【摘要】：BNC2基因在各種類型的細(xì)胞中廣泛存在,可能參與RNA剪接過程。該基因與角質(zhì)細(xì)胞增殖能力有關(guān),在細(xì)胞終極分化中起重要作用。前人研究結(jié)果表明BNC2基因可能與雞的色素合成相關(guān),且參與了小鼠的卵巢發(fā)育。本論文將BNC2基因作為候選基因,分析了其多態(tài)性與雞的銀耳性狀、產(chǎn)蛋性狀之間的關(guān)系。主要研究結(jié)果如下:1.采用PCR方法,從雞中獲得了BNC2基因的三個轉(zhuǎn)錄本,長度分別為3020bp,1066bp,866bp,均有開放閱讀框。2.對該BNC2基因進行蛋白的功能結(jié)構(gòu)域分析,發(fā)現(xiàn)其結(jié)構(gòu)域為Atrophin-1,并且其無跨膜區(qū)。對雞與斑馬魚、青溕魚、狗、大熊貓等動物BNC2基因進行了同源相似性分析,雞與中國倉鼠、斑馬魚、青溕魚屬于同一分支,親緣關(guān)系較近,與大熊貓、猴子、挪威鼠和家鼠親緣關(guān)系較遠(yuǎn)。3.運用熒光定量PCR檢測到BNC2基因在雞的肝、肺、腎、下丘腦、卵巢、耳等12種組織中都有表達,其中在肺、下丘腦、卵巢組織的表達量較高,在肝、腎組織的表達量較低;在非銀耳雞中,耳的表達量更高,約是銀耳雞表達量的4倍。4.對清遠(yuǎn)麻雞BNC2基因序列進行多態(tài)性位點篩查,共發(fā)現(xiàn)了20個SNPs。BNC2基因序列5’側(cè)翼區(qū)12個多態(tài)位點與銀耳性狀和產(chǎn)蛋性狀的關(guān)系,結(jié)果顯示g.366AT,g.3411GA與雞的銀耳性狀顯著關(guān)聯(lián)(P0.05);g.1600AG位點與雞的產(chǎn)蛋性狀關(guān)聯(lián)顯著(P0.05)。綜上所述,通過PCR方法克隆了BNC2基因,獲得了三個轉(zhuǎn)錄本,并且BNC2基因在雞的肝、肺、腎、下丘腦、卵巢、耳等組織表達,其多態(tài)性可能與清遠(yuǎn)麻雞的銀耳性狀、產(chǎn)蛋性狀顯著相關(guān)。
[Abstract]:BNC2 gene exists widely in various types of cells and may be involved in the RNA splicing process. The gene is related to the proliferation of keratinocytes and plays an important role in the ultimate differentiation of cells. Previous studies have suggested that BNC2 gene may be involved in the synthesis of chicken pigments and play an important role in the development of mouse ovaries. In this paper, BNC2 gene was used as a candidate gene to analyze the relationship between the polymorphism and the traits of Tremella and egg laying. The main results are as follows: 1. Using PCR method, three transcripts of BNC2 gene were obtained from chicken. The functional domain of the BNC2 gene was identified as Atrophin-1, and no transmembrane region. The homologous similarity analysis of BNC2 gene between chicken and zebrafish, green fish, dog, giant panda and other animals was carried out. Chicken and Chinese hamster, zebrafish, green fish belong to the same branch, close related to giant panda, monkey, etc. The relationship between Norway rat and house mouse is far away. 3. Fluorescence quantitative PCR was used to detect the expression of BNC2 gene in the liver, lung, kidney, hypothalamus, ovary, ear and other 12 tissues of chicken. The expression of BNC2 gene was higher in lung, hypothalamus and ovary, but lower in liver and kidney. In non-Tremella chickens, the expression level in the ear was 4. 4 times higher than that in the Tremella chicken. The polymorphic loci of BNC2 gene in Qingyuan hens were screened. A total of 12 polymorphic loci in 5 'flanking region of 20 SNPs.BNC2 gene sequences were found to be related to Tremella and egg laying traits. The results showed that g. 366AT. The correlation between g.3411GA and Tremella traits was significant (P0.05). There was a significant correlation between g.1600AG locus and egg laying traits (P0.05). To sum up, BNC2 gene was cloned by PCR, and three transcripts were obtained, and BNC2 gene was expressed in liver, lung, kidney, hypothalamus, ovary, ear and so on. Egg-laying traits were significantly correlated.
【學(xué)位授予單位】：華南農(nóng)業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：S831

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相關(guān)期刊論文 前1條

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本文編號：2317729