肝細胞癌差異基因分析及長鏈非編碼RNA的初步驗證 ——基于轉(zhuǎn)錄組芯片的研究
發(fā)布時間：2020-12-11 11:28
研究目的和意義肝細胞癌（Hepatocellular carcinoma,HCC）是世界上高發(fā)的惡性腫瘤之一,其死亡率居惡性腫瘤的第三位。在中國,肝細胞癌的新發(fā)病例數(shù)和死亡率高居世界前列,其中,乙型肝炎病毒感染占大多數(shù)。在廣西地區(qū),肝細胞癌是死亡率最高的惡性腫瘤。由于肝細胞癌起病隱匿、腫瘤生長迅速、轉(zhuǎn)移性高,多數(shù)患者就診時已經(jīng)屬于中晚期。雖然目前肝臟手術(shù)切除或肝移植等治療手段可緩解病人情況,但是術(shù)后腫瘤復發(fā)和轉(zhuǎn)移嚴重影響患者的生活質(zhì)量。因此,深入研究肝細胞癌的發(fā)生、發(fā)展機制,探索針對肝細胞癌早期、特異性診斷標志物和治療靶點十分必要,這將為肝細胞癌的診斷和治療提供有力依據(jù)。肝細胞癌是一種高度異質(zhì)性的基因源性腫瘤。腫瘤基因本身不僅可導致細胞癌變,而且可通過表觀遺傳學水平調(diào)控引起肝細胞的癌變,主要包括甲基化、乙�；�、基因印記、染色體修飾和非編碼RNA調(diào)控等。其中,最近的研究證實,長鏈非編碼RNA（long noncoding rnas,lnc RNAs）在惡性腫瘤發(fā)生、發(fā)展中起到重要作用,如lnc RNA PCA,HOTAIR,H19等。長鏈非編碼RNA是一類長度大于200nt核苷酸RNA,...
【文章來源】：廣西醫(yī)科大學廣西壯族自治區(qū)
【文章頁數(shù)】：138 頁
【學位級別】：博士
【部分圖文】：
芯片實驗流程圖
圖 1-2 (A)lncRNAs 聚類分析；(B)mRNAs 聚類分析。, P 值<0.05;Tumor1-5 是 5 個病人肝細胞癌組織；Paratumor1-55 個匹配正常肝組織分別代表是相對高表達和低表達的 lncRNAs 或 mRNAs。Fig1-2 (A) Hierarchical clustering of lncRNAs; (B) Hierarchical clustering of mRNA2.0, P<0.05.Tumor1-5 means 5 HCC tissues; Paratumor1-5 means 5 pared normal tissues. Rs relatively high expressed lncRNAs or mRNAs; Green color means relatively low expressNAs or mRNAs.
圖 1-2 (A)lncRNAs 聚類分析；(B)mRNAs 聚類分析。 P 值<0.05;Tumor1-5 是 5 個病人肝細胞癌組織；Paratumor1-55 個匹配正常肝組織。別代表是相對高表達和低表達的 lncRNAs 或 mRNAs。Fig1-2 (A) Hierarchical clustering of lncRNAs; (B) Hierarchical clustering of mRNAs.0, P<0.05.Tumor1-5 means 5 HCC tissues; Paratumor1-5 means 5 pared normal tissues. Red relatively high expressed lncRNAs or mRNAs; Green color means relatively low expressedAs or mRNAs.
【參考文獻】：
期刊論文
[1]Hepatocellular carcinoma and macrophage interaction induced tumor immunosuppressionvia Treg requires TLR4 signaling[J]. Jing Yang, Jin-Xiang Zhang, Hui Wang, Guo-Liang Wang, Qing-Gang Hu, Qi-Chang Zheng, Department of Hepatobiliary Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Emergency Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. World Journal of Gastroenterology. 2012(23)
博士論文
[1]長鏈非編碼RNA在肝癌發(fā)生、發(fā)展過程中的作用及臨床意義[D]. 袁聲賢.第二軍醫(yī)大學 2014
本文編號：2910446
【文章來源】：廣西醫(yī)科大學廣西壯族自治區(qū)
【文章頁數(shù)】：138 頁
【學位級別】：博士
【部分圖文】：
芯片實驗流程圖
圖 1-2 (A)lncRNAs 聚類分析；(B)mRNAs 聚類分析。, P 值<0.05;Tumor1-5 是 5 個病人肝細胞癌組織；Paratumor1-55 個匹配正常肝組織分別代表是相對高表達和低表達的 lncRNAs 或 mRNAs。Fig1-2 (A) Hierarchical clustering of lncRNAs; (B) Hierarchical clustering of mRNA2.0, P<0.05.Tumor1-5 means 5 HCC tissues; Paratumor1-5 means 5 pared normal tissues. Rs relatively high expressed lncRNAs or mRNAs; Green color means relatively low expressNAs or mRNAs.
圖 1-2 (A)lncRNAs 聚類分析；(B)mRNAs 聚類分析。 P 值<0.05;Tumor1-5 是 5 個病人肝細胞癌組織；Paratumor1-55 個匹配正常肝組織。別代表是相對高表達和低表達的 lncRNAs 或 mRNAs。Fig1-2 (A) Hierarchical clustering of lncRNAs; (B) Hierarchical clustering of mRNAs.0, P<0.05.Tumor1-5 means 5 HCC tissues; Paratumor1-5 means 5 pared normal tissues. Red relatively high expressed lncRNAs or mRNAs; Green color means relatively low expressedAs or mRNAs.
【參考文獻】：
期刊論文
[1]Hepatocellular carcinoma and macrophage interaction induced tumor immunosuppressionvia Treg requires TLR4 signaling[J]. Jing Yang, Jin-Xiang Zhang, Hui Wang, Guo-Liang Wang, Qing-Gang Hu, Qi-Chang Zheng, Department of Hepatobiliary Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Emergency Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China , Department of Medical Genetics, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. World Journal of Gastroenterology. 2012(23)
博士論文
[1]長鏈非編碼RNA在肝癌發(fā)生、發(fā)展過程中的作用及臨床意義[D]. 袁聲賢.第二軍醫(yī)大學 2014
本文編號：2910446
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