【摘要】：【目的】以鹽生植物鹽穗木為材料,研究不同引物對(duì)和不同退火溫度對(duì)鹽穗木內(nèi)參基因β-actin擴(kuò)增效率的影響�！痉椒ā吭O(shè)計(jì)擴(kuò)增β-actin基因的兩對(duì)引物,標(biāo)記為β-actin1和β-actin2,分別建立這兩對(duì)引物擴(kuò)增鹽穗木β-actin基因和特異性引物擴(kuò)增該物種的過(guò)氧化物酶基因POD(鹽響應(yīng)的代表性基因),在不同退火溫度下的標(biāo)準(zhǔn)曲線和擴(kuò)增曲線。【結(jié)果】不論55還是58℃退火溫度,引物對(duì)β-actin1比引物對(duì)β-actin2有更好的擴(kuò)增效率;在55℃退火溫度下,引物對(duì)β-actin1有更高的擴(kuò)增效率。在這兩個(gè)退火溫度下,分別以β-actin1和β-actin2引物對(duì)擴(kuò)增β-actin基因作為內(nèi)參,鹽穗木POD基因的相對(duì)表達(dá)水平具有一定的差異性。【結(jié)論】引物和退火溫度會(huì)影響熒光定量PCR的擴(kuò)增效率,進(jìn)而會(huì)影響靶基因的相對(duì)表達(dá)水平。
[Abstract]:[objective] to study the effects of different primer pairs and annealing temperature on the amplification efficiency of 尾-actin gene in halophyte salted wood. [methods] two pairs of primers for amplification of 尾-actin gene were designed. 尾-actin1 and 尾-actin2, were used to amplify 尾-actin gene and specific primer POD (representative gene of salt response), respectively. The standard curve and amplification curve at different annealing temperatures. [results] the amplification efficiency of primer pair 尾-actin1 was better than that of primer pair 尾-actin2 regardless of 55 鈩，

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