本文關(guān)鍵詞： PCR-熒光探針法 慶陽 丙肝 基因型　出處：《中國免疫學(xué)雜志》2017年10期 　論文類型：期刊論文

【摘要】：目的:應(yīng)用PCR-熒光探針法分析慶陽地區(qū)丙型肝炎患者基因型,并對PCR-熒光探針法的各種性能進行評價。方法:收集慶陽地區(qū)289例各種丙型肝炎患者的臨床資料和外周靜脈血,采用PCR-熒光探針法檢測其基因型,并和PCR-反向點雜交法、測序法進行比較。結(jié)果:289份HCV RNA陽性血清標(biāo)本中,PCR-熒光探針法基因型及亞型檢出率為99.3%(287/289),其中1b型139例(48.1%),2a型136例(47.1%),3a型7例(2.4%),3b型5例(1.7%),未分出型2例(0.7%)。PCR-熒光探針法的特異度和準(zhǔn)確度為100%,重復(fù)性良好,并與PCR-反向點雜交法、巢式PCR測序法分型結(jié)果均一致,三種方法的一致率為98.2%(56/57),差異無統(tǒng)計學(xué)意義(P0.05)。1b基因型患者ALT、AST、PLT和HCVRNA(lg)水平均高于2a基因型患者,差異具有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:慶陽地區(qū)HCV基因型呈現(xiàn)多基因型分布特點,主要為1b型和2a型,且2a型和1b型的比例相當(dāng),呈現(xiàn)出2a型比例升高,1b型下降的趨勢;PCR-熒光探針法HCV基因分型敏感度和特異度高,方法簡單,適合臨床實驗中應(yīng)用。
[Abstract]:Objective: to analyze the genotypes of hepatitis C patients in Qingyang area by using PCR- fluorescence probe method and to evaluate the performance of PCR- fluorescence probe method. Methods: the clinical data and peripheral venous blood of 289 patients with hepatitis C in Qingyang area were collected. PCR- fluorescence probe method was used to detect its genotypes, and PCR- reverse dot hybridization was used to detect its genotypes. Results the detection rates of genotypes and subtypes of HCV RNA positive serum samples were 99.3% 287 / 289%, including 139 cases with 1b type 48.1% ~ 2a and 136 cases with 47.1% ~ 3a type, 7 cases with 2.43b type, 5 cases with 2.43b type, and 2 cases with 0.7% PCR-fluorescence probe method. Specificity and accuracy are 100, reproducibility is good, The results were consistent with those of PCR- reverse dot hybridization and nested PCR sequencing. The concordance rate of the three methods was 98.2 / 56 / 57. There was no significant difference in the levels of alt ASTN PLT and HCVRNANa-lg in patients with genotype P0.05. 1b, which were higher than those in patients with genotype 2a. Conclusion: the genotypes of HCV in Qingyang area are characterized by multiple genotypes, mainly 1b and 2a, and the proportion of 2a and 1b is the same. The results showed that the ratio of 2a to 1b increased and decreased. PCR- fluorescence probe method had high genotyping sensitivity and specificity, and the method was simple and suitable for clinical trial.
【作者單位】： 慶陽市人民醫(yī)院檢驗科;
【基金】：甘肅省衛(wèi)生行業(yè)科研計劃項目(GSWSKY-2014-49)
【分類號】：R440;R512.63
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本文編號：1516193