本文關(guān)鍵詞： 腹腔間隔室綜合征(ACS) 腹腔內(nèi)高壓(IAH) 肝腎功能 動物模型　出處：《廣東醫(yī)學(xué)》2017年S1期 　論文類型：期刊論文

【摘要】：目的建立SD大鼠腹腔高壓(IAH)/腹腔間隔室綜合征(ACS)動物模型,研究維持20 mm Hg腹腔內(nèi)壓(IAP)時(shí)間長短與SD大鼠肝腎功能損傷的關(guān)聯(lián)性。方法將96只SD大鼠按隨機(jī)數(shù)字表法,隨機(jī)等量分為實(shí)驗(yàn)組(n=48)和對照組(n=48)。實(shí)驗(yàn)組腹腔內(nèi)注入氮?dú)?維持IAP為20 mm Hg水平,建立IAH/ACS模型,并于1/2、1、2、3、4、5 h測定兩組動脈血中丙氨酸氨基轉(zhuǎn)移酶(ALT)、天冬氨酸氨基轉(zhuǎn)移酶(AST)、肌酐(Cr)和尿素氮(BUN)的含量。結(jié)果 IAP為20 mm Hg時(shí),達(dá)到IAH狀態(tài),維持時(shí)間3 h,SD大鼠肝腎功能未出現(xiàn)顯著異常。當(dāng)維持IAH 3 h、5 h時(shí),實(shí)驗(yàn)組BUN值顯著高于對照組,兩組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05);維持IAH 4 h時(shí),實(shí)驗(yàn)組Cr值顯著高于對照組,兩組差異有統(tǒng)計(jì)學(xué)意義(P0.05);當(dāng)維持IAH 5 h時(shí),實(shí)驗(yàn)組ALT及AST值顯著高于對照組,兩組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論在恒定的IAH條件下,隨著維持時(shí)間延長,對SD大鼠肝腎功能的損害逐漸增大。
[Abstract]:Objective to establish an animal model of IAHV / ACSs in SD rats with intraperitoneal hypertension. To study the relationship between the duration of maintaining 20 mm Hg intraperitoneal pressure (IAPs) and the damage of liver and kidney function in SD rats. Methods 96 SD rats were randomly divided into two groups. The experimental group was randomly divided into two groups: the experimental group (n = 48) and the control group (n = 48). Nitrogen was injected into the abdominal cavity of the experimental group and the IAP was maintained at the level of 20 mm Hg. The IAH/ACS model was established. The alanine aminotransferase (alt) and aspartate aminotransferase (AST) in arterial blood were measured at 1 / 2, 1 / 2, 2, 2, 3, 4 and 5 h. Results when IAP was 20 mm Hg, the content of Cr (Cr) and bun (bun) reached the state of IAH and maintained for 3 h. There was no significant abnormality in liver and kidney function in SD rats. When IAH was maintained for 3 h or 5 h, the BUN value of the experimental group was significantly higher than that of the control group, and the difference between the two groups was statistically significant (P 0.05). When IAH was maintained for 4 h, Cr in the experimental group was significantly higher than that in the control group, and the difference between the two groups was statistically significant (P 0.05). When IAH was maintained for 5 h, the ALT and AST values of the experimental group were significantly higher than those of the control group, and the difference between the two groups was statistically significant (P 0.05). With the prolongation of maintenance time, the damage to liver and kidney function of SD rats increased gradually.
【作者單位】： 昆明醫(yī)科大學(xué)第一附屬醫(yī)院胃腸外科;云南省消化病研究所;
【基金】：云南省科學(xué)技術(shù)廳-云南省科技計(jì)劃項(xiàng)目(編號:2014FB021) 云南省衛(wèi)生計(jì)生委-云南省衛(wèi)生科技計(jì)劃項(xiàng)目(編號:2014NS124,2016NS003)
【分類號】：R-332;R656.1
【正文快照】： 2006年世界腹腔間隔室綜合征協(xié)會(World So-ciety for Abdominal Compartment Syndrome,WSACS)對腹腔高壓(IAH)/腹腔間隔室綜合征(ACS)作出了較為規(guī)范的定義[1]:成年危重病患者腹腔內(nèi)壓(IAP)在4~7 mm Hg范圍內(nèi),IAP持續(xù)≥12 mm Hg或反復(fù)出現(xiàn)病理學(xué)表現(xiàn)則為IAH,IAP持續(xù)≥20mm Hg，

本文編號：1487096