發(fā)布時(shí)間：2018-07-04 17:31

  本文選題：間質(zhì)干細(xì)胞 + 細(xì)胞分化　； 參考：《解放軍醫(yī)學(xué)雜志》2016年08期

【摘要】：目的探討富勒醇對(duì)基于懸滴培養(yǎng)的大鼠脂肪間充質(zhì)干細(xì)胞(r ADSCs)向成骨細(xì)胞分化的影響。方法將rADSCs通過懸滴培養(yǎng)3d形成大鼠脂肪間充質(zhì)干細(xì)胞球,將細(xì)胞球用胰酶消化分散成單細(xì)胞,對(duì)獲得的單細(xì)胞進(jìn)行二維貼壁培養(yǎng)24h,然后更換為成骨誘導(dǎo)培養(yǎng)基培養(yǎng),其中未添加富勒醇組為對(duì)照組,添加1.0μmol/L富勒醇組為實(shí)驗(yàn)組,誘導(dǎo)分化14d及21d后利用茜素紅染色和實(shí)時(shí)定量PCR檢測(cè)脂肪干細(xì)胞球來源的單細(xì)胞向成骨細(xì)胞分化的能力。結(jié)果懸滴培養(yǎng)3d的rADSCs可形成大小均一的微球結(jié)構(gòu),經(jīng)胰酶消化分散可獲得細(xì)胞球來源的單細(xì)胞。與對(duì)照組相比,在成骨誘導(dǎo)培養(yǎng)基中添加1.0μmol/L富勒醇可使所獲單細(xì)胞形成更多的礦化的鈣結(jié)節(jié),且相關(guān)成骨基因Runx2、OCN、ColⅠ的表達(dá)增強(qiáng)。結(jié)論富勒醇可以顯著增強(qiáng)基于懸滴培養(yǎng)獲得的rADSCs的成骨分化,有助于提高其成骨誘導(dǎo)的效率。
[Abstract]:Objective to investigate the effect of fullethanol on differentiation of rat adipose mesenchymal stem cells (r ADSCs) into osteoblasts. Methods Rat adipose mesenchymal stem cell spheres were formed by suspension culture of rADSCs for 3 days. The cells were digested and dispersed into single cells by trypsin. The obtained cells were cultured for 24 hours with two-dimensional adherent culture and then replaced with osteoblast induction medium. The control group was treated with no fullethanol and the experimental group with 1.0 渭 mol / L fulleol. After 14 and 21 days of differentiation induction, alizarin red staining and real-time quantitative PCR were used to detect the ability of single cells derived from adipose stem cells to differentiate into osteoblasts. Results the rADSCs cultured for 3 days could form a uniform structure of microspheres, and a single cell derived from the cells could be obtained by trypsin digestion and dispersing. Compared with the control group, adding 1.0 渭 mol / L fulleol to the osteoblast induction medium resulted in the formation of more mineralized calcium nodules and increased expression of the osteogenic gene Runx2OCNCol 鈪，

本文編號(hào)：2096828