【摘要】：為揭示白樺(Betula platyphylla×B.pendula)中Bp AP1轉(zhuǎn)錄因子的調(diào)控機(jī)理,以Bp AP1過(guò)表達(dá)、抑制表達(dá)轉(zhuǎn)基因白樺以及非轉(zhuǎn)基因?qū)φ瞻讟?NT)為試材,對(duì)前期獲得的Bp AP1轉(zhuǎn)基因白樺中的6條開花相關(guān)基因(BpPI、Bp MADS4、Bp MADS5、FT、TFL1和LFY)進(jìn)行了定量PCR分析,探討了白樺Bp AP1轉(zhuǎn)錄因子與這6條開花基因的表達(dá)調(diào)控關(guān)系以及它們對(duì)白樺提早開花的影響。結(jié)果表明:非轉(zhuǎn)基因?qū)φ张cBp AP1抑制表達(dá)轉(zhuǎn)基因白樺葉片中Bp AP1、Bp PI、Bp MADS4、Bp MADS5、FT、TFL1和LFY的相對(duì)表達(dá)量在不同發(fā)育時(shí)期變化均不顯著。Bp AP1過(guò)表達(dá)轉(zhuǎn)基因白樺葉片和雄花序中這7條基因的表達(dá)量在不同發(fā)育時(shí)期均顯著高于NT和Bp AP1抑制表達(dá)轉(zhuǎn)基因白樺。在Bp AP1過(guò)表達(dá)轉(zhuǎn)基因白樺中,除Bp AP1和TFL1外,其他5條基因在各個(gè)時(shí)期的雄花序中表達(dá)量均為最高,多數(shù)基因的表達(dá)高峰出現(xiàn)在6月15日;另外,Bp AP1、Bp MADS4、Bp MADS5和Bp PI在8月15日的雄花序中表達(dá)量也很高;TFL1的相對(duì)表達(dá)量在9月1日的葉片中顯著上調(diào);LFY基因在生長(zhǎng)發(fā)育最旺盛的6、7月份的雄花序中達(dá)到了表達(dá)高峰。Bp AP1與Bp PI、Bp MADS4、Bp MADS5、FT、TFL1、LFY這6條開花相關(guān)基因之間均呈極顯著的正相關(guān)。
[Abstract]:In order to reveal the regulation mechanism of Bp AP1 transcription factor in Betula platyphylla (B. penula), Bp AP1 was overexpressed, the expression of transgenic birch and non-transgenic control birch (NT) was used as a test material, and 6 flowering related genes (BpPI, Bp MADS4, Bp MADS5, The relationship between the transcription factor of Betula platyphylla and the expression of these six flowering genes and their effect on the early flowering of Betula platyphylla were investigated by quantitative PCR. The results showed that the relative expression of Bp AP1, Bp PI, Bp MADS4, Bp MADS5, FT, TFL1 and LFY in the transgenic birch leaves was not significant in different developmental stages. The expression of Bp AP1 overexpressing the 7 genes in the transgenic birch leaves and the male inflorescences was significantly higher than that of NT and Bp AP1 in different developmental stages. In Bp AP1 overexpressing transgenic birch, in addition to Bp AP1 and TFL1, the expression of five genes in the male inflorescence of each period was the highest, and the expression peak of most genes appeared on June 15; in addition, Bp AP1, Bp MADS4, The expression of Bp MADS5 and Bp PI in the male inflorescence on Aug.15 was also high; the relative expression of TFL1 was up-regulated in the leaves of September 1, and the LFY gene reached the peak in the male inflorescence of the most vigorous growth and development of 6 and July. Bp AP1 was positively correlated with Bp PI, Bp MADS4, Bp MADS5, FT, TFL1 and LFY.
【作者單位】： 林木遺傳育種國(guó)家重點(diǎn)實(shí)驗(yàn)室(東北林業(yè)大學(xué));
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31570647)
【分類號(hào)】：S792.153
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本文編號(hào)：2502328