【摘要】：V-ATPase在植物適應(yīng)逆境中起著非常重要的作用,本研究以重要模式植物擬南芥V-ATPase相關(guān)基因為目標(biāo)序列,通過在6個禾本科植物(水稻,大麥,玉米,高粱,谷子和二穗短柄草)基因組中進(jìn)行比較分析,揭示該基因家族擴(kuò)增機(jī)制和適應(yīng)性進(jìn)化進(jìn)行研究。分析發(fā)現(xiàn),七個不同的植物基因組雖然經(jīng)歷了不同全基因組加倍,其中谷子與玉米加倍關(guān)聯(lián)的基因數(shù)量最多,但V-ATPase基因數(shù)量在每個基因組非常相近,尤其是禾本科中玉米相對其它物種多一次單獨的特異性加倍,然而這一基因家族數(shù)量并未增加,這表明全基因組加倍雖然在一定程度上使得家族基因擴(kuò)增,但是基因丟失可能也維持了該基因組的劑量;對七個基因組中的161個V-ATPase基因進(jìn)行motif分類確定,不同物種基因組中的motif結(jié)構(gòu)不同,擬南芥和水稻中c-亞基相關(guān)的基因數(shù)量最多;系統(tǒng)發(fā)育分析發(fā)現(xiàn),擬南芥基因和水稻基因進(jìn)化相對較快,并且在擬南芥中這一基因家族有3個基因簇,它們與基因的串聯(lián)重復(fù)加倍有關(guān)聯(lián);適應(yīng)性進(jìn)化分析揭示B-亞基相關(guān)的基因在進(jìn)化過程中受正選擇壓力影響進(jìn)化。
[Abstract]:V-ATPase plays a very important role in plant adaptation to stress. In this study, V-ATPase-related genes of Arabidopsis thaliana were used as target sequences in six gramineous plants (rice, barley, maize, sorghum). Comparative analysis was carried out in the genomes of millet and Spartina mongolicum to reveal the amplification mechanism and adaptive evolution of the gene family. The analysis showed that although seven different plant genomes experienced different whole genome doubling, the number of genes associated with millet and maize doubling was the largest, but the number of V-ATPase genes was very similar in each genome. In particular, maize in Gramineae has one more single specific doubling relative to other species, but the number of this gene family has not increased, indicating that genome-wide doubling, although to a certain extent, leads to the amplification of family genes. But gene loss may also maintain the genome's dose; The motif classification of 161 V-ATPase genes in seven genomes showed that the structure of motif in different species was different, and the number of genes related to c-subunit in Arabidopsis and rice was the most. Phylogenetic analysis showed that Arabidopsis gene and rice gene evolved relatively quickly, and there were three gene clusters in Arabidopsis thaliana gene family, which were associated with tandem repeat doubling. Adaptive evolution analysis revealed that B-subunit related genes were influenced by positive selection pressure during evolution.
【作者單位】： 華北理工大學(xué)生命科學(xué)學(xué)院基因組學(xué)與計算生物學(xué)研究中心;
【基金】：河北省自然科學(xué)基金(C2014209201;C2014209134) 華北理工大學(xué)大學(xué)生創(chuàng)新項目(X2015174)共同資助
【分類號】：Q943.2

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