近年來,微生物殺蟲劑的應(yīng)用越來越受到人們的重視。在昆蟲的病原微生物中,昆蟲病原真菌（EPF）占據(jù)著非常重要的地位,與其他微生物相比,EPF可以很容易地通過表皮滲透進(jìn)入昆蟲體內(nèi),其他微生物需要通過口器攝入才能引起感染。在真菌穿透昆蟲表皮的過程中,昆蟲和病原真菌之間發(fā)生了一系列復(fù)雜的相互作用,最終,病原真菌導(dǎo)致昆蟲死亡。整個(gè)過程中涉及多種生物化學(xué)和生理機(jī)制,雖然以前的研究揭示了其中一些機(jī)制,但很多機(jī)制仍然未被揭示。本研究利用三個(gè)屬（Metarhizium,Isaria和Beauveria）的病原真菌對(duì)三種昆蟲（米象Sitophilus20oryzae.桃蚜Myzus20persicae,豌豆芽Acyrthosiphon20pisum）的致病效率和病理學(xué)進(jìn)行了研究。對(duì)米象毒力最強(qiáng)的菌株進(jìn)行了篩選。結(jié)果表明,在分生孢子劑量為1×10⁷/ml時(shí),各種病原菌的LT₅₀值不同,ME-33（ILT-01）Isaria20cateniannulatus為3.63±0.11天、Qin-2120Beauveria20bassiana為4.17±0.18天Qin-1... 

