發(fā)布時間：2018-04-02 14:00

  本文選題：脊柱結(jié)核　切入點(diǎn)：白介素-6　出處：《南華大學(xué)》2014年碩士論文

【摘要】：結(jié)核病為一慢性可治愈但死亡率最高之傳染病，特別是脊柱結(jié)核的發(fā)病率與日俱增。近年來耐藥及多重耐藥菌株(multiple drugs resistant-bacilustuberculosis,MDR-TB)的出現(xiàn),更是對現(xiàn)有的抗結(jié)核藥物提出了嚴(yán)重挑戰(zhàn)，尋找新的抗結(jié)核藥，尤其是抗結(jié)核中藥已成為研究方向。有研究顯示IL-6、TNF-a和TGF-β在結(jié)核病變轉(zhuǎn)歸過程中起到重要作用，發(fā)現(xiàn)貓爪草與抗結(jié)核藥物結(jié)合可以提高療效，縮短療程，且沒有產(chǎn)生毒副作用，還能提高患者的免疫力，但目前其機(jī)制不明。 目的：觀察小毛莨內(nèi)酯對脊柱結(jié)核兔病灶組織中白細(xì)胞介素-6（IL-6），腫瘤壞死因子（TNF-a），轉(zhuǎn)化生長因子-β（TGF-β）的表達(dá)水平的影響，探討其治療脊柱結(jié)核的作用機(jī)制。 方法：將未成年兔腰4椎體鉆孔植入結(jié)核標(biāo)準(zhǔn)菌株H37Rv，建立兔脊柱結(jié)核模型。將20只新西蘭未成年兔隨機(jī)分成4組，每組5只。A組，脊柱結(jié)核造模組，不予以任何干預(yù)治療；B組，小毛莨內(nèi)酯治療組，即脊柱結(jié)核造模后給予小毛莨內(nèi)酯灌胃治療；C組：常規(guī)抗癆藥治療組，脊柱結(jié)核造模后予以常規(guī)抗癆藥灌胃治療；D組：常規(guī)抗癆藥聯(lián)合小毛莨內(nèi)酯治療組，脊柱結(jié)核造模后分別予以常規(guī)抗癆藥及小毛莨內(nèi)酯灌胃治療；8周后，取病灶組織，進(jìn)行HE染色和肉眼組織學(xué)觀察，實(shí)時熒光定量PCR反應(yīng)法及免疫印跡法分別檢測小毛莨內(nèi)酯及常規(guī)三聯(lián)抗癆藥治療前后脊柱結(jié)核兔病灶組織中IL-6、TNF-α、TGF-β的mRNA和蛋白表達(dá)水平的變化。結(jié)果應(yīng)用SPSS16.0統(tǒng)計(jì)軟件，進(jìn)行方差分析（on wayANOVA），分析各組間差異， P0.05表示差異性顯著。 結(jié)果：新西蘭白兔腰椎進(jìn)行H37Rv結(jié)核桿菌感染后，結(jié)核病灶組織HE染色顯示組織間有膿細(xì)胞、類上皮樣細(xì)胞或壞死灶等形成，經(jīng)小毛莨內(nèi)酯、常規(guī)抗撈藥以及聯(lián)合用藥治療8周后，結(jié)核病灶區(qū)膿細(xì)胞、類上皮樣細(xì)胞數(shù)量顯著減少；實(shí)時熒光定量PCR反應(yīng)監(jiān)測腰椎結(jié)核病灶組織中脊柱結(jié)核造模組的IL-6、TNF-α、TGF-βmRNA相對表達(dá)量與小毛莨內(nèi)酯治療組、常規(guī)抗撈藥治療組及常規(guī)抗撈藥聯(lián)合小毛莨內(nèi)酯治療組相比較有顯著差異（P0.05），脊柱結(jié)核兔模型經(jīng)過治療后病灶組織中IL-6、TNF-α、TNF-αmRNA表達(dá)明顯降低。常規(guī)抗撈藥聯(lián)合小毛莨內(nèi)酯治療組的IL-6、TNF-α、TGF-βmRNA相對表達(dá)量與小毛莨內(nèi)酯治療組及常規(guī)抗撈藥治療組相比較有顯著差異（P0.05）,且常規(guī)抗撈藥聯(lián)合小毛莨內(nèi)酯治療組中IL-6、TNF-α、TGF-βmRNA的相對表達(dá)量最低，提示采用常規(guī)抗撈藥聯(lián)合小毛莨內(nèi)酯治療后，更能降低組織中IL-6、TNF-α、TGF-βmRNA的表達(dá)水平；免疫印跡法檢測各組椎旁軟組織中IL-6、TNF-α、TGF-β的蛋白表達(dá)水平與熒光定量PCR檢測結(jié)果類似。 結(jié)論： 1.小毛莨內(nèi)酯可以有效降低脊柱結(jié)核兔模型病灶組織中炎癥相關(guān)因子IL-6，，TNF-a的表達(dá)水平，減輕炎性反應(yīng)，降低TGF-β的表達(dá)水平，抑制病灶中肉芽組織形成。 2．小毛莨內(nèi)酯對常規(guī)抗結(jié)核藥治療結(jié)核病有協(xié)同作用。
[Abstract]:Tuberculosis is a chronic curable infectious disease with the highest mortality, especially spinal tuberculosis.Some studies have shown that IL-6, TNF-a and TGF- 尾 play an important role in the process of tuberculosis progression.But at present its mechanism is not clear.Twenty New Zealand minor rabbits were randomly divided into 4 groups: group A (n = 5), group B (n = 5) with spinal tuberculosis, group B without any intervention, group B (n = 5) treated with rhinolactone, group A (n = 5), group B (n = 5) with spinal tuberculosis.The spinal tuberculosis model was treated with routine antituberculous