫鏈-蒙特卡羅(MCMC)算法構(gòu)建系統(tǒng)進(jìn)化樹,推斷該HIV-1毒株在中國最初的流行時間和地區(qū);將HIV-1各蛋白的序列提交到至SWISS-MODEL蛋白質(zhì)同源建模數(shù)據(jù)庫(http://swissmodel.expasy.org/interactive),構(gòu)建蛋白質(zhì)結(jié)構(gòu)。結(jié)果該感染者在潛伏期體內(nèi)的病毒載量處于較低水平(4.32×103拷貝/mL),外周血中CD4+T淋巴細(xì)胞在感染10年后仍然高于500個/μL,該感染者感染的病毒為B亞型,最初于1992年左右在我國河南省流行。該病毒編碼的Vpu與參考株HBX2編碼的Vpu蛋白相比,結(jié)構(gòu)存在顯著的差異,這種差異可能影響該蛋白的功能。結(jié)論該感染者為長期無進(jìn)展者,Vpu蛋白功能的部分喪失可能與疾病的長期無進(jìn)展有關(guān)。
[Abstract]:Objective to investigate the virological factors influencing the progression of HIV / AIDS by analyzing the sequence characteristics of the virus in those who have no progress after HIV / AIDS (HIV 1) infection for a long time. Methods the viral load of HIV-1 was detected on AmplicorCobas with HIV-1 Monitor Version 1.5 reagents. The near-full-length sequence of HIV-1 was counted on FASCalibur using multitest four-color reagent. The sequence was sequenced by three pairs of primers. ContigExpress software was used for sequence editing and assembly of HIV-1 subtypes. The traceability of HIV-1 was analyzed by on-line REGA HIV-1Subtyping Tool-Version 3.0 software package. Bayesian Markov chain-Monte Carlo MCMCalgorithm was used to construct the phylogenetic tree. The initial epidemic time and region of the HIV-1 strain in China were inferred, and the sequence of HIV-1 proteins was submitted to the SWISS-MODEL protein homology modeling database, and the protein structure was constructed by using http: / swissmodel.expasy.org.interactive. Results the virus load in the latent period was 4.32 脳 10 ~ 3 copies / mL ~ (-1). The CD4 T lymphocytes in peripheral blood were still higher than 500 / 渭 L 10 years after infection. The virus infected by this infection was B subtype. It was first popular in Henan Province in 1992. Compared with the Vpu protein encoded by reference strain HBX2, the Vpu encoded by the virus has a significant difference in structure, which may affect the function of the protein. Conclusion the partial loss of Vpu protein function may be related to the long-term no-progression of the disease.
【作者單位】： 江蘇省疾病預(yù)防控制中心;江蘇省血吸蟲病防治研究所;
【基金】：江蘇省衛(wèi)生廳科教興衛(wèi)工程重點人才課題(RC2011083)~~
【分類號】：R512.91

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