【摘要】：為了確定中國水仙凝集素基因NTL1的抗蚜功能及培育具有抗蚜功能的大花煙草,利用qPCR方法,研究了中國水仙凝集素基因(NTL1)在轉(zhuǎn)基因大花煙草4個株系中的相對表達(dá)情況;利用自然感蚜、活體接種和離體接種方法研究了不同轉(zhuǎn)基因株系的抗蚜能力。結(jié)果表明,NTL1在不同株系中的相對表達(dá)量差異顯著,從高到低依次為3,4,1,2;抗蚜蟲試驗結(jié)果顯示:轉(zhuǎn)基因煙草具有一定抗蚜性,但不同煙草株系的抗蚜能力有所不同;活體轉(zhuǎn)基因煙草單株與離體煙草葉片的平均蚜蟲密度抑制率分別為8.22%~76.61%,4.62%~65.65%;轉(zhuǎn)基因大花煙草可加速蚜蟲的死亡。對比NTL1在不同轉(zhuǎn)基因大花煙草株系中的相對表達(dá)量及抗蚜效果,發(fā)現(xiàn)NTL1在不同株系中的相對表達(dá)量與植株抗蚜性具有高度一致性。
[Abstract]:In order to determine the aphid resistance function of Chinese narcissus lectin gene NTL1 and to cultivate large flower tobacco with aphid resistance, the relative expression of Chinese narcissus lectin gene (NTL1) in four transgenic tobacco lines was studied by qPCR, and the aphid resistance of different transgenic lines was studied by natural aphids, in vivo and in vitro. The results showed that the relative expression of NTL1 in different lines was significantly different, from high to low was 3, 4, 1, 2. The results of aphid resistance test showed that transgenic tobacco had certain aphid resistance, but the aphid resistance ability of different tobacco lines was different, and the average aphid density inhibition rates of single transgenic tobacco and in vitro tobacco leaves were 8.22% 76.61% and 4.62% 65.65%, respectively. the results showed that transgenic tobacco had certain aphid resistance, but the average aphid density inhibition rate of transgenic tobacco was 8.22% 76.61% and 4.62% 65.65%, respectively. Transgenic tobacco can accelerate the death of aphids. The relative expression and aphid resistance of NTL1 in different transgenic tobacco lines were compared. it was found that the relative expression of NTL1 in different lines was highly consistent with the aphid resistance of plants.
【作者單位】： 河北農(nóng)業(yè)大學(xué)園藝學(xué)院;
【基金】：河北省自然科學(xué)基金項目(C2014204131)
【分類號】：Q943.2
，

本文編號：2525142