一株牛源污蠅桿菌的分離鑒定、耐藥性及耐藥基因檢測(cè)
[Abstract]:(Wohlfahrtiimonas chitiniclastica) is a kind of rare zoonotic pathogen. In October 2016, pus occurred in the eyelids of a fattening cow in a beef cattle farm in Jilin Province, and the effect of using a variety of antibiotics was not good. The eyelid secretions of diseased cattle were collected aseptically, and the bacteria were isolated and cultured. After culture characteristics, staining microscopic examination, biochemical test and 16SrRNA sequence analysis, the isolated strain was identified as Drosophila melanogaster, which was pathogenic to mice. The drug resistance of 20 kinds of antibiotics to the isolated strains was determined by K / B disk diffusion method. The results showed that they were resistant to penicillin, cefradine and erythromycin, and sensitive to the other 17 kinds of drugs. 尾-lactam resistance gene (TEM,ADC,OXA-23,OXA-51), macrolide resistance gene (ermA,ermX) and tetracycline resistance gene (tetK,tetW) were detected respectively.
【作者單位】: 吉林農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院;
【基金】:吉林省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)(2015-24) 吉林省動(dòng)物疾病防治研究創(chuàng)新團(tuán)隊(duì)(20111820) 國(guó)家科技支撐計(jì)劃項(xiàng)目(2014BAK19B005) 吉林省科技成果轉(zhuǎn)化項(xiàng)目(20140309012NY)
【分類號(hào)】:S852.61
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