本文選題：慢性HBV感染　切入點(diǎn)：微小RNA　出處：《世界華人消化雜志》2016年08期 　論文類型：期刊論文

【摘要】：目的:從microRNAs(miRNAs)調(diào)控角度揭示慢性乙型肝炎病毒(hepatitis B virus,HBV)感染的分子機(jī)制.方法:將符合標(biāo)準(zhǔn)的病例分為慢性HBV感染者組(含慢性乙型肝炎及慢性HBV攜帶者)與正常組,借助Agilent Human miRNA 8×60k微陣列芯片檢測(cè)血漿中miRNAs表達(dá)譜,求得兩組間的差異表達(dá)miRNAs譜(P0.05),借助miRNA生物信息學(xué)分析軟件預(yù)測(cè)其靶基因并對(duì)靶基因進(jìn)行GO功能富集分析和Pathway分析.結(jié)果:兩組間的差異表達(dá)miRNAs共69條(P0.05),28條上調(diào),41條下調(diào);GO分析及Pathway分析得到其功能主要涉及生物黏附、轉(zhuǎn)錄正/負(fù)調(diào)控、生物合成過程的正/負(fù)調(diào)控、氮化合物的代謝過程的正/負(fù)調(diào)控、蛋白質(zhì)定位、蛋白氨基酸的磷酸化、Notch信號(hào)傳導(dǎo)途徑、細(xì)胞凋亡、Wnt信號(hào)通路、Hedgehog信號(hào)通路、T細(xì)胞受體信號(hào)通路、MAPK信號(hào)通路、轉(zhuǎn)化生長(zhǎng)因子b信號(hào)通路、B細(xì)胞受體信號(hào)通路、ErbB信號(hào)通路、p53信號(hào)通路等.結(jié)論:慢性HBV感染受特異性mi RNAs調(diào)控,其調(diào)控涉及多個(gè)生命過程和信號(hào)通路.
[Abstract]:Objective: to elucidate the molecular mechanism of chronic hepatitis B virus infection from the perspective of microRN AsmiRNAs.Methods: the patients with chronic HBV infection (including chronic hepatitis B and chronic HBV carriers) and the normal group were divided into two groups. The expression of miRNAs in plasma was detected by Agilent Human miRNA 8 脳 60k microarray chip. The differentially expressed miRNAs profiles were obtained between the two groups. The target genes were predicted by miRNA bioinformatics analysis software, and the target genes were analyzed by go function enrichment analysis and Pathway analysis. Results: there were 69 differentially expressed miRNAs genes in the two groups. The down-regulation of go analysis and Pathway analysis showed that its function was mainly related to biological adhesion. Positive / negative regulation of transcription, positive / negative regulation of biosynthesis, positive / negative regulation of metabolic process of nitrogen compounds, protein localization, phosphorylation of protein amino acids, Notch signaling pathway, Apoptosis / Wnt signaling pathway / Hedgehog signaling pathway / T cell receptor signaling pathway / MAPK signaling pathway. Transforming growth factor b (TGFb) signaling pathway / B cell receptor pathway / p53 signaling pathway etc. Conclusion: chronic HBV infection is regulated by specific mi RNAs, which involves multiple life processes and signaling pathways.
【作者單位】： 成都中醫(yī)藥大學(xué)附屬醫(yī)院感染科;成都中醫(yī)藥大學(xué);四川大學(xué)華西醫(yī)院感染科;廣元市中醫(yī)院消化科;成都中醫(yī)藥大學(xué)附屬醫(yī)院醫(yī)務(wù)部;成都中醫(yī)藥大學(xué)附屬醫(yī)院科研部;成都市傳染病醫(yī)院感染科;簡(jiǎn)陽市中醫(yī)院消化科;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目,Nos.81202624,81102720~~
【分類號(hào)】：R512.62
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本文編號(hào)：1645072