近交對馬氏珠母貝生長性狀、遺傳多樣性及礦化基因表達的影響
發(fā)布時間:2019-05-14 23:17
【摘要】:【目的】探討近交對馬氏珠母貝生長性狀、遺傳結(jié)構(gòu)及礦化基因表達的影響,闡明近交引起子一代性狀退化的遺傳原理,為貝類品種培育提供參考依據(jù)!痉椒ā恳詢蓚黃殼色全同胞家系(M和N)為親本,按照因子設(shè)計構(gòu)建4個家系組合(F1:M1♀×M1♂;F2:M1♀×N1♂;F3:N1♀×M1♂;F4:N1♀×N1♂),比較4個家系成貝生長性狀差異,利用9對SSR引物分析其遺傳多樣性,并以實時熒光定量PCR檢測家系間礦化基因nacrein、pearlin和pif177的表達差異。【結(jié)果】4個家系間的平均殼長、平均殼高、平均殼寬、平均體重和平均殼重存在明顯差異,且近交家系(F1和F4)的平均殼長、平均殼高、平均殼寬、平均體重和平均殼重均小于雜交家系(F2和F3),其生長性狀退化率(ID)為11.7%~29.4%。家系F1、F2、F3和F4的平均觀測雜合度分別為0.2525、0.3116、0.3178和0.2752,平均期望雜合度分別為0.3451、0.3822、0.4005和0.3549,平均多態(tài)信息含量(PIC)分別為0.4591、0.5389、0.4878和0.4810。雜交家系(F2和F3)礦化基因nacrein、pearlin和pif177的相對表達量均高于近交家系(F1和F4),且4個家系的生長性狀平均值與3個礦化基因的相對表達量存在明顯正相關(guān)!窘Y(jié)論】近交能導(dǎo)致馬氏珠母貝生長性狀、遺傳多樣性及礦化基因表達量明顯降低,因此制定馬氏珠母貝育種方案時應(yīng)構(gòu)建合理的交配組合系統(tǒng),防止因近交導(dǎo)致性狀退化與遺傳多樣性指標降低而影響育種進程。
[Abstract]:[objective] to investigate the effects of inbreeding on the growth traits, genetic structure and mineralization gene expression of Pinctada marinifera, and to elucidate the genetic principle of the degradation of traits in the first generation caused by inbreeding. [methods] two yellow shell full sibling families (M and N) were used as parents to construct four family combinations (F1:M1 鈮,
本文編號:2477125
[Abstract]:[objective] to investigate the effects of inbreeding on the growth traits, genetic structure and mineralization gene expression of Pinctada marinifera, and to elucidate the genetic principle of the degradation of traits in the first generation caused by inbreeding. [methods] two yellow shell full sibling families (M and N) were used as parents to construct four family combinations (F1:M1 鈮,
本文編號:2477125
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