發(fā)布時(shí)間：2019-01-02 14:29

【摘要】：目的：利用基因芯片技術(shù)分析溫陽(yáng)消飲法調(diào)控胸腔積液模型大鼠基因表達(dá)的作用機(jī)制。方法：40只清潔級(jí)Wistar成年大鼠適應(yīng)性飼養(yǎng)7天后,隨機(jī)分為溫陽(yáng)消飲組(W)、去桂消飲組(Q)、模型對(duì)照組(M)、空白對(duì)照組(K),每組10只。除空白對(duì)照組外,其余3組以1%λ-角叉菜膠胸腔注射建立胸腔積液(類(lèi)似懸飲)大鼠模型,造模24h后拍X線(xiàn)片對(duì)各組大鼠進(jìn)行病理評(píng)價(jià)。于造模后2h各組以相應(yīng)藥物灌胃,每天1次,連續(xù)5天。灌胃結(jié)束后大鼠進(jìn)行安樂(lè)死。每組取3只大鼠的腎髓質(zhì)和空腸組織,采用基因芯片技術(shù)進(jìn)行基因表達(dá)譜檢測(cè),篩選出差異表達(dá)基因,并進(jìn)行Pathway分析。結(jié)果：1.腎髓質(zhì)組織基因表達(dá)譜檢測(cè)溫陽(yáng)消飲組：溫陽(yáng)消飲組與模型對(duì)照組比較和模型對(duì)照組與空白對(duì)照組比較共同表達(dá)的通路為：神經(jīng)活性配體受體相互作用通路、系統(tǒng)性紅斑狼瘡、腎素血管緊張素系統(tǒng),共同表達(dá)基因?yàn)?LOC317471、Olr462、Mcpt8I3、Olr718、Mcpt8I2、 Cd80、Ttn。去桂消飲組：去桂消飲組與模型對(duì)照組比較和模型對(duì)照組與空白對(duì)照組比較共同表達(dá)的通路為：神經(jīng)活性配體受體相互作用通路,共同表達(dá)的基因?yàn)镃ep295nl、Mcpt8I3、Fam64a。2.空腸組織基因表達(dá)譜檢測(cè)溫陽(yáng)消飲組：溫陽(yáng)消飲組與模型對(duì)照組比較和模型對(duì)照組與空白對(duì)照組比較共同表達(dá)的通路為：藥物代謝細(xì)胞色素P450通路、卟啉和葉綠素代謝通路、淀粉和蔗糖代謝通路、甾類(lèi)激素生物合成通路、嗅覺(jué)傳導(dǎo)通路、藥物其他酶代謝通路、細(xì)胞色素P450外源性化學(xué)物質(zhì)通路、戊糖和葡萄糖醛酸相互轉(zhuǎn)換通路、視黃酵代謝通路,共同表達(dá)的基因?yàn)椋篊acnb4、Gpr27、Ugt2b、Spock3、Olr113、 Olr1472、Ugt2b7、Opn5、RGD1564865、Ugt2b17、Ugt2b37、Slc5a5、Slc4a9、 Ccdc79、Anks1b。去桂消飲組：去桂消飲組與模型對(duì)照組比較和模型對(duì)照組與空白對(duì)照組比較共同表達(dá)的通路為：藥物代謝細(xì)胞色素P450通路,共同表達(dá)的基因?yàn)椋篕cnq2、 LOC102548590、Cyp2b12、Cyp2b21、Aldhla1、Cyp2d3、Arnt2。結(jié)論：1.溫陽(yáng)消飲法可以減少λ-角叉菜膠誘導(dǎo)的胸腔積液模型大鼠的胸腔積液量。2.利用基因芯片技術(shù)對(duì)胸腔積液模型大鼠腎髓質(zhì)進(jìn)行差異基因分析,發(fā)現(xiàn)溫陽(yáng)消飲方與去桂消飲方可能是通過(guò)上調(diào)Mcpt8I3基因調(diào)控神經(jīng)活性配體受體相互作用通路,參與炎癥反應(yīng)。3.溫陽(yáng)消飲組與去桂消飲組在腎髓質(zhì)組織的pathway分析中存在差異,溫陽(yáng)消飲方可能是通過(guò)上調(diào)Mcpt8I3基因調(diào)控腎素血管緊張素系統(tǒng)通路達(dá)到利尿消飲的目的。4.利用基因芯片技術(shù)對(duì)胸腔積液模型大鼠空腸組織進(jìn)行pathway分析,去桂消飲組未發(fā)現(xiàn)有與利尿作用相關(guān)的通路,而溫陽(yáng)消飲方可能通過(guò)調(diào)控甾類(lèi)激素生物合成通路,抑制醛固酮的合成來(lái)達(dá)到利尿的作用。5.本研究中未發(fā)現(xiàn)cAMP-PKA/PKC及VWP通路,提示溫陽(yáng)消飲方可能不是通過(guò)該通道發(fā)揮利尿或抑制尿液重吸收作用的。
[Abstract]:Aim: to analyze the mechanism of Wenyang Xiaoyin method in regulating gene expression in rats with pleural effusion. Methods: 40 clean grade Wistar adult rats were fed for 7 days, and were randomly divided into Wanyang Xiaoyin group, (W), group, (Q), model control group, (M), blank control group, (K), group, 10 rats in each group. In addition to the blank control group, the other three groups were injected with 1% 位 -carrageenin into the pleural cavity to establish the model of pleural effusion (similar to suspension drink). Two hours after modeling, each group was gavaged with corresponding drugs once a day for 5 days. The rats were euthanized after gavage. The kidney medulla and jejunum of 3 rats in each group were collected and the differentially expressed genes were screened by gene chip technique and analyzed by Pathway. Results: 1. The gene expression profile of renal medulla tissue in Wenyang Xiaoyin group: compared with the model control group and the model control group compared with the blank control group: the neuroactive ligand receptor interaction pathway, systemic lupus erythematosus. Renin angiotensin system, co-expressed by LOC317471,Olr462,Mcpt8I3,Olr718,Mcpt8I2, Cd80,Ttn. Compared with the model control group and the model control group, the co-expression pathway of the Deiguixiao Yin group and the blank control group was: the neuroactive ligand receptor interaction pathway, and the co-expressed gene was Cep295nl,Mcpt8I3,Fam64a.2.. Detection of gene expression profile in jejunum tissue: the pathways of gene expression in Wenyang Xiaoyin group and model control group were as follows: drug metabolism cytochrome P450 pathway, porphyrin and chlorophyll metabolism pathway, compared with model control group and model control group. Starch and sucrose metabolism pathway, steroid hormone biosynthesis pathway, olfactory pathway, drug other enzyme metabolism pathway, cytochrome P450 exogenous chemical pathway, pentose and glucuronic acid interconversion pathway, retinozyme metabolism pathway, The co-expressed genes are: Cacnb4,Gpr27,Ugt2b,Spock3,Olr113, Olr1472,Ugt2b7,Opn5,RGD1564865,Ugt2b17,Ugt2b37,Slc5a5,Slc4a9, Ccdc79,Anks1b. Compared with the model control group and the model control group, the drug metabolism cytochrome P450 pathway and the co-expressed gene Kcnq2, LOC102548590,Cyp2b12,Cyp2b21,Aldhla1,Cyp2d3,Arnt2. were found in the Deiguixiao decoction group and the model control group and the blank control group respectively. Conclusion: 1. Wenyang Xiaoyin method can reduce the amount of pleural effusion induced by 位-carrageenin in rats. 2. The differential gene analysis of renal medulla in rats with pleural effusion was carried out by using gene chip technique. It was found that Wenyang Xiaoyin recipe and Deiguixiaoyin prescription might regulate the neuroactive ligand receptor interaction pathway by up-regulating Mcpt8I3 gene. Participation in inflammatory response. 3. There were differences in pathway analysis of renal medulla between Wenyangxiaoyin group and Danguixiaoyin group. Wenyangxiaoyin prescription could achieve diuretic Xiaoyin by up-regulating the renin angiotensin system pathway by up-regulating Mcpt8I3 gene. 4. The pathway analysis of jejunum tissue of rats with pleural effusion model was carried out by using gene chip technology. There was no diuretic pathway in the group of deguixiao drink, while Wenyang Xiaoyin prescription could regulate steroid hormone biosynthesis pathway. Inhibition of aldosterone synthesis to achieve diuretic effect. 5. The cAMP-PKA/PKC and VWP pathways were not found in this study, suggesting that WYXF may not play diuretic or inhibit urinary reabsorption through this channel.
【學(xué)位授予單位】：成都中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R285.5;R-332

