【摘要】：【目的】為探明GDSL家族脂肪酶的抗逆機理,【方法】在前期研究基礎上,利用RACE技術克隆木豆GDSL脂肪酶基因,并通過熒光定量PCR技術檢測其表達特性。【結果】獲得1條木豆GDSL脂肪酶基因,命名為Cc GDSL1,該基因含開放閱讀框全長1107 bp,編碼369個氨基酸;經(jīng)同源性分析,Cc GDSL1編碼的氨基酸序列與豆科植物中GDSL脂肪酶具有較高的相似性。該基因在葉、莖和根中均表達,且葉和莖中表達量顯著高于根;干旱脅迫能提高Cc GDSL1的表達量,隨干旱的持續(xù)表現(xiàn)出先降后升隨后趨于穩(wěn)定的表達模式�！窘Y論】推測Cc GDSL1基因參與木豆應激干旱脅迫。
[Abstract]:[objective] to investigate the anti-stress mechanism of GDSL family lipases, [methods] on the basis of previous studies, the GDSL lipase gene was cloned by RACE. The expression characteristics of GDSL lipase gene were detected by fluorescence quantitative PCR. [results] A GDSL lipase gene was obtained, named Cc GDSL1, which contained an open reading frame (ORF) of 1107 bp, encoding 369 amino acids. The homology analysis showed that the amino acid sequence encoded by Cc GDSL1 was similar to GDSL lipase in legumes. The gene was expressed in leaves, stems and roots, and the expression level in leaves and stems was significantly higher than that in roots. Drought stress could increase the expression level of Cc GDSL1, and showed a stable expression pattern with continuous drought. [conclusion] Cc GDSL1 gene may be involved in drought stress of pigeonpea. [conclusion] it is suggested that Cc GDSL1 gene may play an important role in the drought stress of pigeonpea.
【作者單位】： 貴州大學農業(yè)生物工程研究院山地植物資源保護與種質創(chuàng)新省部共建教育部重點實驗室;貴州大學貴州省森林資源與環(huán)境研究中心;
【基金】：國家自然科學基金項目“利用Gene Fishing解析VA菌根增強木豆抗旱能力的分子機理”(30860224) 貴州省科學技術基金項目“在轉錄水平解析木豆抗旱的分子機理”(20092076)
【分類號】：Q943.2;S567.19

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1 郝曉云;蔡永智;錢雯婕;袁哈利;李榕;李鴻彬;;植物GDSL脂肪酶家族研究進展[J];植物生理學報;2013年12期

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