發(fā)布時(shí)間：2019-03-29 18:10

【摘要】：釀酒酵母是第一個(gè)完成全基因組測(cè)序工作的真核生物。由于全基因組中基因數(shù)量比較少,且結(jié)構(gòu)簡(jiǎn)單,遺傳背景也相對(duì)清楚,所以釀酒酵母被廣泛作為真核模式生物。目前釀酒酵母在基因功能分析、轉(zhuǎn)錄組、蛋白質(zhì)組等多個(gè)層次上的研究取得了飛速的發(fā)展。釀酒酵母在現(xiàn)代分子生物學(xué)和細(xì)胞生物學(xué)的研究中發(fā)揮了重要作用。利用人類基因組和高等生物與釀酒酵母基因組之間的組織結(jié)構(gòu)和編碼順序上的同源性,不但可以為我們研究高等生物的生命提供良好的參考模型,而且也可以為人類基因組的研究提供良好積極的借鑒。本論文首先基于釀酒酵母全基因組的核苷酸序列,基因表達(dá)水平數(shù)據(jù),基因編碼蛋白質(zhì)的功能分類數(shù)據(jù),以及基因的重要性分類(必要和非必要基因)構(gòu)建了用于統(tǒng)計(jì)分析的釀酒酵母表達(dá)分析數(shù)據(jù)庫(kù)。從釀酒酵母基因的序列特征、功能分類與網(wǎng)絡(luò)調(diào)控等多個(gè)方面對(duì)基因表達(dá)水平變化進(jìn)行了研究。研究表明:1、基因表達(dá)水平的變化與基因功能相關(guān),功能重要的基因傾向于在細(xì)胞周期各時(shí)間段穩(wěn)定表達(dá)。2、基因表達(dá)水平的變化與基因的折疊穩(wěn)定性和tRNA拷貝數(shù)相關(guān),基因的折疊穩(wěn)定性越低其表達(dá)水平在各階段的變化越大,而較低的tRNA拷貝數(shù)也傾向于使基因的表達(dá)水平出現(xiàn)較大變化。3、基因共表達(dá)網(wǎng)絡(luò)分析表明基因表達(dá)水平變化與其協(xié)同共表達(dá)的基因數(shù)呈顯著正相關(guān)。
[Abstract]:Saccharomyces cerevisiae is the first eukaryotes to complete genome-wide sequencing. Saccharomyces cerevisiae is widely used as eukaryotes because of the small number of genes in the whole genome, simple structure and relatively clear genetic background. At present, Saccharomyces cerevisiae has made rapid development in gene function analysis, transcriptome, proteome and so on. Saccharomyces cerevisiae plays an important role in the research of modern molecular biology and cell biology. Making use of the homology of tissue structure and coding sequence between human genome and higher organism and Saccharomyces cerevisiae genome can not only provide a good reference model for us to study the life of higher organisms. And it can also provide a good and positive reference for the study of human genome. Firstly, based on the nucleotide sequence of the whole genome of Saccharomyces cerevisiae, the data of gene expression level, the functional classification data of gene coding protein, The database of Saccharomyces cerevisiae expression analysis for statistical analysis was constructed. The changes of gene expression level in Saccharomyces cerevisiae were studied in terms of sequence characteristics, functional classification and network regulation. The results showed that: 1, the change of gene expression level was related to gene function, and the genes with important function tended to express stably in each period of cell cycle. 2. The change of gene expression level was related to the folding stability of gene and the copy number of tRNA, and the change of gene expression level was related to gene folding stability and copy number. The lower the folding stability of the gene is, the greater the expression level changes at all stages, and the lower the copy number of tRNA tends to make the gene expression level change greatly. 3, The analysis of coexpression network showed that there was a significant positive correlation between the level of gene expression and the number of co-expressed genes.
【學(xué)位授予單位】：河北工業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：Q78

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本文編號(hào)：2449766