本文選題：慢性乙型肝炎病毒 + HLA-DP　； 參考：《新疆醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：通過檢測候選基因HLA-DP rs3077.rs2301220.rs3135021.rs9277341. rs9277378.rs9277542.rs9277535.rs9277471八個位點單核苷酸多態(tài)性,探討新疆維吾爾族、漢族HLA-DP SNPs與慢性乙型肝炎病毒感染的相關(guān)性；探討HLA-DP基因單核苷酸多態(tài)性是否影響維、漢兩民族之間HBV感染的個體差異。為臨床診斷、治療及預(yù)后提供以分子學(xué)水平為指標的遺傳學(xué)參考依據(jù)。方法：收集新疆醫(yī)科大學(xué)中醫(yī)醫(yī)院、庫爾勒維吾爾醫(yī)院的住院和門診患者以及體檢人員作為受試者。所收樣本分為維族、漢族慢性乙肝患者；維族、漢族健康者共四組,每組人數(shù)均等。采用1：1病例-對照研究,病例組為慢性乙肝患者,對照組為體檢健康人群。1、使用DNA提取試劑盒提取標本基因組DNA并進行質(zhì)檢。2、用SNapshot方法對HLA-DP基因rs3077.rs2301220.rs3135021.rs9277341.rs9277378.rs9277542.rs9277535.rs9277471的八個位點進行檢測及分型。3、統(tǒng)計分析采用SAS9.3。研究中所獲得的數(shù)據(jù),年齡采用均數(shù)和標準差表示,基因型頻率和等位基因頻率采用構(gòu)成比或絕對數(shù)描述,先對維吾爾族與漢族之間進行Hardy-Weinberg平衡檢驗,乙肝組和非乙肝組之間基因多態(tài)性的差異采用χ2檢驗,分別以疾病、性別作為分層因素比較漢族和維吾爾族之間、乙肝非乙肝之間基因多態(tài)性的差異采用Cochran-Mantel-Haenselx2檢驗,不同基因型和有無乙肝之間的多因素分析采用Logistic回歸,檢驗水準α=0.05,1-β=0.8。結(jié)果：1、在HBV+組和健康對照組比較中這三個位點的P值均小于0.05,具有統(tǒng)計學(xué)意義,故其位點多態(tài)性與HBV感染相關(guān)。2、在漢族HBV+與對照組比較時,個位點的P值均小于0.05,具有統(tǒng)計學(xué)意義。3、在漢族健康對照組與維吾爾族健康對照組比較,位點的單堿基分型具有統(tǒng)計學(xué)意義。4、漢族HBV+和維吾爾族HHBV+比較,rs3077這六個位點中單核苷酸多態(tài)性分型具有統(tǒng)計學(xué)意義。5、在性別與乙型肝炎病毒感染的相關(guān)性研究中發(fā)現(xiàn)健康對照組四個位點的單堿基分型是具有統(tǒng)計學(xué)意義的。結(jié)論：1、HLA-DP SNP與乙型肝炎病毒感染的關(guān)系, rs3077、rs2301220、rs9277341的三個位點與乙型肝炎病毒感染相關(guān),其中rs3077GG、 rs2301220TT、rs9277341CC基因型可能增加乙型肝炎感染的風(fēng)險。2、漢族健康對照組和維吾爾族健康對照組比較,發(fā)現(xiàn)rs3077、rs2301220、rs9277341、rs9277378、 rs9277542、rs9277471這六個基因位點具有統(tǒng)計學(xué)意義。其中rs3077AA、rs2301220CC、 rs9277341TT、rs9277378AA、rs9277542TT、rs9277471GG基因型有可能降低乙型肝炎病毒感染的可能性。3、男女性別差異與乙肝病毒感染的關(guān)系,rs9277341、rs9277378、 rs9277542、rs9277471四個位點可能與發(fā)病人群中的那女性別差異相關(guān)。rs9277341CC、 rs9277378GA、rs9277378GG、rs9277542CC基因型有可能增加乙型肝炎感染的風(fēng)險。4、HLA-DP基因的基因頻率是存在民族差異的,HLA-DP基因的rs3077、rs2301220、 rs9277341、rs9277378、rs9277542、rs9277535、rs9277471的七個位點中不同基因A、G、C、T的數(shù)目和基因頻率在維吾爾族與漢族中存在顯著差異,而rs3135021的兩種等位基因A、G的數(shù)目和頻率在兩民族之間無統(tǒng)計學(xué)意義。
[Abstract]:Objective: To explore the correlation between the Xinjiang Uygur, the Han HLA-DP SNPs and the chronic hepatitis B virus infection by detecting the single nucleotide polymorphisms at the eight loci of the candidate gene HLA-DP rs3077.rs2301220.rs3135021.rs9277341. rs9277378.rs9277542.rs9277535.rs9277471, and to explore whether the single nucleotide polymorphism of the HLA-DP gene affects the dimension of the virus. The individual difference of HBV infection among the two ethnic groups. It provides a genetic reference basis for clinical diagnosis, treatment and prognosis. Methods: the hospital of traditional Chinese medicine of Xinjiang Medical University, the hospitalized and outpatient patients in Korla Uygur hospital and the medical staff were selected as subjects. The samples collected were divided into the Uygur ethnic group and the Han chronic B. The liver patients, the Uygur and the Han healthy people were four groups, each group was equal. The 1:1 case control study, the case group were chronic hepatitis B patients, the control group was a healthy population.1, the DNA extraction kit was used to extract the genomic DNA and the quality of the.2 was tested, and the SNapshot method was used for the HLA-DP gene rs3077.rs2301220.rs3135021.rs9277341.rs9277. The