發(fā)布時(shí)間：2018-01-01 02:37

  本文關(guān)鍵詞：遠(yuǎn)東疣柄牛肝菌多糖的提取分離、組成分分析及抗氧化性研究　出處：《沈陽(yáng)農(nóng)業(yè)大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 遠(yuǎn)東疣柄牛肝菌 多糖 分離純化 組成分 抗氧化

【摘要】：遠(yuǎn)東疣柄牛肝菌是一種兼具食藥兩用的大型真菌,其色素提取物、多酚提取物等具有一定的生物活性,但多糖的研究還未見(jiàn)報(bào)道。本文以遠(yuǎn)東疣柄牛肝菌為試驗(yàn)材料,對(duì)該種多糖的提取條件進(jìn)行優(yōu)化、對(duì)單糖的組成分分析及抗氧化活性的進(jìn)行初步探究。結(jié)果如下:1.采用熱水浸提法提取遠(yuǎn)東疣柄牛肝菌的多糖(命名為L(zhǎng)EP,下同),響應(yīng)面分析法(RSM)優(yōu)化多糖的提取條件。通過(guò)優(yōu)化得到多糖的最佳提取條件是:液料比33:1 mL/g,提取時(shí)間3h,提取溫度75℃,在此條件下遠(yuǎn)東疣柄牛肝菌多糖的最佳得率是10.30%。2.LEP經(jīng)DEAE-52纖維素柱層析分離和Sephadex G-100凝膠柱層析純化,共得到三個(gè)單一組分LEP-1、LEP-2和LEP-3。其中,LEP-1是中性糖由蒸餾水洗脫獲得,LEP-2和LEP-3是酸性糖由NaCl洗脫獲得。3.紅外光譜法分析LEP-2的組成分。圖譜結(jié)果顯示LEP-2不僅有β-吡喃糖苷鍵,而且還是一種酸性糖,有糖醛酸存在。紅外光譜有如下主要吸收峰:3309.12cm-1、2926.35 cm1、1601.73 cm-1·1416.48 cm-1·1366.77 cm-1、1247.37 cm-1、1035.02 cm-1、918.89 cm-1、796.78 cm-1。說(shuō)明LEP-2中含有O-H、C-H、羰基C=O對(duì)稱及非對(duì)稱區(qū)域、葡萄糖中特有的-OH變角振動(dòng)區(qū)域、β-吡喃糖苷鍵、甘露糖等區(qū)域。4.高效凝膠過(guò)濾色譜法分析LEP-2的分子量和單糖組成。結(jié)果顯示:分子量等于342375 Da;由巖藻糖:阿拉伯糖:半乳糖:葡萄糖:木糖:甘露糖=1.82:0.61:5.14:26:5.41:61.02。5.通過(guò)測(cè)定自由基(·OH、DPPH·、O2-·)清除能力,來(lái)評(píng)價(jià)LEP及純化組分的抗氧化活性。實(shí)驗(yàn)結(jié)果如下:在樣品是1mg/mL時(shí),·OH的清除能力:LEP-1LEP-2LEP-3LEP;對(duì) O2-·的清除能力:LEPLEP-2LEP-1LEP-3;對(duì) DPPH·的清除能力:LEPLEP-3LEP-1LEP-2。實(shí)驗(yàn)結(jié)果證明該種多糖具有一定的抗氧化能力,且與多糖的質(zhì)量濃度之間具有一定的線性劑量關(guān)系。
[Abstract]:Boletus verrucum is a kind of macrofungi with both food and medicine. Its pigment extract polyphenol extract and so on have certain biological activity. However, the study of polysaccharides has not been reported. In this paper, the extraction conditions of the polysaccharides were optimized by using Boletus verrucosa as the experimental material. The composition analysis and antioxidant activity of monosaccharide were studied. The results are as follows: 1. Extraction of polysaccharides from Boletus verrucosa by hot water extraction (named LEP, the same below). Response surface analysis (RSM) was used to optimize the extraction conditions of polysaccharides. The optimum extraction conditions were as follows: the ratio of liquid to material was 33: 1 mL / g, the extraction time was 3 h, and the extraction temperature was 75 鈩，

本文編號(hào)：1362595