【摘要】：高粱是重要五大糧食作物之一,因其對很多化學除草劑相當敏感,噴施時需掌握一定的技巧,一般農戶不易把握,而培育抗除草劑高粱是重要的途徑之一。本研究克隆了玉米品種B73的2個乙酰乳酸合成酶(ALS)基因,進行生物信息學分析高粱、玉米、水稻和擬南芥的ALS基因進化關系、保守結構域以及保守序列,然后進行單、雙人工點突變處理ALS基因,最后構建單雙點突變超表達載體轉化高粱。盡管轉化高粱失敗,本研究也為進一步培育抗除草劑高粱育種新材料奠定了良好的基礎。
[Abstract]:Sorghum is one of the five important food crops, because it is very sensitive to many chemical herbicides, it is necessary to master certain skills when spraying, and it is difficult for farmers to grasp, and cultivating herbicide resistant sorghum is one of the important ways. In this study, two acetyllactic acid synthase (ALS) genes of corn variety B73 were cloned, and the evolutionary relationship, conserved domains and conserved sequences of ALS gene in sorghum, corn, rice and Arabidopsis thaliana were analyzed by bioinformatics. ALS gene was treated with double point mutation, and finally a single and double point mutation overexpression vector was constructed to transform sorghum. Although the transformation of sorghum failed, this study also laid a good foundation for the further cultivation of new herbicide resistant sorghum breeding materials.
【作者單位】： 遼寧省農業(yè)科學院作物分子改良實驗室;
【基金】：遼寧省青年科技創(chuàng)新人才項目(2014030) 高粱農業(yè)產業(yè)技術體系項目(CARS-06-01-06)共同資助
【分類號】：S514

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