本文選題：乙型肝炎病毒　切入點(diǎn)：計(jì)劃免疫　出處：《中國輸血雜志》2017年10期 　論文類型：期刊論文

【摘要】：目的了解深圳市在計(jì)劃免疫前后出生的無償獻(xiàn)血者HBV感染情況,分析其血清學(xué)和分子生物學(xué)特征。方法將本中心2016年2-6月收集的26 320人(份)無償獻(xiàn)血者標(biāo)本,以1992年為界分為計(jì)劃免疫前出生組組:19 898人(份),年齡18-24歲;計(jì)劃免疫后出生組:6 422人(份),24-55歲。分別將每組再分為HBs Ag(+)/HBV DNA(+)、HBs Ag(+)/HBV DNA(-)、HBs Ag(-)/HBV DNA(+)和HBs Ag(-)/HBV DNA可疑(NAT初篩陽性、鑒定試驗(yàn)陰性)4種類型,做"乙肝兩對半"檢測與HBV DNA小容量和大容量提取,采用巢氏PCR方法擴(kuò)增BCP/PC和S區(qū)基因序列,并對所得序列做基因型分析,同時(shí)采用實(shí)時(shí)熒光定量PCR檢測(q PCR)和分析。結(jié)果本組26 320(人)份獻(xiàn)血者標(biāo)本,通過酶免方法初篩檢出242例HBs Ag不合格標(biāo)本,經(jīng)NAT、巢氏-PCR和q PCR檢測HBV DNA陽性率為0.741%(195/26 320),其中195名HBV陽性者里有164(130+34)人為初次獻(xiàn)血者。計(jì)劃免疫前后出生2組中初次獻(xiàn)血者的HBV DNA陽性率分別為1.309%(130/9 929)vs 0.707%(34/4 810)(P0.05);隱匿性乙肝感染(OBI)陽性率分別為0.256%(51/19 898)vs 0.093%(6/6 422)(P0.05)。在可分型的120例標(biāo)本中,HBV基因型B型95例、C型25例,2種基因型在2組間(74/19 vs 21/6)的分布無明顯差異(P0.05)。結(jié)論乙肝疫苗的接種明顯降低了獻(xiàn)血人群HBV感染的風(fēng)險(xiǎn),有益于輸血安全保障的提高。
[Abstract]:Objective to investigate the HBV infection in unpaid blood donors born before and after immunization in Shenzhen, and to analyze its serological and molecular biological characteristics. In 1992, there were 19 898 children (age 18-24) in the group of birth before the planned immunization, and 6 422 (24 to 55 years old) in the group of births after the planned immunization. Each group was subdivided into two groups: HBs Ag(-)/HBV (/ / HBDNA) and HBs Ag(-)/HBV DNA (HBs Ag(-)/HBV DNA), respectively. Four types of negative DNA were identified. "Hepatitis B two-and-half" detection and HBV DNA small volume and large volume extraction were performed. The gene sequences of BCP/PC and S region were amplified by nested PCR method, and genotypes were analyzed. At the same time, real-time fluorescence quantitative PCR was used to detect qPCRs and analysis. Results A total of 26 320 (human) blood donor samples were detected by enzyme immunoassay (Elisa), and 242 unqualified samples of HBs Ag were detected by enzyme immunoassay. The positive rate of HBV DNA detected by NATT, Nat's -PCR and Q PCR was 0.741- / 26,320g / 195, of which 164 / 13034 were first-time blood donors. The positive rates of HBV DNA were 1.309% / 9 929 vs 0.70734 / 4 810% P0.05 respectively in the two groups born before and after planned immunization. The positive rates of HBV infection OBI were 0.2566 / 19 898 vs 0.0932 / 6 / 6 / 6 422 / P0.05 respectively. There was no significant difference in the distribution of HBV genotype B genotype B genotype B type B genotype and B genotype B genotype in 25 patients with C genotype between the two groups (P 0.05). Conclusion Hepatitis B vaccine vaccination has a significant decrease in the distribution of HBV genotype B and B genotype in the two groups. Conclusion Hepatitis B vaccine vaccination has a significant decrease in the distribution of HBV genotype B genotype B and B genotype B genotype in 25 patients with C genotype. Conclusion the distribution of hepatitis B vaccine is significantly lower than that of the control group (P 0.05). Lower risk of HBV infection in blood donors, It is beneficial to improve the safety and security of blood transfusion.
【作者單位】： 深圳市血液中心;大連醫(yī)科大學(xué)檢驗(yàn)系;
【基金】：深圳市衛(wèi)生計(jì)生系統(tǒng)科研項(xiàng)目(SZGW2017018)
【分類號】：R512.62
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本文編號：1580640