本文選題：斯氏按蚊　切入點(diǎn)：微衛(wèi)星標(biāo)記　出處：《昆蟲學(xué)報(bào)》2015年01期

【摘要】：【目的】斯氏按蚊Anopheles stephensi是亞洲東南部城市人體瘧疾的主要媒介,印度12%的瘧疾病例由其引起。本實(shí)驗(yàn)研究了印度中部Madhya Pradesh地區(qū)東北部的瘧疾強(qiáng)化控制(EMCP)區(qū)和非強(qiáng)化控制(非EMCP)區(qū)斯氏按蚊的基因流。在EMCP區(qū),由于采用了各種瘧疾防控措施因而瘧疾病例首先降低,但是很快回升,說明總的瘧疾風(fēng)險(xiǎn)維持穩(wěn)定�！痉椒ā繎�(yīng)用7個(gè)微衛(wèi)星位點(diǎn),對(duì)印度中部Madhya Pradesh地區(qū)東北部的4個(gè)EMCP區(qū)和非EMCP區(qū)采集的斯氏按蚊進(jìn)行基因分型,以分析各種群參數(shù)�！窘Y(jié)果】發(fā)現(xiàn)各標(biāo)記在所有種群中表現(xiàn)出高度的多態(tài)性。在兩區(qū)間未發(fā)現(xiàn)很大的遺傳多樣性。觀察到EMCP區(qū)的東部種群(FST=0.0485,RST=0.1112)比非EMCP區(qū)的北部種群(FST=0.020,RST=0.0145)具有較高的遺傳分化,在EMCP區(qū)和非EMCP區(qū)之間觀察到較高的基因流(12.90,6.16,5.06和2.38)。RST的靈敏度高于FST,說明分化可能是由于突變而非遺傳漂變引起的。【結(jié)論】本研究表明,在EMCP區(qū)和非EMCP區(qū)內(nèi)以及EMCP區(qū)和非EMCP區(qū)之間存在很高的基因流。基因流水平高以及抗蟲性的發(fā)展似乎是EMCP區(qū)和非EMCP區(qū)瘧疾病例發(fā)生增加的重要原因。
[Abstract]:[objective] Anopheles stephensi Anopheles stephensi is a major vector of human malaria in cities in southeastern Asia. In this study, we studied the gene flow of Anopheles stephensi in the northeast of Madhya Pradesh region in central India. As a result of various malaria control measures, malaria cases were first reduced, but recovered quickly, indicating that the overall malaria risk remained stable. [methods] Seven microsatellite sites were used. The genotypes of Anopheles stephensi collected from four EMCP and non-#en3# regions in the northeast of Madhya Pradesh region in central India were genotyped. In order to analyze various population parameters, it was found that the markers showed a high degree of polymorphism in all populations. No significant genetic diversity was found in the two regions. It was observed that the eastern population in the EMCP region was 0.0485 RST-0.1112) and the northern population in the non-#en1# region was 0.020 RST-0.0145). With high genetic differentiation, A higher sensitivity was observed between the EMCP region and the non EMCP region, indicating that the differentiation may be caused by mutation rather than genetic drift. [conclusion] this study shows that the sensitivity of FSTs is higher than that of FSTs. [conclusion] the present study suggests that the differentiation may be caused by mutation rather than genetic drift. [conclusion]. There is a high gene flow between the EMCP region and the non-#en1# region, and between the EMCP region and the non-#en3# region. The high level of gene flow and the development of insecticidal resistance seem to be the important reasons for the increase of malaria cases in EMCP and non-#en5# regions.
【作者單位】： Centre
【基金】：gratefully acknowledge the Department of Biotechnology,Govt. of India for providing financial assistance
【分類號(hào)】：R531.3;Q963

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