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張藝;134例胸腔積液臨床檢測(cè)結(jié)果分析[J];廣西預(yù)防醫(yī)學(xué);2001年S1期

2 范惠娟,陳恒;胸腔積液256例病因之探討[J];臨床肺科雜志;2001年03期

3 江祖勝;胸腔積液誤診1例[J];西藏醫(yī)藥雜志;2002年S1期

4 陳素敏;胸腔積液排出方法的改進(jìn)[J];中國(guó)煤炭工業(yè)醫(yī)學(xué)雜志;2002年06期

5 陳學(xué)文,趙善良,沙作和;356例胸腔積液臨床分析[J];寧夏醫(yī)學(xué)雜志;2004年05期

6 覃大烈,馮尚克,韋建華;一次性單腔中心靜脈導(dǎo)管用于小兒胸腔積液的引流(附24例報(bào)告)[J];廣西醫(yī)科大學(xué)學(xué)報(bào);2004年02期

7 鄒凱華,雷建明,馮婭琴,李曉華;胸腔積液超聲顯像與X線(xiàn)對(duì)照400例分析[J];福建醫(yī)藥雜志;2004年03期

8 俞森洋;重癥監(jiān)護(hù)治療病房中胸腔積液的診斷和治療[J];中國(guó)危重病急救醫(yī)學(xué);2004年07期

9 堵玉萍,李，

本文編號(hào)：2398613