本文關(guān)鍵詞： 腦泡狀棘球蚴病 綿羊 模型　出處：《新疆醫(yī)科大學(xué)》2013年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的：通過(guò)接種鼠源性泡狀棘球蚴感染羊腦，探討建立羊腦泡狀棘蚴（Cerebralalveolar echinococcosis，CAE）病實(shí)驗(yàn)?zāi)Ｐ偷姆椒�，為觀察腦泡狀棘球蚴病形態(tài)學(xué)變化、MRI表現(xiàn)及免疫學(xué)分析提供良好載體。方法：實(shí)驗(yàn)用健康新疆大尾綿羊10只，隨機(jī)分成兩組并編號(hào)，先用DWI序列（b=1000s/mm2）測(cè)量腦內(nèi)各部位的ADC值，再用兩樣本的t檢驗(yàn)分析確保實(shí)驗(yàn)動(dòng)物腦組織結(jié)構(gòu)的一致性。所有實(shí)驗(yàn)動(dòng)物均在直視下顱骨定位穿刺，腦內(nèi)接種泡狀棘球蚴源頭蚴混懸液約0.2ml，分別在第6、12、18個(gè)月后用MRI觀察羊腦泡狀棘球蚴生長(zhǎng)狀況，觀察DWI及MRS表現(xiàn)，剖檢后分析其形態(tài)學(xué)及病理學(xué)特點(diǎn)。結(jié)果：所有實(shí)驗(yàn)動(dòng)物腦組織各部位的ADC值經(jīng)統(tǒng)計(jì)軟件分析后，P0.05，沒(méi)有統(tǒng)計(jì)學(xué)意義，，腦部各測(cè)量部位之間沒(méi)有明顯的組織差異。本實(shí)驗(yàn)經(jīng)MRI及病理證實(shí)，成功建模3只，成蟲(chóng)率30.00%（3/10），MRI檢測(cè)3只均為單發(fā)病灶，T2WI表現(xiàn)為結(jié)節(jié)狀不規(guī)則低信號(hào)，其中2例具有典型“煤炭樣”改變，內(nèi)間雜多發(fā)小囊泡樣稍高信號(hào)；光鏡下觀察病灶區(qū)有大量淋巴細(xì)胞、嗜酸性粒細(xì)胞及漿細(xì)胞浸潤(rùn)，周?chē)⊙荛]塞并伴有炎性反應(yīng)。2例MRI檢查見(jiàn)線條樣稍短T2信號(hào)，解剖后未見(jiàn)明顯病灶，5例MRI未見(jiàn)異常表現(xiàn)。結(jié)論：人工接種鼠源性泡狀棘球蚴原頭蚴懸液建立綿羊腦泡狀棘球蚴病模型具有可行性，方法簡(jiǎn)單，具有可重復(fù)性。
[Abstract]:Objective: to establish an experimental model of Cerebralalveolar echinococcosis (CAEE) disease in sheep by inoculation of rat-derived hydatid alveolar hydatid infection in sheep brain. Methods: ten healthy Xinjiang large tail sheep were randomly divided into two groups and numbered. The ADC values of various parts of the brain were measured with DWI sequence of 1000smm2. Then the t-test analysis of two samples was used to ensure the consistency of the brain structure of the experimental animals. About 0.2 ml of hydatid suspension was inoculated into the brain of Echinococcus alveolaris. MRI was used to observe the growth, DWI and MRS of Echinococcus alveolaris in sheep brain after 6 ~ 12 months and 18 months later, respectively. Results: the ADC values of brain tissues of all experimental animals were analyzed by statistical software (P0.05), and there was no statistical significance. There was no significant tissue difference between the measured sites of the brain. MRI and pathology confirmed that 3 rats were successfully modeled. The adult rate was 30.000 / 10 / 10. All of them were single lesions with irregular low signal intensity on T2WI. The typical "coal like" changes were found in 2 cases, with a little hyperintense signal of multiple vesicles in the interiors, and a large number of lymphocytes, eosinophils and plasma cells infiltrated in the lesion area under light microscope. Peripheral small vessel occlusion with inflammatory response was found in 2. 2 cases of MRI with line like short T 2 signal. Conclusion: it is feasible to establish the animal model of cerebral alveolar echinococcosis in sheep by artificial inoculation of mouse alveolar hydatid suspension, the method is simple and reproducible.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R445.2;R532.32

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