eight loci of 378.rs9277542.rs9277535.rs9277471 were detected and typed.3. Statistical analysis adopted the data obtained in the SAS9.3. study. The age was expressed by the average number and standard deviation. The genotype frequency and allele frequency were described by the composition ratio or the absolute number. The Hardy-Weinberg balance test was conducted between the Uygur and the Han nationality. The difference of gene polymorphism between the hepatitis B group and the non hepatitis B group was tested by the x 2 test. The difference of gene polymorphism between the Han and Uygur nationalities was compared with the sex as a stratified factor. The difference of gene polymorphism between hepatitis B and hepatitis B was detected by Cochran-Mantel-Haenselx2 test. The multiple factor analysis between the different genotypes and the hepatitis B and the hepatitis B was Logistic back. The results of alpha =0.05,1- beta =0.8. were as follows: 1, the P values of these three loci in the HBV+ group and the healthy control group were less than 0.05, so the polymorphism of the loci was associated with.2 in HBV infection. The P value of the loci was less than 0.05 in the Han HBV+ and the control group, with statistical significance.3, in the Han healthy control group and in the Han healthy control group. Compared with the Uygur healthy control group, the single base classification of the loci was statistically significant.4, the Han HBV+ and Uygur HHBV+ were compared, and the single nucleotide polymorphisms in the six loci of rs3077 had statistical significance.5. The single base fraction of the four sites in the healthy control group was found in the correlation between sex and hepatitis B virus infection. Conclusion: 1, 1, the relationship between HLA-DP SNP and HBV infection, the three loci of rs3077, rs2301220, rs9277341 are associated with hepatitis B virus infection, and rs3077GG, rs2301220TT, rs9277341CC genotype may increase the risk of hepatitis B infection in.2, Han healthy control group and Uygur health The six loci of rs3077, rs2301220, rs9277341, rs9277378, rs9277542, rs9277471 were statistically significant in the control group. Among them, rs3077AA, rs2301220CC, rs9277341TT, rs9277378AA, rs9277542TT, and the possibility of reducing the possibility of hepatitis B virus infection, sex differences between men and women and HBV infection The four loci of rs9277341, rs9277378, rs9277542, and rs9277471 may be related to.Rs9277341CC, rs9277378GA, rs9277378GG, and rs9277542CC genotypes that may increase the risk of hepatitis B infection, and the gene frequency of the HLA-DP gene is in ethnic differences, HLA-DP gene rs3077, and the rs3077 of HLA-DP gene. The number and frequency of A, G, C, T and gene frequency in the seven loci of rs9277341, rs9277378, rs9277542, rs9277535, and rs9277471 were significantly different between the Uygur and the Han people, while the two alleles of rs3135021 were A, and the number and frequency of G were not statistically significant between the two nationalities.

【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R512.62

【參考文獻】

相關(guān)期刊論文 前1條

1 Thomas F Baumert;Robert Thimme;Fritz von Weizs，

本文編號：1812307