陸地棉在全世界棉花生產(chǎn)中占95%,是一種具有天然可紡織纖維的作物,也是一種油料作物。作為一種相比傳統(tǒng)遺傳圖譜更好的工具,全基因組關(guān)聯(lián)分析（GWAS）被廣泛應(yīng)用于研究復(fù)雜性狀的遺傳變異。本研究中,我們利用80K Illumina Infinium檢測半野生陸地棉自然群體高密度的SNPs,采用GWAS技術(shù)對5個纖維質(zhì)量性狀:纖維伸長（FE）,纖維長度（FL）,纖維馬克隆值（FM）,纖維強度（FS）和纖維整齊度（FU）進行分析。對在兩地三年種植的半野生陸地棉材料的纖維性狀進行評價,發(fā)現(xiàn)半野生陸地棉材料的纖維質(zhì)量表現(xiàn)出較高的多樣性。FE值為2-8%平均為6.06%,CV%值為17.32、FL值為19.61-32.8mm,平均為24.48mm,CV%值為8.63、FM值為2.1-6.5,平均為4.33,CV%值為16.65、FS值為18.23-34.00 cN/tex,平均為24.24 cN/tex,CV%值為8.17、FU值為74.1-87.7,平均為81.35,CV%值為2.63。根據(jù)相關(guān)性分析,FL和FU,FL和FS,FU和FS,FM和FE,FE和FS呈正相關(guān),FL和FM,FL和FE,FU... 

【文章來源】：中國農(nóng)業(yè)科學(xué)院北京市

【文章頁數(shù)】：105 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 INTRODUCTION
    1.1 Uses and production of cotton
    1.2 Geographical distribution and morphological characteristics
    1.3 Cotton fibers development
    1.4 Molecular markers in cotton
        1.4.1 Restriction fragment length polymorphism (RFLP)
        1.4.2 Random amplified polymorphic DNA (RAPD)
        1.4.3 Amplified fragment length polymorphism (AFLP)
        1.4.4 Simple sequence repeats (SSR)
        1.4.5 Single nucleotide polymorphism (SNP)
    1.5 Method of QTL mapping
        1.5.1 Linkage mapping
        1.5.2 Linkage mapping in cotton
        1.5.3 Association mapping
            1.5.3.1 Types of association mapping
                1.5.3.1.1 Candidate gene based association mapping
                1.5.3.1.2 Genome-wide association mapping
    1.6 Association mapping in cotton
        1.6.1 SSR based association studies
            1.6.1.1 Fiber quality
            1.6.1.2 Yield and yield components
            1.6.1.3 Biotic and abiotic stress
        1.6.2 SNP based association studies
            1.6.2.1 Fiber quality
            1.6.2.2 Yield and yield components
            1.6.2.3 Cotton seed nutrition
            1.6.2.4 Salt stress
            1.6.2.5 Early Maturity
    1.7 Population structure
    1.8 Linkage disequilibrium (LD)
    OBJECTIVES
CHAPTER 2 MATERIAL AND METHODS
    2.1 Plant materials
    2.2 Field experiment and phenotyping
    2.3 DNA extraction and SNP genotyping
    2.4 Population structure
    2.5 Linkage disequilibrium
    2.6 Genome-wide association study
    2.7 Candidate genes and gene expression
CHAPTER 3 RESULTS
    3.1 Fiber quality analysis
    3.2 SNPs density
    3.3 Population structure
    3.4 Linkage disequilibrium
    3.5 Marker-trait association
        3.5.1 Fiber elongation
        3.5.2 Fiber length
        3.5.3 Fiber micronaire
        3.5.4 Fiber Strength
        3.5.5 Fiber uniformity
    3.6 Potential candidate genes and genes expressions
CHAPTER 4 DISCUSSION
    4.1 Phenotypic performance
    4.2 Population structure
    4.3 Linkage disequilibrium
    4.4 Marker–trait association
    4.5 Candidate genes and genes expression
CHAPTER 5 CONCLUSION
REFERENCES
APPENDIX
ACKNOWLEDGEMENT
RESUME


【參考文獻】：
期刊論文
[1]中國3大主產(chǎn)棉區(qū)棉花品種遺傳多樣性的RAPD及其與農(nóng)藝性狀關(guān)系的研究[J]. 別墅,孔繁玲,周有耀,張光梅,張群遠(yuǎn),王孝綱.  中國農(nóng)業(yè)科學(xué). 2001(06)



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