【摘要】：開花是植物生長發(fā)育的重要過程。CCT家族基因在植物中廣泛存在,參與植物花期的調控過程。該文從粗山羊草(Aegilops tauschii)全基因組中分離出26個CCT基因,它們分布于7對染色體上,按照排列順序將其命名為AetCCT1 26。AetCCT蛋白分子量介于14.9 kDa(AetCCT3) 83.2 kDa(AetCCT12)之間,其中有25個蛋白包含完整的CCT保守結構域。系統(tǒng)發(fā)育分析顯示,12對粗山羊草/烏拉爾圖小麥(Triticum urartu)CCT蛋白和9對粗山羊草/水稻(Oryza sativa)CCT蛋白為直系同源蛋白。通過公共數(shù)據(jù)的數(shù)字表達分析表明,AetCCT具有組織特異性和組成型2種表達形式,其中AetCCT3、AetCCT4、AetCCT7及AetCCT9等9個基因在大部分組織中都有表達,而AetCCT15、AetCCT21和AetCCT25等基因分別在種子、葉和根等少數(shù)組織中特異表達。AetCCT家族可以響應不同外源激素,施用激素24小時和72小時后各成員對激素響應整體表現(xiàn)一致,但不同成員對于不同激素的響應存在差異,表明該家族成員在功能和行使方式等方面具有一定的多樣性,可能參與不同生長發(fā)育過程。光照條件影響AetCCT的表達,說明光照和春化作用是影響與調控該家族基因表達的重要因素。研究結果有助于探索小麥(T.aestivum)進化、馴化和演變的規(guī)律,以及認識重要農藝性狀的形成與互作網絡。
[Abstract]:Flowering is an important process of plant growth and development. CCT family genes exist widely in plants and participate in the regulation of plant flowering. In this paper, 26 CCT genes were isolated from the whole (Aegilops tauschii) genome of Leymus chinensis. They were distributed on 7 pairs of chromosomes. They were named AetCCT1 26.AetCCT protein with molecular weight between 14.9 kDa (AetCCT3) and 83.2 kDa (AetCCT12). Among them, 25 proteins contain complete conserved domain of CCT. Phylogenetic analysis showed that 12 pairs of (Triticum urartu) CCT protein and 9 pairs of (Oryza sativa) CCT protein of Leymus chinensis / Ulaertu were direct homologous proteins. The results of digital expression analysis of common data showed that AetCCT had two expression forms: tissue specificity and constitutive type. Among them, 9 genes, such as AetCCT3,AetCCT4,AetCCT7 and AetCCT9, were expressed in most tissues, while AetCCT15,AetCCT21 and AetCCT25 were expressed in seeds, respectively. The AetCCT family could respond to different exogenous hormones. After 24 hours and 72 hours of hormone application, all members showed the same response to the hormone, but different members had different responses to different hormones. The results indicated that the family members had some diversity in function and exercise mode, and might participate in different growth and development processes. Light condition affected the expression of AetCCT, indicating that the effect of light and vernalization is an important factor to affect and regulate the gene expression of the family. The results are helpful to explore the rules of evolution, acclimation and evolution of wheat (T.aestivum), and to understand the formation and interaction network of important agronomic traits.
【作者單位】： 山西省農業(yè)科學院小麥研究所;山西省農業(yè)科學院作物科學研究所作物遺傳與分子改良山西省重點實驗室;中國科學院合肥物質科學研究院技術生物與農業(yè)工程研究所;
【基金】：國家自然科學基金青年基金(No.31601307) 山西省農業(yè)攻關(No.20150311001-5) 山西省自然科學基金(No.2016011001) 山西省農業(yè)科學院博士基金(No.YBSJJ1503)
【分類號】：Q943.2

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