drugs and rhinolactone respectively for 8 weeks. The lesion tissues were taken for HE staining and gross histological observation.The changes of mRNA and protein expression of IL-6 TNF- 偽 TGF- 尾 were detected by real-time quantitative PCR reaction and Western blotting before and after treatment with rhinolactone and conventional anti-tuberculosis drugs in rabbits with spinal tuberculosis.Results using SPSS16.0 statistical software, ANOVA was used to analyze the differences among the groups, which showed significant difference (P0.05).Results: after H37Rv infection in the lumbar vertebrae of New Zealand white rabbits, HE staining showed that there were pyogenic cells, epithelioid cells or necrotic foci between the tissues.After 8 weeks of treatment, the number of pyogenic cells and epithelioid cells in tuberculosis foci decreased significantly.Real-time fluorescence quantitative PCR reaction was used to detect the relative expression of IL-6 TNF- 偽 TGF- 尾 mRNA in the spinal tuberculosis model group and the treatment group.The expression of IL-6 TNF- 偽 TNF- 偽 mRNA in spinal tuberculosis rabbit model was significantly lower than that in the conventional anti-salvage therapy group and the conventional anti-salvage drug combined with rhinolactone treatment group (P0.05), and the expression of IL-6 TNF- 偽 TNF- 偽 was significantly decreased in the spinal tuberculosis model after treatment.The relative expression of IL-6 TNF- 偽 TGF- 尾 mRNA in the conventional anti-salvage drugs combined with Ranunculus milunculatus group was significantly different from that in the Ranunculus milunculatus treatment group and the routine anti-salvage drug treatment group, and the relative expression of IL-6TNF- 偽 TGF- 尾 mRNA was the lowest in the conventional anti-salvage drug combined with Ranunculum milunculate treatment group, and the relative expression of IL-6 TNF- 偽 TGF- 尾 mRNA was the lowest in the conventional anti-salvage drug combined with Ranunculum milunculus lactone treatment group.Conclusion:1.Rhinolactone can effectively reduce the expression of IL-6 and TNF-a in the focal tissue of spinal tuberculosis rabbit model, alleviate the inflammatory reaction, reduce the expression of TGF- 尾, and inhibit the granulation tissue formation in the focus.2.Rhinolactone has a synergistic effect on the treatment of tuberculosis with routine antituberculosis drugs.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R529.2

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相關(guān)期刊論文 前2條

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