本文關(guān)鍵詞： 免疫性不育 HBV感染 血清HBV DNA 精漿HBV DNA　出處：《中華男科學(xué)雜志》2017年05期 　論文類(lèi)型：期刊論文

【摘要】：目的:探討HBV感染與免疫性男性不育發(fā)生率的相關(guān)性。方法:篩選3 124例男性不育患者,依照血清乙肝表面抗原(Hbs Ag)結(jié)果將其分為HBV感染組和HBV未感染組。再通過(guò)免疫珠試驗(yàn)(IBT)測(cè)定抗精子抗體將免疫性不育患者又分為HBV感染和未感染的免疫性不育組。統(tǒng)計(jì)分析HBV感染和未感染免疫性不育組陽(yáng)性率的差異、HBV感染和未感染免疫性不育組精液常規(guī)參數(shù)的差異、HBV感染不育患者血清和精漿HBV DNA拷貝數(shù)相關(guān)性、血清和精漿HBV DNA拷貝數(shù)與精液常規(guī)參數(shù)的相關(guān)性以及精漿HBV DNA拷貝數(shù)與免疫性不育發(fā)生率的差異。精子濃度、前向運(yùn)動(dòng)精子(PR)百分率用計(jì)算機(jī)輔助精子分析系統(tǒng),精子形態(tài)檢查用Diff-Quik染色法。HBs Ag檢查用ELISA法,血清和精漿HBV DNA拷貝數(shù)用熒光定量PCR法。結(jié)果:HBV感染組和未感染組免疫性不育陽(yáng)性率分別為20.3%和3.3%,差異有統(tǒng)計(jì)學(xué)意義(χ~2=187.5 P0.01)。HBV感染和未感染免疫性不育組精液量、精子濃度、PR之間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),而兩組間正常精子形態(tài)百分率(MNS)分別為(3.9±1.7)%和(6.3±2.2)%、,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。血清和精漿HBV DNA拷貝數(shù)存在正相關(guān)(rs=0.86,P0.01)。血清和精漿HBV DNA拷貝數(shù)與PR、MNS均呈負(fù)相關(guān)[(r=-0.233,P0.01和r=-0.465,P0.01)和(r=-0.250,P0.01和r=-0.508,P0.01)]。精漿HBV DNA不同拷貝數(shù)量級(jí)組免疫性不育陽(yáng)性率差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:HBV感染可增加免疫性不育的發(fā)生率,并與精子質(zhì)量低下有相關(guān)性。
[Abstract]:Objective: to investigate the correlation between HBV infection and the incidence of immune male sterility. According to the results of serum hepatitis B surface antigen (HBs), the patients were divided into HBV infected group and HBV uninfected group, and the immune infertile patients were divided into HBV infected group and uninfected immune infertility group by detecting anti-sperm antibody by immunological bead test. The difference of semen routine parameters between HBV infected and uninfected immune infertility patients was analyzed statistically. The correlation between HBV DNA copy number in serum and seminal plasma of infertile patients with HBV infection was analyzed. The correlation between HBV DNA copy number in serum and seminal plasma and semen routine parameters, and the difference between seminal plasma HBV DNA copy number and the incidence of immune infertility. Sperm morphology was examined by Diff-Quik staining. HBs Ag was detected by ELISA. The copy number of HBV DNA in serum and seminal plasma was determined by fluorescence quantitative PCR. Results the positive rates of immunological infertility in the two groups were 20.3% and 3.3 respectively (蠂 2 187.5 P 0.01). There was no significant difference in sperm concentration and PR between the two groups (P 0.05), but the percentage of normal sperm morphology between the two groups was 3.9 鹵1.7% and 6.3 鹵2.2%, respectively. The difference was statistically significant (P 0.05). There was a positive correlation between serum and seminal plasma HBV DNA copy number. There was a positive correlation between serum and seminal plasma HBV DNA copy number. There was a negative correlation between the number and the number of PRNs. There was no significant difference in the positive rate of immunological sterility in the seminal plasma HBV DNA groups with different copy orders of magnitude. Conclusion the infection of HBV in seminal plasma can increase the incidence of immune infertility, and increase the incidence of immunological sterility in the seminal plasma of different copy orders of magnitude (P 0.01 and P 0.01, P 0.01, P 0.01). Conclusion the incidence of immunological infertility can be increased by infection of HBV in seminal plasma, and there is no significant difference in the positive rate of immunological sterility among the groups with different copies of seminal plasma HBV DNA. It was associated with low sperm quality.
【作者單位】： 寧波市中醫(yī)院男科實(shí)驗(yàn)室;
【分類(lèi)號(hào)】：R512.62;R698.2
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本文編號(hào)：1535677