【摘要】：核仁G蛋白1(Nucleolar G protein 1,NOG1)是一種高度保守的核仁GTP酶,在真核生物中廣泛存在,參與60 S核糖體亞基前體的組裝。在線蟲中敲減NOG1的表達造成生長緩慢、蟲體變小和壽命延長的表型,而過量表達NOG1則使線蟲的壽命縮短。擬南芥的At1g10300基因注釋為NOG1-2,但是其生物學功能還有待研究。該研究對其功能進行了初步研究,首先檢測了該基因在擬南芥各個器官的表達情況。結(jié)果表明:該基因在7 d齡幼苗、莖生葉和花中均有表達,其中在花中表達量最高。獲得了At1g10300基因的T-DNA插入突變體,發(fā)現(xiàn)在長日照條件下,At1g10300突變體植株的蓮座緊湊,蓮座葉片長寬比降低,但葉面積和植株高度與野生型相比無顯著差異,表明其葉形發(fā)生改變;突變體植株的抽薹時間晚于野生型。熒光定量RT-PCR結(jié)果表明,突變體植株中開花促進因子FT、CO和GI的表達水平下調(diào),而開花抑制因子FLC的表達水平上調(diào)。以上結(jié)果揭示At1g10300基因的突變影響了FT、CO、GI及FLC基因的表達,使植株出現(xiàn)晚花表型。
[Abstract]:Nucleolar G protein 1 (Nucleolar G protein 1 (NOG 1) is a highly conserved nucleolar GTP enzyme, which exists widely in eukaryotes and participates in the assembly of 60 S ribosomal subunit precursors. Knockdown of NOG1 expression in nematodes resulted in slow growth, decreased body size and prolonged lifespan, while over-expression of NOG1 shortened the lifespan of nematodes. The At1g10300 gene of Arabidopsis thaliana is annotated as NOG1-2, but its biological function remains to be studied. The function of the gene was studied. The expression of the gene in Arabidopsis was detected at first, and the expression of the gene in various organs of Arabidopsis thaliana was detected. The results showed that the gene was expressed in 7-day-old seedlings, stem leaves and flowers, and the highest expression was found in flowers. The T-DNA insertion mutant of At1g10300 gene was obtained. It was found that the rosette of At1g10300 mutant was compact and the ratio of leaf length to width of rosette decreased under long sunshine, but there was no significant difference in leaf area and plant height between wild type and At1g10300 mutant. It indicated that the leaf shape changed. Bolting time of mutant plants was later than that of wild type plants. The results of fluorescence quantitative RT-PCR showed that the expression levels of FT,CO and GI in mutant plants were down-regulated, while the expression level of FLC was up-regulated. The results indicated that the mutation of At1g10300 gene affected the expression of FT,CO,GI and FLC genes, and the late flower phenotype appeared in the plants.
【作者單位】： 南開大學生命科學學院植物生物學和生態(tài)學系;
【基金】：國家自然科學基金(31570247,91417308,91017009,31460453) 天津市自然科學基金(14JCYBJC41200)~~
【分類號】：Q943.2
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本文編號：2452275