干擾素刺激基因在雞傳染性支氣管炎病毒感染雛雞氣管黏膜中表達的動態(tài)變化
發(fā)布時間：2018-10-10 20:17
【摘要】：為了解雞干擾素刺激基因(ISG)在雞傳染性支氣管炎病毒(IBV)感染雛雞氣管黏膜中的表達情況,采用實時熒光定量PCR檢測IBV M41株感染SPF雞后不同時相氣管黏膜中β干擾素(IFN-β)和干擾素刺激基因mRNA轉(zhuǎn)錄水平和IBV病毒載量變化。結(jié)果顯示,雞氣管黏膜中IFN-β和干擾素刺激基因Mx、PKR、OAS、Viperin、IFITM3和IFIT5mRNA轉(zhuǎn)錄水平在感染后(DPI)第1~14天顯著(P0.05)或極顯著(P0.01)上調(diào),且均在5DPI達到峰值;雞氣管黏膜中IBV病毒載量在1DPI急劇升高,在5DPI達到峰值,14DPI病毒載量急劇下降。結(jié)果表明,IFN-β、干擾素刺激基因Mx、PKR、OAS、Viperin、IFITM3和IFIT5mRNA轉(zhuǎn)錄水平與IBV病毒載量變化趨勢一致,提示IFN-β、干擾素刺激基因Mx、PKR、OAS、Viperin、IFITM3和IFIT5在抵御IBV入侵、抑制IBV復制、增殖以及病毒清除中發(fā)揮重要作用。試驗結(jié)果豐富了宿主抗IBV感染的固有免疫應(yīng)答機制,為IBV感染的預防和控制提供了理論依據(jù)。
[Abstract]:To investigate the expression of chicken interferon stimulating gene (ISG) in tracheal mucosa of chickens infected with avian infectious bronchitis virus (IBV). The levels of interferon 尾 (IFN- 尾), IFN- stimulated gene mRNA transcription and IBV viral load in tracheal mucosa of IBV M41 strain infected with SPF chicken were detected by real-time fluorescence quantitative PCR. The results showed that the transcription levels of IFN- 尾 and interferon stimulating genes Mx,PKR,OAS,Viperin,IFITM3 and IFIT5mRNA in tracheal mucosa of chicken were significantly up-regulated (P0.05) or extremely significant (P0.01) on the 1st day after (DPI) infection, and both reached the peak in 5DPI, the viral load of IBV in chicken tracheal mucosa increased sharply in 1DPI. At the peak of 5DPI, the viral load of 14DPI decreased sharply. The results showed that the transcriptional levels of IFN- 尾, IFN- stimulated genes Mx,PKR,OAS,Viperin,IFITM3 and IFIT5mRNA were consistent with the changes of IBV viral load, suggesting that IFN- 尾, IFN- stimulated genes Mx,PKR,OAS,Viperin,IFITM3 and IFIT5 play an important role in resisting IBV invasion, inhibiting IBV replication, proliferation and virus clearance. The results enrich the innate immune response mechanism of host against IBV infection and provide a theoretical basis for the prevention and control of IBV infection.
【作者單位】： 廣西大學動物科學技術(shù)學院;
【基金】：國家自然科學基金項目(31360611) 廣西自然科學基金項目(2014GXNSFDA118011,2013GXNSFCA019010)
【分類號】：S858.31
本文編號：2263108
[Abstract]:To investigate the expression of chicken interferon stimulating gene (ISG) in tracheal mucosa of chickens infected with avian infectious bronchitis virus (IBV). The levels of interferon 尾 (IFN- 尾), IFN- stimulated gene mRNA transcription and IBV viral load in tracheal mucosa of IBV M41 strain infected with SPF chicken were detected by real-time fluorescence quantitative PCR. The results showed that the transcription levels of IFN- 尾 and interferon stimulating genes Mx,PKR,OAS,Viperin,IFITM3 and IFIT5mRNA in tracheal mucosa of chicken were significantly up-regulated (P0.05) or extremely significant (P0.01) on the 1st day after (DPI) infection, and both reached the peak in 5DPI, the viral load of IBV in chicken tracheal mucosa increased sharply in 1DPI. At the peak of 5DPI, the viral load of 14DPI decreased sharply. The results showed that the transcriptional levels of IFN- 尾, IFN- stimulated genes Mx,PKR,OAS,Viperin,IFITM3 and IFIT5mRNA were consistent with the changes of IBV viral load, suggesting that IFN- 尾, IFN- stimulated genes Mx,PKR,OAS,Viperin,IFITM3 and IFIT5 play an important role in resisting IBV invasion, inhibiting IBV replication, proliferation and virus clearance. The results enrich the innate immune response mechanism of host against IBV infection and provide a theoretical basis for the prevention and control of IBV infection.
【作者單位】： 廣西大學動物科學技術(shù)學院;
【基金】：國家自然科學基金項目(31360611) 廣西自然科學基金項目(2014GXNSFDA118011,2013GXNSFCA019010)
【分類號】：S858.31
【相似文獻】
相關(guān)博士學位論文 前1條
1 孫裴;雞氣管黏膜上皮細胞共培養(yǎng)體系的建立及IBV致細胞病變作用的研究[D];西北農(nóng)林科技大學;2008年
相關(guān)碩士學位論文 前1條
1 鄭衍生;IBV分離株GX-YL5感染對雞氣管黏膜和哈德氏腺免疫功能影響的研究[D];廣西大學;2013年
，本文編號：2263108
本文鏈接：http://sikaile.net/kejilunwen/jiyingongcheng/2263108.html
最近更新
教材專著
