毛竹葉片衰老特性及衰老相關(guān)基因的篩選和鑒定
發(fā)布時間：2019-01-21 12:53
【摘要】：收集野外定植3年的毛竹稈上具有明顯衰老漸進(jìn)特征的葉片簇,測量這些葉片的光合代謝效率(Fv/Fm)及葉綠素含量,建立毛竹不同衰老階段葉片形態(tài)與其光合效率之間的關(guān)系.在此基礎(chǔ)上,通過生物信息學(xué)方法(BLASTn)篩選出113個毛竹基因組中與水稻葉片衰老調(diào)控相關(guān)的同源基因,并檢測這些基因在毛竹葉片發(fā)育和衰老過程中的表達(dá)趨勢,鑒定一些與毛竹葉片衰老相關(guān)的標(biāo)志基因.結(jié)果顯示,毛竹葉片在衰老過程中其葉片的光合效率和葉綠素含量均出現(xiàn)顯著下降;在篩選的113個基因中有86個基因在葉片中表達(dá),其中32個基因在葉片衰老過程中沒有變化,41個基因隨著葉片衰老出現(xiàn)顯著下調(diào),13個基因出現(xiàn)明顯上調(diào).這些表達(dá)變化的基因與水稻中鑒定的同源基因在旗葉衰老過程中的表達(dá)模式基本一致,因此這些基因很可能在毛竹葉片衰老過程中也發(fā)揮同樣的功能.
[Abstract]:The leaf clusters with distinct senescence and progressive characteristics were collected and the photosynthetic metabolic efficiency (Fv/Fm) and chlorophyll content of the leaves were measured after 3 years of field planting. The relationship between leaf morphology and photosynthetic efficiency of Phyllostachys pubescens in different senescence stages was established. On this basis, 113 homologous genes related to the regulation of leaf senescence in rice were screened by bioinformatics method (BLASTn), and the expression trend of these genes in the development and senescence process of Phyllostachys pubescens was detected. Some marker genes related to leaf senescence of Phyllostachys pubescens were identified. The results showed that the photosynthetic efficiency and chlorophyll content in leaves of Phyllostachys pubescens decreased significantly during senescence. Of the 113 genes screened, 86 genes were expressed in leaves, 32 of them were not changed during leaf senescence, 41 genes were significantly down-regulated with leaf senescence, and 13 genes were up-regulated. The expression patterns of these genes were similar to those of the homologous genes identified in rice during flag leaf senescence. Therefore these genes may also play the same function in the senescence of bamboo leaves.
【作者單位】： 福建農(nóng)林大學(xué)生命科學(xué)學(xué)院分子細(xì)胞和系統(tǒng)生物學(xué)中心;
【基金】：“十二五”農(nóng)村領(lǐng)域國家科技計(jì)劃課題(2015BAD04B01-2)
【分類號】：S795.7
本文編號：2412682
[Abstract]:The leaf clusters with distinct senescence and progressive characteristics were collected and the photosynthetic metabolic efficiency (Fv/Fm) and chlorophyll content of the leaves were measured after 3 years of field planting. The relationship between leaf morphology and photosynthetic efficiency of Phyllostachys pubescens in different senescence stages was established. On this basis, 113 homologous genes related to the regulation of leaf senescence in rice were screened by bioinformatics method (BLASTn), and the expression trend of these genes in the development and senescence process of Phyllostachys pubescens was detected. Some marker genes related to leaf senescence of Phyllostachys pubescens were identified. The results showed that the photosynthetic efficiency and chlorophyll content in leaves of Phyllostachys pubescens decreased significantly during senescence. Of the 113 genes screened, 86 genes were expressed in leaves, 32 of them were not changed during leaf senescence, 41 genes were significantly down-regulated with leaf senescence, and 13 genes were up-regulated. The expression patterns of these genes were similar to those of the homologous genes identified in rice during flag leaf senescence. Therefore these genes may also play the same function in the senescence of bamboo leaves.
【作者單位】： 福建農(nóng)林大學(xué)生命科學(xué)學(xué)院分子細(xì)胞和系統(tǒng)生物學(xué)中心;
【基金】：“十二五”農(nóng)村領(lǐng)域國家科技計(jì)劃課題(2015BAD04B01-2)
【分類號】：S795.7
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