普通小麥-十倍體長穗偃麥草衍生新品種抗赤霉病基因的分子鑒別
發(fā)布時間：2019-04-27 05:00
【摘要】：【目的】赤霉病(Fusarium head blight,FHB)是世界范圍內(nèi)嚴(yán)重危害小麥生產(chǎn)的病害之一。十倍體長穗偃麥草(Thinopyrum ponticum)具有優(yōu)良的赤霉病抗性,普通小麥-十倍體長穗偃麥草衍生新品種西農(nóng)509、西農(nóng)511和西農(nóng)529在田間展現(xiàn)出較強的赤霉病抗性。本文旨在對這3個品種的赤霉病抗性基因進行分子鑒別,為它們在小麥赤霉病抗性遺傳改良中的應(yīng)用提供理論依據(jù)�！痉椒ā客ㄟ^赤霉病菌(Fusarium graminearum)人工接種鑒定,明確3個小麥新品種對赤霉病的抗性水平。利用偃麥草E組染色體(臂)第1—7同源群的特異引物對3個普通小麥-十倍體長穗偃麥草衍生新品種及其主要親本小偃693、小偃597和十倍體長穗偃麥草進行分子鑒定,確定其長穗偃麥草的遺傳區(qū)段。利用與長穗偃麥草7EL染色體上抗赤霉病基因Fhb7緊密連鎖的標(biāo)記對實驗材料進行分析,明確該抗性基因與Fhb7的關(guān)系。【結(jié)果】鑒定結(jié)果表明,西農(nóng)509、西農(nóng)529和西農(nóng)511的赤霉病抗性與中抗對照品種揚麥158的抗性水平相當(dāng),表現(xiàn)為中抗。105個長穗偃麥草E基因組特異標(biāo)記中有7個在3個新品種中均能擴增出長穗偃麥草的特異條帶,其中5個標(biāo)記定位于7EL染色體臂上,2個標(biāo)記定位于7ES染色體臂上。利用97個定位于7E染色體的特異標(biāo)記進一步對小偃693、小偃597和3個新品種的遺傳片段進行鑒別,結(jié)果表明20個標(biāo)記能在5個長穗偃麥草衍生品種(系)擴增出穩(wěn)定的十倍體長穗偃麥草的特異條帶,其中包括與Fhb7緊密連鎖的Xsdau K8、Xsdau K144、Xsdau K27、Xsdau K99、Xcfa2040和Xsdau K116等6個標(biāo)記(7EL 149.00—7EL 153.77),即表明該區(qū)段源自于十倍體長穗偃麥草,跨距約89 c M。然而,已報道的7EL染色體臂末端與Fhb7兩側(cè)緊密連鎖的分子標(biāo)記Xsdau K60(7EL 153.77)、Xmag1932(7EL 154.70)、Xcfa2240(7EL 156.27)、Xsdau K66(7EL 158.02)、Xsdau K71(7EL 158.97)和Xsews19(7EL 160.00)等在3個新品種及小偃597中均沒檢出長穗偃麥草的特異條帶�！窘Y(jié)論】普通小麥-十倍體長穗偃麥草衍生新品種西農(nóng)509、西農(nóng)511和西農(nóng)529具有較好的赤霉病抗性,攜帶來自于十倍體長穗偃麥草7E染色體的遺傳區(qū)段,然而該抗赤霉病基因不同于Fhb7。
[Abstract]:Objective: scab (Fusarium head blight,FHB) is one of the most harmful diseases to wheat production in the world. (Thinopyrum ponticum) showed excellent resistance to scab. Xinong 599, Xinong 511 and Xinong 529 showed strong resistance to scab in the field of Triticum aestivum-Decaploid Elytrigia tenuiflora derived varieties Xinong 509, Xinong 511 and Xinong 529. The aim of this paper is to identify the genes of resistance to scab in these three cultivars, and to provide theoretical basis for their application in genetic improvement of resistance to scab in wheat. [methods] (Fusarium graminearum) inoculation was used to identify the resistance genes of scab in wheat. The resistance level of 3 new wheat varieties to scab was determined. Three new varieties of Triticum aestivum-Decaploid and their main parent parents Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum were identified by using specific primers of group E chromosome (arm) 1-7 homologue of Thinopyrum triticum, and their main parents were Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum. The genetic region of Thinopyrum elongatum was determined. A marker closely linked to the resistance gene Fhb7 of Elytrigia elongatum 7EL chromosome was used to identify the relationship between the resistance gene and Fhb7. [results] the identification results showed that Xinong 509, The resistance to scab of Xinong 529 and Xinong 511 was similar to that of medium resistant variety Yangmai 158. Seven of the 105 E genome-specific markers of Thinopyrum elongatum could amplify the specific bands of Thinopyrum elongatum in three new varieties, of which 5 were located on the arm of 7EL chromosome, and 5 of them were located on the arm of chromosome of Elytrigia elongatum. Two markers were located on the 7ES chromosome arm. The genetic fragments of Xiaoyan 693, Xiaoyan 597 and three new varieties were further identified by using 97 specific markers located on chromosome 7e. The results showed that 20 markers could amplify stable decaploid specific bands of Thinopyrum elongatum in 5 varieties (lines), including Xsdau K8, Xsdau K144, Xsdau K27, Xsdau K99, which were closely linked to Fhb7. Six markers (7EL 149.00-7EL 153.77), such as Xcfa2040 and Xsdau K116, indicated that the region originated from Thinopyrum tenuiformis, with a span of about 89 c. However, the reported molecular markers Xsdau K60 (7EL 153.77), Xmag1932 (7EL 154.70), Xcfa2240 (7EL 156.27), Xsdau K66 (7EL 158.02) linked closely to the ends of the 7EL chromosome arm to both sides of the Fhb7. Xsdau K71 (7EL 158.97) and Xsews19 (7EL 160.00) did not detect specific bands of Thinopyrum elongatum in three new varieties and Xiaoyan 597. [conclusion] Xinong 509, a new wheat-decaploid derived variety of Thinopyrum longiflorum, has not been detected. Xinong 511 and Xinong 529 have good resistance to scab and carry a genetic region from chromosome 7e of Thinopyrum tenuiflora. However, this resistance gene is different from that of Fhb7..
