【摘要】：為了研究多浪羊和卡拉庫爾羊促卵泡素受體(FSHR)基因第10外顯子的多態(tài)性及其與多浪羊胎次產(chǎn)羔數(shù)相關(guān)性,試驗采用PCR-SSCP和直接測序的方法進(jìn)行研究。結(jié)果表明:多浪羊和卡拉庫爾羊FSHR基因第10外顯子存在3個突變位點,分別為Arg 1 328 Lys、Gln 1 334 Pro和Asp 1 342Asn,均屬于錯義突變。在1 328位點有等位基因A和B,基因型僅為AA和AB;在1 334位點有等位基因C和D,基因型只有CD和DD;在1 342位點有等位基因E和F,基因型為EE、FF和EF。多浪羊與卡拉庫爾羊在1 334位點基因型分布存在顯著差異(P0.05),其他兩位點差異不顯著(P0.05);FSHR基因多態(tài)性與多浪羊胎次產(chǎn)羔數(shù)之間差異不顯著(P0.05),表明FSHR基因第10外顯子對多浪羊產(chǎn)羔數(shù)沒有顯著影響(P0.05)。
[Abstract]:In order to study the polymorphism of exon 10 of follicle stimulating hormone receptor (FSHR) gene and its correlation with litter size in Doulang and Karakuer sheep, PCR-SSCP and direct sequencing were used to study the relationship between the polymorphism and litter size. The results showed that there were three mutation sites in exon 10 of FSHR gene in Doulang sheep and Karakur sheep, which were Arg 1 328 Lys,Gln 1 334 Pro and Asp 1 342 Asn, respectively, which belonged to missense mutation. Alleles A and B at locus 1 328, alleles C and D at locus 1 334 only for AA and AB;, alleles E and F at locus 1 342 for CD and DD;, and genotype EE,FF and EF. for genotype 1 342 There was significant difference in genotype distribution at 1 334 locus between Do-long sheep and Karakur sheep (P0.05), but there was no significant difference between the other two loci (P0.05). (P0.05) there was no significant difference between polymorphism of); FSHR gene and litter size of Dulang sheep (P0.05), which indicated that FSHR gene exon 10 had no significant difference (P0.05). There was no significant effect on lambing number of Doulang sheep (P0.05).
【作者單位】： 塔里木大學(xué)動物科學(xué)學(xué)院;新疆生產(chǎn)建設(shè)兵團(tuán)塔里木畜牧科技重點實驗室;
【基金】：國家自然科學(xué)基金項目(31640076) 新疆生產(chǎn)建設(shè)兵團(tuán)塔里木畜牧科技重點實驗室項目(HS201310)
【分類號】：S826

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