【摘要】：果糖-1,6-二磷酸醛縮酶FBA家族(fructose-l,6-bisphosphate aldolase)在植物響應(yīng)逆境調(diào)控中具有重要的意義。該研究基于天山雪蓮低溫轉(zhuǎn)錄組研究,采用RT-PCR方法克隆了1個(gè)sikFBA1基因,ORF長度為1 077bp,共編碼358個(gè)氨基酸,含有1個(gè)保守的Glycolytic結(jié)構(gòu)域,具有典型的FBA家族特征。系統(tǒng)進(jìn)化分析發(fā)現(xiàn),sikFBA1蛋白分類上屬于Ⅰ型FBA,與來自四葉參(Codonopsis lanceolata)的同源蛋白親緣關(guān)系最近。亞細(xì)胞定位結(jié)果表明,sikFBA1蛋白定位于細(xì)胞質(zhì),屬于胞質(zhì)型同工酶,與預(yù)測一致。實(shí)時(shí)定量PCR結(jié)果顯示,天山雪蓮經(jīng)過不同低溫(4℃/-2℃)處理,sikFBA1基因的表達(dá)水平整體下調(diào),但該基因在冷脅迫與冰凍脅迫、冷馴化與非冷馴化下呈現(xiàn)出不同的表達(dá)模式。研究表明,sikFBA1基因參與了天山雪蓮低溫脅迫的響應(yīng)。
[Abstract]:Fructose-1, 6-diphosphate aldolase FBA family (fructose-l,6-bisphosphate aldolase) plays an important role in plant response to stress. In this study, a sikFBA1 gene was cloned by RT-PCR, which encodes 358 amino acids and contains a conserved Glycolytic domain, which has typical FBA family characteristics, based on the study of low temperature transcriptional group of Xuelian in Tianshan. The ORF gene is 1077bp in length and encodes a total of 358amino acids. Phylogenetic analysis showed that sikFBA1 proteins belonged to type I FBA, and were closely related to homologous proteins from (Codonopsis lanceolata) of Panax quinquefolium. The results of subcellular localization showed that sikFBA1 protein was located in cytoplasm and belonged to cytoplasmic isoenzyme, which was consistent with the prediction. The results of real-time quantitative PCR showed that the expression level of sikFBA1 gene was down-regulated after different low temperature treatment (4 鈩，

本文編號：2493444