【作者單位】： 西北農(nóng)林科技大學(xué)農(nóng)學(xué)院/旱區(qū)作物逆境生物學(xué)國家重點實驗室;陜西省城固縣種子管理站;陜西省扶貧開發(fā)辦公室;
【基金】：國家重點研發(fā)計劃(2016YFD0102000) 陜西省科技統(tǒng)籌創(chuàng)新工程計劃(2015KTZDNY01-01-02)
【分類號】：S435.121.45
本文編號：2466707
[Abstract]:Objective: scab (Fusarium head blight,FHB) is one of the most harmful diseases to wheat production in the world. (Thinopyrum ponticum) showed excellent resistance to scab. Xinong 599, Xinong 511 and Xinong 529 showed strong resistance to scab in the field of Triticum aestivum-Decaploid Elytrigia tenuiflora derived varieties Xinong 509, Xinong 511 and Xinong 529. The aim of this paper is to identify the genes of resistance to scab in these three cultivars, and to provide theoretical basis for their application in genetic improvement of resistance to scab in wheat. [methods] (Fusarium graminearum) inoculation was used to identify the resistance genes of scab in wheat. The resistance level of 3 new wheat varieties to scab was determined. Three new varieties of Triticum aestivum-Decaploid and their main parent parents Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum were identified by using specific primers of group E chromosome (arm) 1-7 homologue of Thinopyrum triticum, and their main parents were Xiaoyan 693, Xiaoyan 597 and Decaploid Thinopyrum elongatum. The genetic region of Thinopyrum elongatum was determined. A marker closely linked to the resistance gene Fhb7 of Elytrigia elongatum 7EL chromosome was used to identify the relationship between the resistance gene and Fhb7. [results] the identification results showed that Xinong 509, The resistance to scab of Xinong 529 and Xinong 511 was similar to that of medium resistant variety Yangmai 158. Seven of the 105 E genome-specific markers of Thinopyrum elongatum could amplify the specific bands of Thinopyrum elongatum in three new varieties, of which 5 were located on the arm of 7EL chromosome, and 5 of them were located on the arm of chromosome of Elytrigia elongatum. Two markers were located on the 7ES chromosome arm. The genetic fragments of Xiaoyan 693, Xiaoyan 597 and three new varieties were further identified by using 97 specific markers located on chromosome 7e. The results showed that 20 markers could amplify stable decaploid specific bands of Thinopyrum elongatum in 5 varieties (lines), including Xsdau K8, Xsdau K144, Xsdau K27, Xsdau K99, which were closely linked to Fhb7. Six markers (7EL 149.00-7EL 153.77), such as Xcfa2040 and Xsdau K116, indicated that the region originated from Thinopyrum tenuiformis, with a span of about 89 c. However, the reported molecular markers Xsdau K60 (7EL 153.77), Xmag1932 (7EL 154.70), Xcfa2240 (7EL 156.27), Xsdau K66 (7EL 158.02) linked closely to the ends of the 7EL chromosome arm to both sides of the Fhb7. Xsdau K71 (7EL 158.97) and Xsews19 (7EL 160.00) did not detect specific bands of Thinopyrum elongatum in three new varieties and Xiaoyan 597. [conclusion] Xinong 509, a new wheat-decaploid derived variety of Thinopyrum longiflorum, has not been detected. Xinong 511 and Xinong 529 have good resistance to scab and carry a genetic region from chromosome 7e of Thinopyrum tenuiflora. However, this resistance gene is different from that of Fhb7..
【作者單位】： 西北農(nóng)林科技大學(xué)農(nóng)學(xué)院/旱區(qū)作物逆境生物學(xué)國家重點實驗室;陜西省城固縣種子管理站;陜西省扶貧開發(fā)辦公室;
【基金】：國家重點研發(fā)計劃(2016YFD0102000) 陜西省科技統(tǒng)籌創(chuàng)新工程計劃(2015KTZDNY01-01-02)
【分類號】：S435.121.45
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