【摘要】：通過(guò)將人肺癌A549細(xì)胞分為正常對(duì)照組和給藥組,采用實(shí)時(shí)定量PCR對(duì)MDR1的表達(dá)水平進(jìn)行測(cè)定。運(yùn)用microRNA芯片對(duì)給藥組和對(duì)照組進(jìn)行microRNA表達(dá)譜分析;采用Targetscan預(yù)測(cè)上調(diào)microRNA中與多藥耐藥基因MDR1相關(guān)的miRNA以及實(shí)時(shí)定量PCR的方法對(duì)篩選出的miRNA進(jìn)行驗(yàn)證,研究丹酚酸A逆轉(zhuǎn)肺癌多藥耐藥基因MDR1相關(guān)microRNA。實(shí)驗(yàn)結(jié)果表明,與對(duì)照組相比,給藥組MDR1的表達(dá)水平明顯下調(diào);microRNA芯片對(duì)人肺癌A549細(xì)胞的給藥組和對(duì)照組進(jìn)行了miRNA表達(dá)譜分析,共篩出426個(gè)差異表達(dá)的miRNA;然后對(duì)差異表達(dá)上調(diào)的miRNA進(jìn)行靶基因預(yù)測(cè),發(fā)現(xiàn)有4個(gè)明顯上調(diào)的miRNA與多藥耐藥基因MDR1相關(guān)。對(duì)4個(gè)miRNA進(jìn)行實(shí)時(shí)定量PCR驗(yàn)證,結(jié)果與芯片一致。因此認(rèn)為丹酚酸A下調(diào)肺癌多藥耐藥基因MDR1可能是通過(guò)影響miRNA表達(dá)進(jìn)而調(diào)控靶基因,對(duì)進(jìn)一步闡明中藥逆轉(zhuǎn)多藥耐藥作用機(jī)制提供了實(shí)驗(yàn)依據(jù)。
[Abstract]:Human lung cancer cell line A549 was divided into normal control group and administration group. The expression of MDR1 was measured by real-time quantitative PCR. MicroRNA chip was used to analyze the expression profile of microRNA in the administration group and the control group. Targetscan was used to predict the up-regulation of miRNA related to multi-drug resistance gene MDR1 in microRNA and real-time quantitative PCR was used to verify the selected miRNA. To study the reversal of lung cancer multi-drug resistance gene MDR1-related microRNA. by Salvianolic acid A. The results showed that compared with the control group, the expression level of MDR1 in the administration group was significantly down-regulated, and the miRNA expression profile of human lung cancer cell line A549 cells was analyzed by microRNA microarray, and a total of 426differentially expressed miRNA; were screened out. Then the target gene of differentially expressed miRNA was predicted, and it was found that four up-regulated miRNA were associated with multi-drug resistance gene MDR1. Four miRNA were verified by real-time quantitative PCR, and the results were consistent with those of the chip. Therefore, it is suggested that Salvianolic acid A down-regulate the expression of multi-drug resistance gene MDR1 in lung cancer by affecting the expression of miRNA and then regulating the target gene, which provides an experimental basis for further elucidating the mechanism of reversal of multi-drug resistance by traditional Chinese medicine.
【作者單位】： 南京中醫(yī)藥大學(xué)基礎(chǔ)醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81503486,81273638) 江蘇省中醫(yī)藥局科技重點(diǎn)項(xiàng)目(ZD201502) 江蘇省高校自然科學(xué)研究項(xiàng)目(14KJD360004)
【分類(lèi)號(hào)】：R285

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