【摘要】：纖維膠凝蛋白是非特異性免疫系統(tǒng)中的重要分子。通過(guò)轉(zhuǎn)錄組測(cè)序及cDNA末端快速克隆技術(shù)得到一條仿刺參纖維膠凝蛋白基因的全長(zhǎng)cDNA序列,并將其編碼的蛋白命名為仿刺參纖維膠凝蛋白-1。獲得的基因cDNA全長(zhǎng)為1951bp,其中5′-末端非翻譯區(qū)為397bp,3′-末端非翻譯區(qū)為666bp,開(kāi)放閱讀框?yàn)?88bp,編碼295個(gè)氨基酸,N端16個(gè)氨基酸為信號(hào)肽,信號(hào)肽后面有兩個(gè)G-X-Y重復(fù)序列,C端為纖維蛋白素原結(jié)構(gòu)域。采用實(shí)時(shí)熒光定量PCR方法分析了仿刺參纖維膠凝蛋白-1基因在仿刺參幼參不同組織及細(xì)菌脂多糖刺激后的時(shí)序表達(dá)規(guī)律,結(jié)果顯示仿刺參纖維膠凝蛋白-1基因在仿刺參的腸道、呼吸樹(shù)、體腔細(xì)胞和體壁均有表達(dá),且腸道的表達(dá)量最高;脂多糖刺激后,4種組織的仿刺參纖維膠凝蛋白-1基因表達(dá)量均有變化,且以腸道和體壁表達(dá)量的變化最為顯著;此外,仿刺參纖維膠凝蛋白-1基因在仿刺參4種組織中的表達(dá)量變化具有不同的時(shí)序性,表明仿刺參纖維膠凝蛋白-1可能在仿刺參免疫應(yīng)答中起重要作用。
[Abstract]:Fibrin is an important molecule in the nonspecific immune system. A full-length cDNA sequence of fiber cementitious protein gene of Panax quinquefolium was obtained by sequencing of transcriptional group and rapid cloning of cDNA terminal, and the protein encoded by it was named as fibro-cementitious protein of Panax quinquefolium. The full length of the gene cDNA was 1951bp. the untranslated region was 397bp, the untranslated region was 666bp, the open reading frame was 888bp. the N-terminal 16 amino acids were signal peptides, and there were two G-X-Y repeats behind the signal peptides, and the C-terminal was fibrinogen domain. The untranslated region was 397bp, the open reading frame was 888bp, the N-terminal 16 amino acids were signal peptides, and the C-terminal untranslated region was 397 bp. the C-terminal untranslated region was 666bp. The temporal expression of fibronectin-1 gene in different tissues and bacteria of Panax quinquefolium stimulated by lipopolysaccharide was analyzed by real-time fluorescence quantitative PCR. The results showed that fibronectin-1 gene was expressed in the intestine, respiratory tree, somatic cavity cells and body wall of Panax quinquefolium, and the expression of fibronectin-1 gene in intestinal tract was the highest. After lipopolysaccharide stimulation, the expression of fibronectin-1 gene in the four tissues of Panax quinquefolium changed, especially in intestine and body wall. In addition, the expression of fibronectin-1 gene in four tissues of Panax quinquefolium had different timing, indicating that fibronectin-1 may play an important role in the immune response of Panax quinquefolium.
【作者單位】： 遼寧省海洋水產(chǎn)科學(xué)研究院遼寧省海洋水產(chǎn)分子生物學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：遼寧省科技計(jì)劃項(xiàng)目(2014203006) 遼寧省海洋與漁業(yè)廳項(xiàng)目(201605) 大連市科技計(jì)劃項(xiàng)目(2013J21DW025)
【分類(lèi)號(hào)】：S917.4
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本文編號(hào)：2525073