【摘要】：結(jié)合分子標(biāo)記輔助輪回選擇和田間鑒定的方法,將三黃占2號(hào)的抗稻瘟病基因Pi-GD-1(t)和Pi-GD-2(t)(分別簡(jiǎn)稱G1和G2)、CBB23中的抗白葉枯病基因Xa23(簡(jiǎn)稱X)和IR65482-7-216-1-2-B(簡(jiǎn)稱IR65482)的抗褐飛虱基因Bph18(t)(簡(jiǎn)稱B)導(dǎo)入溫恢845、溫恢117和溫恢143等3個(gè)中秈恢復(fù)系,獲得了8個(gè)兼抗稻瘟病和褐飛虱聚合系,溫恢845-G1-G2-B-4、溫恢845-G1-G2-B-5、溫恢117-G1-G2-X-B-3、溫恢143-G1-G2-B-3、溫恢143-G2-X-B-9、溫恢143-G2-X-B-10、溫恢143-G1-G2-B-11和溫恢143-G1-G2-B-37。這些聚合系及其與不育系五豐A的測(cè)交種,對(duì)稻瘟病和褐飛虱的抗性水平接近或略低于稻瘟病抗性親本三黃占2號(hào)和稻飛虱抗性親本IR65482。部分改良恢復(fù)系如溫恢117-G1-G2-X-B-3、溫恢143-G2-X-B-9和溫恢143-G2-X-B-10及其測(cè)交種對(duì)白葉枯病表現(xiàn)為抗病或中抗。改良恢復(fù)系及其測(cè)交種在正常條件下的農(nóng)藝性狀與原始恢復(fù)系及其測(cè)交種相仿或更優(yōu),具有生產(chǎn)應(yīng)用價(jià)值。研究結(jié)果表明,Xa23在不同恢復(fù)系背景下抗性表達(dá)完全,而Pi-GD-1(t)、Pi-GD-2(t)和Bph18(t)對(duì)稻瘟病和褐飛虱抗性的改良效果與恢復(fù)系的遺傳背景有關(guān)。
[Abstract]:Combined with molecular marker assisted reincarnation selection and field identification, the rice blast resistance genes Pi-GD-1 (t) and Pi-GD-2 (t) (of Sanhuangzhan 2 were abbreviated as G 1 and G 2, respectively. The brown planthopper resistance genes Xa23 (X) and IR65482-7-216-1-2-B (IR65482) in CBB23 were introduced into three medium indica restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143.The resistant genes Bph18 (t) (B), which are resistant to bacterial blight, were introduced into three medium indica restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143, respectively. Eight fusion lines resistant to rice blast and brown planthopper were obtained, Wenhui 845-G1 鈮，

本文編號(hào)：2480428