本文選題：NALP3 + MAPK　； 參考：《山西醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的通過(guò)大鼠腎臟組織局部轉(zhuǎn)染NALP3特異性小干擾質(zhì)粒（siRNA）后制作腎臟缺血再灌注損傷（IRI）模型，探討病原體相關(guān)分子構(gòu)象識(shí)別受體NALP3對(duì)大鼠腎臟IRI的影響及其作用機(jī)制。 方法(1)將健康SD大鼠隨機(jī)分為假手術(shù)（sham）組、缺血再灌注（I/R）不同時(shí)間1h、2h、4h、8h、16h、24h組、空質(zhì)粒+sham組、空質(zhì)粒+I/R組和siRNA質(zhì)粒+I/R組，摘除右腎一周后夾閉左腎動(dòng)脈制作腎臟IRI模型，轉(zhuǎn)染組摘除右腎后超聲微泡造影技術(shù)完成轉(zhuǎn)染一周后同樣方法制作IRI模型，各組10大鼠，留取血清及腎組織標(biāo)本。(2)對(duì)腎臟組織進(jìn)行病理?yè)p傷半定量分析；檢測(cè)血清Scr、BUN含量的變化。(3) western印跡法半定量分析腎組織NALP3的表達(dá)變化、ERK、JNK、P38磷酸化程度變化；RT PCR檢測(cè)NALP3mRNA表達(dá)變化。 結(jié)果(1)大鼠缺血再灌注不同時(shí)間均發(fā)生不同程度急性腎小管壞死，各組病理評(píng)分中以缺血再灌注4h組病理?yè)p傷最重（P＜0.01）。(2)大鼠缺血再灌注不同時(shí)間血清Scr、BUN水平均有不同程度升高，以4h組最高。(3)與假手術(shù)組比較，NALP3在缺血再灌注1h、2h、4h、8h、16h、24h表達(dá)顯著增高（P＜0.05），4h達(dá)到最高，之后趨于穩(wěn)定。(4)與單純?nèi)毖俟嘧?h組比較，，siRNA質(zhì)粒+I/R組腎組織病理?yè)p傷明顯減輕，JNK、P38磷酸化程度明顯下降(P＜0.01)，ERK磷酸化程度仍然較高。 結(jié)論1)NALP3在大鼠腎臟缺血再灌注損傷中表達(dá)增加，下調(diào)其表達(dá)后腎臟缺血再灌注損傷明顯改善。2) MAPK通路在大鼠腎臟缺血再灌注損傷中磷酸化程度明顯增強(qiáng)，下調(diào)NALP3后磷酸化程度明顯減少，提示下調(diào)NALP3對(duì)大鼠腎臟缺血再灌注損傷有明顯保護(hù)作用，并且是通過(guò)抑制JNK、P38的活化實(shí)現(xiàn)的。
[Abstract]:Objective to investigate the effects of NALP3 specific small interference plasmid (siRNA) on renal ischemia reperfusion injury (IRI) and its mechanism. Methods (1) healthy Sprague-Dawley rats were randomly divided into sham-operated (sham) group, ischemia reperfusion (I / R) group, empty plasmid sham group, empty plasmid I / R group and siRNA plasmid I / R group. In the transfection group, the IRI model was made one week after transfection with the right post-renal ultrasound microbubble technique. Serum and renal tissue samples were collected from 10 rats in each group. (2) Semi-quantitative analysis of renal injury was carried out. (3) the changes of NALP3 expression in renal tissue were detected by western blotting. (3) the changes of phosphorylation degree of ERKN JNKP P38 in renal tissue were detected by RT-PCR and the expression of NALP3 mRNA was detected by reverse transcription-polymerase chain reaction (RT PCR). Results (1) Acute tubular necrosis occurred at different time after ischemia reperfusion in rats. In the pathological score of each group, the pathological injury was the most serious in the 4 h ischemia reperfusion group (P < 0. 01). (2). The level of serum Scr-bun was increased in different time after ischemia reperfusion in rats. The highest expression of NALP3 was observed in the 4h group (P < 0.05). (3) the expression of NALP3 in the ischemia reperfusion group was significantly higher than that in the sham operation group (P < 0.05), and the expression of NALP3 was significantly increased at 1h, 2h, 4h, 8h, 16h and 24h (P < 0.05). (4) compared with the ischemia reperfusion group for 4 h, the pathological damage of renal tissue in the siRNA plasmid I / R group was significantly reduced (P < 0.01), and the phosphorylation degree of P38 was still higher in the siRNA plasmid I / R group than in the ischemia reperfusion group (P < 0.01). Conclusion 1) the expression of NALP3 in renal ischemia-reperfusion injury was increased, and the renal ischemia-reperfusion injury was improved significantly after down-regulation of NALP3. 2) the phosphorylation of MAPK pathway in renal ischemia-reperfusion injury was significantly increased in rats. The decrease of phosphorylation after down-regulation of NALP3 suggests that down-regulation of NALP3 has an obvious protective effect on renal ischemia-reperfusion injury in rats, and it is achieved by inhibiting the activation of JNKP38.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 姜勇,龔小衛(wèi);MAPK信號(hào)轉(zhuǎn)導(dǎo)通路對(duì)炎癥反應(yīng)的調(diào)控[J];生理學(xué)報(bào);2000年04期

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