本文選題：甲型HN流感病毒　切入點(diǎn)：血凝素　出處：《醫(yī)學(xué)研究生學(xué)報(bào)》2017年10期 　論文類型：期刊論文

【摘要】：目的甲型H7N9亞型流感病毒對(duì)人具有較高致死率,了解該病毒在我國(guó)的流行態(tài)勢(shì)具有重要意義。文中旨在研究2013-2017年中國(guó)地區(qū)H7N9亞型禽流感病毒血凝素(HA)基因的變異情況和進(jìn)化特征。方法從全球共享禽流感數(shù)據(jù)倡議組織和美國(guó)國(guó)家生物技術(shù)中心流感病毒數(shù)據(jù)庫(kù)下載2013-2017年中國(guó)地區(qū)甲型H7N9流感病毒HA基因序列,利用生物信息軟件進(jìn)行基因同源性分析、HA分子特征分析、系統(tǒng)進(jìn)化分析及選擇性壓力分析。結(jié)果同源性分析結(jié)果顯示,2013-2017年毒株的HA基因與參考毒株的同源性分別為99.0%~99.9%、98.7%~99.5%、98.4%~99.6%、76.8%~99.4%、69.9%~98.2%,同源性逐年降低。HA分子特征分析結(jié)果顯示,與參考毒株相比,抗原位點(diǎn)發(fā)生變異的有21處,其中A區(qū)的148位、C區(qū)的285位氨基酸位點(diǎn)的突變頻率最高;N285D的變異在2015年最高,達(dá)到23%;R148K的突變率逐年升高,在2016年和2017年分別達(dá)到65%和78.5%。系統(tǒng)進(jìn)化分析結(jié)果顯示,2013-2017年同一年份的H7N9亞型流感病毒毒株在系統(tǒng)進(jìn)化樹當(dāng)中基本呈現(xiàn)集中分布狀態(tài);2013年大部分來自廣東的毒株存在T140A的氨基酸替換。2014年毒株分布范圍最廣。選擇性壓力分析結(jié)果顯示,2015年序列通過FEL和IFEL模型均得到正向選擇壓力位點(diǎn)65,2016年和2017年毒株未得到正向選擇壓力位點(diǎn)。結(jié)論甲型H7N9流感病毒逐年發(fā)生變異,且H7N9的毒株變異來源逐漸增加,加大了預(yù)防和控制的難度,應(yīng)進(jìn)一步加強(qiáng)監(jiān)測(cè),以應(yīng)對(duì)病毒對(duì)宿主的不斷適應(yīng)和突變。
[Abstract]:Objective A H7N9 subtype influenza virus has a high mortality in human. It is important to understand the epidemic situation of H7N9 avian influenza virus in China. The aim of this paper is to study the variation and evolutionary characteristics of H7N9 avian influenza virus hemagglutinin (H7N9) gene in China during 2013-2017. The organization and the National Center for Biotechnology of the United States downloaded the HA gene sequence of influenza A H7N9 virus from 2013-2017 in China. Using bioinformatics software to analyze the homology of genes and the molecular characteristics of HA. Phylogenetic analysis and selective pressure analysis. Results the homology of HA gene and reference strain in 2013-2017 were 99.00.99. 9% and 98.775%, respectively. The homology of HA gene decreased year by year. Compared with the reference strain, the homology of HA gene decreased year by year, compared with the reference strain, and the homology of HA gene decreased year by year, compared with the reference strain, the homology analysis showed that the homology of HA gene and the reference strain were 99.0%, 99.7% and 99.7%, respectively, and the results showed that the homology of HA gene was significantly lower than that of the reference strain. The mutation frequency of the 285 amino acid loci in A region 148 and C was the highest in 2015, and the mutation rate of 23g R148K was increased year by year, and the mutation frequency of N285D was the highest in 2015. It reached 65% and 78.5 in 2016 and 2017, respectively. The results of phylogenetic analysis showed that the H7N9 subtype influenza virus strains in the same year 2013-2017 showed a concentrated distribution in the phylogenetic tree, and most of the strains from Guangdong existed in 2013. Amino acid substitution in T140A. In 2014, the virus strain was the most widely distributed. The results of selective pressure analysis showed that the positive selective pressure sites 65 were obtained by FEL and IFEL models in 2015, and no forward selective pressure sites were obtained in 2016 and 2016 strains. Conclusion A H7N9 influenza virus mutates year by year. The source of strain variation of H7N9 increased gradually, which increased the difficulty of prevention and control, so the surveillance should be further strengthened to cope with the constant adaptation and mutation of the virus to the host.
【作者單位】： 南京大學(xué)生命科學(xué)學(xué)院;江蘇省疾病預(yù)防控制中心急性傳染病科;
【基金】：國(guó)家自然科學(xué)基金(31370079)
【分類號(hào)】：R511.7

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