【摘要】：開(kāi)花是植物生長(zhǎng)發(fā)育的重要過(guò)程。CCT家族基因在植物中廣泛存在,參與植物花期的調(diào)控過(guò)程。該文從粗山羊草(Aegilops tauschii)全基因組中分離出26個(gè)CCT基因,它們分布于7對(duì)染色體上,按照排列順序?qū)⑵涿麨锳etCCT1 26。AetCCT蛋白分子量介于14.9 kDa(AetCCT3) 83.2 kDa(AetCCT12)之間,其中有25個(gè)蛋白包含完整的CCT保守結(jié)構(gòu)域。系統(tǒng)發(fā)育分析顯示,12對(duì)粗山羊草/烏拉爾圖小麥(Triticum urartu)CCT蛋白和9對(duì)粗山羊草/水稻(Oryza sativa)CCT蛋白為直系同源蛋白。通過(guò)公共數(shù)據(jù)的數(shù)字表達(dá)分析表明,AetCCT具有組織特異性和組成型2種表達(dá)形式,其中AetCCT3、AetCCT4、AetCCT7及AetCCT9等9個(gè)基因在大部分組織中都有表達(dá),而AetCCT15、AetCCT21和AetCCT25等基因分別在種子、葉和根等少數(shù)組織中特異表達(dá)。AetCCT家族可以響應(yīng)不同外源激素,施用激素24小時(shí)和72小時(shí)后各成員對(duì)激素響應(yīng)整體表現(xiàn)一致,但不同成員對(duì)于不同激素的響應(yīng)存在差異,表明該家族成員在功能和行使方式等方面具有一定的多樣性,可能參與不同生長(zhǎng)發(fā)育過(guò)程。光照條件影響AetCCT的表達(dá),說(shuō)明光照和春化作用是影響與調(diào)控該家族基因表達(dá)的重要因素。研究結(jié)果有助于探索小麥(T.aestivum)進(jìn)化、馴化和演變的規(guī)律,以及認(rèn)識(shí)重要農(nóng)藝性狀的形成與互作網(wǎng)絡(luò)。
[Abstract]:Flowering is an important process of plant growth and development. CCT family genes exist widely in plants and participate in the regulation of plant flowering. In this paper, 26 CCT genes were isolated from the whole (Aegilops tauschii) genome of Leymus chinensis. They were distributed on 7 pairs of chromosomes. They were named AetCCT1 26.AetCCT protein with molecular weight between 14.9 kDa (AetCCT3) and 83.2 kDa (AetCCT12). Among them, 25 proteins contain complete conserved domain of CCT. Phylogenetic analysis showed that 12 pairs of (Triticum urartu) CCT protein and 9 pairs of (Oryza sativa) CCT protein of Leymus chinensis / Ulaertu were direct homologous proteins. The results of digital expression analysis of common data showed that AetCCT had two expression forms: tissue specificity and constitutive type. Among them, 9 genes, such as AetCCT3,AetCCT4,AetCCT7 and AetCCT9, were expressed in most tissues, while AetCCT15,AetCCT21 and AetCCT25 were expressed in seeds, respectively. The AetCCT family could respond to different exogenous hormones. After 24 hours and 72 hours of hormone application, all members showed the same response to the hormone, but different members had different responses to different hormones. The results indicated that the family members had some diversity in function and exercise mode, and might participate in different growth and development processes. Light condition affected the expression of AetCCT, indicating that the effect of light and vernalization is an important factor to affect and regulate the gene expression of the family. The results are helpful to explore the rules of evolution, acclimation and evolution of wheat (T.aestivum), and to understand the formation and interaction network of important agronomic traits.
【作者單位】： 山西省農(nóng)業(yè)科學(xué)院小麥研究所;山西省農(nóng)業(yè)科學(xué)院作物科學(xué)研究所作物遺傳與分子改良山西省重點(diǎn)實(shí)驗(yàn)室;中國(guó)科學(xué)院合肥物質(zhì)科學(xué)研究院技術(shù)生物與農(nóng)業(yè)工程研究所;
【基金】：國(guó)家自然科學(xué)基金青年基金(No.31601307) 山西省農(nóng)業(yè)攻關(guān)(No.20150311001-5) 山西省自然科學(xué)基金(No.2016011001) 山西省農(nóng)業(yè)科學(xué)院博士基金(No.YBSJJ1503)
【分類(lèi)號(hào)】：Q943.2

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