【文章來源】：西北農(nóng)林科技大學(xué)陜西省 211工程院校 985工程院校 教育部直屬院校

【文章頁數(shù)】：113 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
ABSTRACT
CHAPTER1 Introduction
    1.1 Literature Review,Potential role of Entomopathogenic Fungi in Biological Control of Insect Pests
    1.2 History of biological control and the EPF background
    1.3 EPF Usage,Benefits and Limitations
        1.3.1 Efficacy over the Chemical Pesticides usage
        1.3.2 EPF infection mode advantages over other Entomopathogens
    1.4 Habitats and Uses of Entomopathogenic fungi
    1.5 Characterization of Entomopathogenic Fungi and Biology
    1.6 An overview on EPF Classification
    1.7 Dispersal and host specificity of EPF
    1.8 Myco-bio control of insect pests,progress and development
    1.9 Indigenous EPF,a well option for biological control
    1.10 Current status and economic importance Entomopathogenic Fungi
        1.10.1 Beauveria Species
        1.10.2 Metarhizium Species
        1.10.3 Isaria Species
    1.11 Tested insect models in study
        1.11.1 Rice Weevil(Sitophilus oryzae.)
        1.11.2 Pest Status
        1.11.3 An overview on the Biology and Ecology of Pest
    1.12 Green Peach Aphid(Myzus persicae)
        1.12.1 Pest Status
        1.12.2 Biology and Ecology of Green Peach Aphid
    1.13 Pea Aphid(Acyrthosiphon pisum)
        1.13.1 Pest Status
        1.13.2 Biology and Ecology of Pea Aphid
CHAPTER2 Pathogenicity of the Indigenous isolates Metarhizium,Isaria and Beauveria against the Sitophilus oryzae(Coleoptera:Curculionidae)Under the Lab conditions
    2.1 Introduction
    2.2 Materials and Methods
        2.2.1 Insect culture
        2.2.2 Source of fungal isolates
        2.2.3 Preparation of fungal conidia
        2.2.4 Initial pathogenicity assay for EPF isolates against S.oryzae
        2.2.5 Virulence assay of EPF against S.oryzae
        2.2.6 Statistical analysis
    2.3 Results
        2.3.1 Fungal Isolates tested by Immersion Method at Single Concentration Bioassay
        2.3.2 Fungal Isolates tested via Food Mix Method at Single Concentration Bioassay
        2.3.3 Comparison of Immersion and Food Mix Fungus Isolates
        2.3.4 Multiple conidial concentration bioassays
        2.3.5 LT50 at various conidial concentrations
    2.4 Discussion
    2.5 Conclusion
CHAPTER3 Potential of Four Entomopathogenic Fungi Isolates as Biological Control Agents against two Aphid Species under Laboratory Conditions
    3.1 Introduction
    3.2 Materials and Methods
        3.2.1 Plants and Insects stock
        3.2.2 Preparation of fungus culture,conidial suspension and Gemination test
        3.2.3 Pathogenicity of EPF Isolates
        3.2.4 Concentration–mortality response
        3.2.5 Efficacy of fungal isolates at different temperatures
        3.2.6 Statistical analysis
    3.3 Results
    3.4 Discussion
    3.5 Conclusions
CHAPTER4 Biochemical Study,Post infection effect of Entomopathogenic fungi on the profile activity of some Anti-Oxidative and Detoxifying Enzymes in Acyrthosiphon pisum
    4.1 Introduction
    4.2 Materials and Methods
        4.2.1 Plants and Insects Rearing
        4.2.2 Fungal culture,conidial suspension and viability test
        4.2.3 Bioassay and sample collection
        4.2.4 Samples for Enzymes preparation
        4.2.5 Protein concentration test
    4.3 Detoxification enzymes activity,post exposure to fungal infection
        4.3.1 GS-Glutathione S-Transferase enzyme activity
        4.3.2 CarE-Carboxylesterase enzyme activity
        4.3.3 AKP-Alkaline phosphate enzyme activity
    4.4 Anti-oxidative enzymes activity assay
        4.4.1 SOD-Superoxide dismutase enzyme activity
        4.4.2 POD-Peroxidase enzyme activity
        4.4.3 CAT-Catalase enzyme activity
        4.4.4 Statistical analysis
    4.5 Results and Discussion
        4.5.1 Effect of I.fumosorosea and M.anisopliae isolate on the actitivity of anti-oxidative enzymes on the post infection
        4.5.2 Effect of I.fumosorosea and M.anisopliae isolate on the Detoxification enzymes Post infection
    4.6 Conclusion
CHAPTER5 Evaluation of transcriptome analysis indicates the differential gene expression for Acyrthosiphon pisum as induced by Isaria fumosorosea
    5.1 Introduction
    5.2 Materials and Methods
        5.2.1 Insect and plant stock
        5.2.2 Fungal culture,conidial suspension and sample collection
        5.2.3 Biological assay of A.pisum adults infected with Isaria fumosorosea SP-535 isolate and sample prepration for RNA-Seq
        5.2.4 RNA Isolation cDNA Library and Illumina sequencing
        5.2.5 Alignment and annotation
        5.2.6 Quality control
        5.2.7 Comparative analysis
        5.2.8 Gene functional annotation
        5.2.9 Differential expression analysis,For the samples with biological replicates
        5.2.10 GO enrichment analysis
        5.2.11 KEGG pathway enrichment analysis
    5.3 Results
        5.3.1 Features and Statistics of sequenced cDNA libraries
        5.3.2 Novel genes detection
        5.3.3 Functional annotation
        5.3.4 Overall distribution of gene expression levels in all samples
        5.3.5 Differential expression analysis
        5.3.6 Correlation assessment of biological replicates
        5.3.7 Identification of differentially expressed genes
        5.3.8 Clustering analysis of differentially expressed genes
        5.3.9 GO classification of differentially expressed genes
        5.3.10 KEGG pathway enrichment analysis of differentially expressed genes
    5.4 Discussion
    5.5 Conclusion
CHAPTER6 Discussion and Conclusion
    6.1 General discussion
    6.2 Conclusion
REFERENCES
Acknowledgements
Author’s Introduction


【參考文獻(xiàn)】：
期刊論文
[1]我國古代農(nóng)業(yè)技術(shù)的優(yōu)良傳統(tǒng)之一——生物防治[J]. 彭世獎(jiǎng).  中國農(nóng)業(yè)科學(xué). 1983(01)



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