【摘要】：從粳稻品種日本晴經(jīng)60 Co誘變的M1代材料中發(fā)現(xiàn)了一個(gè)白化致死突變體,該突變體從萌芽后一直表現(xiàn)白化,3葉期后逐漸衰亡。遺傳分析表明,該突變表型受一對(duì)隱性核基因控制,將該白化突變體暫定名為al14。與野生型相比,al14突變體的葉綠素含量與類(lèi)胡蘿卜素含量顯著降低。電子顯微鏡觀察表明al14突變體不能形成完整的葉綠體,只有原片層體結(jié)構(gòu)。對(duì)葉綠體編碼基因的表達(dá)分析發(fā)現(xiàn),突變體中光系統(tǒng)Ⅰ和光系統(tǒng)Ⅱ基因表達(dá)明顯下調(diào),核糖體結(jié)構(gòu)基因和質(zhì)體編碼的RNA聚合酶亞基基因表達(dá)明顯上調(diào),但是PsbN(photosystemⅡprotein N)卻上調(diào)表達(dá)水平最高,達(dá)到118.23倍。利用al14突變體與黃華占雜交獲得的F2代分離群體進(jìn)行基因定位,將該基因定位于水稻第6染色體上約40kb的范圍。目前,該范圍內(nèi)沒(méi)有葉色相關(guān)基因的報(bào)道,可能為一新的調(diào)控葉綠體發(fā)育的基因。
[Abstract]:A albino-lethal mutant was found in M1 generation of Japonica rice variety Qingjing 60 Co. The mutant appeared albinism after germination and gradually declined after 3-leaf stage. Genetic analysis showed that the mutant phenotype was controlled by a pair of recessive nuclear genes. The albino mutant was provisionally named al14.. Compared with wild type, the chlorophyll content and carotenoid content of al14 mutant decreased significantly. Electron microscopy showed that the al14 mutant could not form complete chloroplast, but only prolamellar lamellar structure. The expression analysis of chloroplast coding gene showed that the expression of photosystem 鈪，

本文編號(hào)：2430718