大腸桿菌TorR蛋白對基因entA、sufA、speA和yhiD的表達(dá)調(diào)控作用
發(fā)布時(shí)間：2019-08-09 11:37
【摘要】：細(xì)菌二組分體能感受外界環(huán)境因子的變化,并調(diào)節(jié)一些基因的表達(dá)來使細(xì)菌適應(yīng)環(huán)境變化。我們之前的研究發(fā)現(xiàn)大腸桿菌二組分體TorR/Tor S中的應(yīng)答蛋白TorR聚集成焦點(diǎn)(TorR focus)定位在細(xì)胞舊極,并且TorR焦點(diǎn)能夠以依賴于細(xì)胞周期的方式與染色體DNA共定位,而且通過這種方式來調(diào)節(jié)一些基因的表達(dá)。這些基因包含F(xiàn)e~(2+)轉(zhuǎn)運(yùn)相關(guān)基因,鐵硫簇組裝相關(guān)基因等。本研究旨在探究TorR蛋白調(diào)控基因表達(dá)的機(jī)制。發(fā)現(xiàn)TorR在細(xì)胞周期特定時(shí)期抑制entA、sufA、speA和yhiD基因的表達(dá),并且這種抑制作用是可能通過結(jié)合到entA、sufA、speA和yhiD的啟動(dòng)子區(qū)域來實(shí)現(xiàn)的。另外,sufA基因的表達(dá)可能同時(shí)受到TorR和Fur蛋白的調(diào)控,但是體內(nèi)試驗(yàn)證明TorR與Fur蛋白之間并不能發(fā)生相互作用。
[Abstract]:The two components of bacteria can feel the changes of external environmental factors and regulate the expression of some genes to make bacteria adapt to environmental changes. Our previous studies have found that the response protein TorR in E. coli TorR/Tor S aggregates into a focal (TorR focus) located in the old pole of the cell, and the TorR focus can co-locate with chromosome DNA in a cell cycle dependent manner, and in this way regulate the expression of some genes. These genes include Fe~ (2) transport related genes, iron sulfur cluster assembly related genes and so on. The purpose of this study was to explore the mechanism of TorR protein regulating gene expression. It was found that TorR inhibited the expression of entA,sufA,speA and yhiD genes during a specific period of cell cycle, and this inhibition may be achieved by binding to the promoter regions of entA,sufA,speA and yhiD. In addition, the expression of sufA gene may be regulated by both TorR and Fur proteins, but in vivo experiments have shown that there is no interaction between TorR and Fur proteins.
【作者單位】： 內(nèi)蒙古自治區(qū)人民醫(yī)院
【基金】：內(nèi)蒙古自治區(qū)自然科學(xué)基金項(xiàng)目(2014MS0803) 內(nèi)蒙古自治區(qū)人民醫(yī)院院內(nèi)基金項(xiàng)目(2016105)共同資助
【分類號】：Q78
本文編號：2524764
[Abstract]:The two components of bacteria can feel the changes of external environmental factors and regulate the expression of some genes to make bacteria adapt to environmental changes. Our previous studies have found that the response protein TorR in E. coli TorR/Tor S aggregates into a focal (TorR focus) located in the old pole of the cell, and the TorR focus can co-locate with chromosome DNA in a cell cycle dependent manner, and in this way regulate the expression of some genes. These genes include Fe~ (2) transport related genes, iron sulfur cluster assembly related genes and so on. The purpose of this study was to explore the mechanism of TorR protein regulating gene expression. It was found that TorR inhibited the expression of entA,sufA,speA and yhiD genes during a specific period of cell cycle, and this inhibition may be achieved by binding to the promoter regions of entA,sufA,speA and yhiD. In addition, the expression of sufA gene may be regulated by both TorR and Fur proteins, but in vivo experiments have shown that there is no interaction between TorR and Fur proteins.
【作者單位】： 內(nèi)蒙古自治區(qū)人民醫(yī)院
【基金】：內(nèi)蒙古自治區(qū)自然科學(xué)基金項(xiàng)目(2014MS0803) 內(nèi)蒙古自治區(qū)人民醫(yī)院院內(nèi)基金項(xiàng)目(2016105)共同資助
【分類號】：Q78
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 姚遠(yuǎn);喬佳鑫;李靜;李慧;莫日根;;大腸桿菌TorS/TorR二組分體應(yīng)答蛋白TorR對DNA復(fù)制起始的影響[J];遺傳;2015年03期
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