【摘要】：火力楠具有重要經(jīng)濟(jì)價(jià)值與生態(tài)功能,但缺乏其基因組信息,限制了其分子生物學(xué)、基因功能與分子育種的相關(guān)研究。以火力楠根、莖和葉為材料,利用Illumina高通量測(cè)序技術(shù)進(jìn)行轉(zhuǎn)錄組測(cè)序,并對(duì)得到的測(cè)序數(shù)據(jù)進(jìn)行de novo組裝,獲得97 503條Unigenes,N50為1 010 bp、平均長(zhǎng)度852 bp。與公共數(shù)據(jù)庫(kù)進(jìn)行比對(duì),注釋到NR、Swiss-Prot數(shù)據(jù)庫(kù)的Unigenes分別為60 926、44 249條。將Unigenes與COG數(shù)據(jù)庫(kù)比對(duì),有42 344條Unigenes成功注釋,根據(jù)功能大致分成25類;與GO數(shù)據(jù)庫(kù)比對(duì),有15 947條Unigenes獲得注釋,按功能可分為生物學(xué)過程、細(xì)胞組分和分子功能3大類55亞類,其中參與的生物學(xué)過程較多;以KEGG數(shù)據(jù)庫(kù)參考,有26 267條Unigenes參與330條代謝途徑分支,以代謝相關(guān)的途徑較為集中;差異基因表達(dá)分析我們得出,根與莖的差異表達(dá)基因?yàn)? 826個(gè),根與葉之間的差異表達(dá)基因?yàn)? 230個(gè),這兩組差異基因相對(duì)校多,除此之外莖與葉差異基因相對(duì)較少;差異基因的GO分類、GO富集以及pathway富集分析表明,主要在細(xì)胞組分、蛋白質(zhì)活性、生物合成以及代謝過程等所占比例較高。這些研究結(jié)果極大地?cái)U(kuò)充了火力楠的基因資源,將有助于火力楠基因的發(fā)掘與利用、分子標(biāo)記的開發(fā)及其種質(zhì)資源遺傳改良的研究等。
[Abstract]:Photinia officinalis has important economic value and ecological function, but the lack of genomic information limits its molecular biology, gene function and molecular breeding related research. Using root, stem and leaf as materials, the transcript sequence was sequenced by Illumina high flux sequencing technique, and the obtained sequencing data were assembled by de novo. 97,503 Unigenes,N50 were obtained as the average length of 1010 bp, and the average length of 97,503 Unigenes,N50 was 1 010 bp, and the average length of the sequence was 1 010 bp,. Compared with the common database, the Unigenes annotated to the NR,Swiss-Prot database was 60926,44,249 respectively. Comparing Unigenes with COG database, there are 42,344 successful comments of Unigenes, which are divided into 25 categories according to their functions. Compared with GO database, 15,947 Unigenes were annotated, which could be divided into biological process, cellular component and molecular function. According to KEGG database, 26,267 Unigenes were involved in 330 branches of metabolic pathway, and the metabolic related pathways were more concentrated. The differential gene expression analysis showed that there were 2 826 differentially expressed genes between root and stem and 3 230 differentially expressed genes between root and leaf. In addition, there were less differentially expressed genes between stem and leaf in the two groups. The analysis of GO classification, GO enrichment and pathway enrichment of differential genes showed that the proportion of differential genes was higher in cell composition, protein activity, biosynthesis and metabolic process. These results greatly expand the genetic resources of Photinia, which will contribute to the exploration and utilization of the gene, the development of molecular markers and the genetic improvement of germplasm resources, etc.
【作者單位】： 中國(guó)林業(yè)科學(xué)研究院熱帶林業(yè)研究所熱帶林業(yè)研究國(guó)家林業(yè)局重點(diǎn)實(shí)驗(yàn)室;
【基金】：中央公益性科研院所基金項(xiàng)目(RITFYWZX201506;CAFYBB2016SZ002) “十三五”國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目任務(wù)(2017YFD0601101-7) 林業(yè)公益性行業(yè)科研專項(xiàng)(201204304-02)共同資助
【分類號(hào)】：Q943.2;S792.99

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