山東地區(qū)零售雞肉中沙門菌耐藥基因東地區(qū)零售雞肉中沙門菌耐藥基因、毒力基因及PFGE分型研究
發(fā)布時(shí)間:2018-10-25 11:19
【摘要】:為了解山東地區(qū)零售雞肉中沙門菌耐藥和毒力基因的攜帶狀況以及PFGE分子分型情況,采用PCR方法對(duì)分離的25株沙門菌攜帶的耐藥基因和毒力基因進(jìn)行檢測,并運(yùn)用XbaⅠ酶進(jìn)行酶切并完成PFGE分析,利用BioNumerics軟件對(duì)分離菌株的指紋圖譜進(jìn)行聚類分析。結(jié)果顯示,分離菌株攜帶耐藥基因的攜帶情況與耐藥表型的符合率為95%,其中88%的分離菌株攜帶酰胺醇類耐藥基因(catⅠ)和β-內(nèi)酰胺類耐藥基因(blatem-1/blapsE-1/blaCMY-1/blaoxA-1);所有分離菌株均攜帶fimAstn、IacP、prgK四種毒力基因,其次是spvr、spva、spvc,攜帶率均為64%;25株沙門菌共分為10個(gè)群,包括16個(gè)不同的帶型,菌株相似度為60%~100%。
[Abstract]:In order to understand the carrying status of Salmonella drug resistance and virulence genes and PFGE molecular typing in retail chicken in Shandong province, 25 isolates of Salmonella were detected by PCR method. Xba 鈪,
本文編號(hào):2293552
[Abstract]:In order to understand the carrying status of Salmonella drug resistance and virulence genes and PFGE molecular typing in retail chicken in Shandong province, 25 isolates of Salmonella were detected by PCR method. Xba 鈪,
本文編號(hào):2293552
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