黃曲霉是腐生真菌,屬于曲霉屬。這種真菌是一種機(jī)會致病菌,能夠污染大多數(shù)種子作物和其他生物,包括動物和人類。更嚴(yán)重的是,黃曲霉由于其次級代謝產(chǎn)物的高毒性而導(dǎo)致死亡或慢性疾病。因此,影響黃曲霉生長和分布的各種因素、黃曲霉毒素生物合成途徑、基因簇以及調(diào)控機(jī)制已成為過去幾十年研究的主題,其中包括響應(yīng)于滲透壓的（MAPK/HOG）途徑促分裂原活化蛋白激酶信號的研究。盡管該途徑已在一些真菌中廣泛研究,但在黃曲霉中,其機(jī)制尚不清楚。Sak A（hog1/hogA）被認(rèn)為是MAPK/HOG途徑的重要部分,為此本研究重點(diǎn)是確定Sak A在黃曲霉中的生物學(xué)功能。在本研究中,通過同源重組的方法進(jìn)行敲除和互補(bǔ)菌株的構(gòu)建,隨后通過1.2mo L/L的氯化鈉（NaCl）、1.2 mo L/L D-山梨醇、以及0.93和0.99水活度誘導(dǎo)滲透壓。實(shí)驗(yàn)發(fā)現(xiàn),與野生型（WT）和互補(bǔ)菌株（ΔAfsakA::AfsakA）相比,基因敲除株（ΔAfsakA）對分生孢子產(chǎn)生顯著影響。q RT-PCR結(jié)果顯示,aba A和brl A基因的表達(dá)水平在ΔAfsakA中,比WT和ΔAfsakA::AfsakA菌株中發(fā)生下調(diào)。研究還發(fā)現(xiàn)... 

【文章來源】：福建農(nóng)林大學(xué)福建省

【文章頁數(shù)】：123 頁

【學(xué)位級別】：碩士

【部分圖文】：

黃曲霉毒素侵入人體循環(huán)和致病的圖解

碩士學(xué)位論文 Osmotic-adaptation response of sakA/hogA gene to aflatoxin biosynthesis, morphologydevelopment and pathogenicity in Aspergillus flavus

碩士學(xué)位論文 Osmotic-adaptation response of sakA/hogA gene to aflatoxin biosynthesis, morphologydevelopment and pathogenicity in Aspergillus flavusMAPKs in Saccharomyces cerevisiae is conserved with GTP-binding proteins, protein kinases,adaptor, scaffold, activating proteins, cell surface proteins, activators, inhibitors and enzymesconnecting the surface of the cell to intracellular regulatory targets activating differentmorphogenetic changes[109, 123]. Saccharomyces cerevisiae has five MAPKs pathways (Figure 1-4) which consist of protein kinases responsible for fungal regulator namely FUS3, KSS1, HOG1,SLT2, SMK1 and they also identify processes and categorize them in different groups likepheromone response, filamentous growth, osmoadaptation, cell wall integrity, and ascosporeformation respectively[123].


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