雄激素受體基因第一外顯子CAG重復(fù)序列多態(tài)性與男性代謝綜合征及其組分的相關(guān)性
發(fā)布時(shí)間：2018-08-27 18:17
【摘要】：目的本研究從分子水平和遺傳學(xué)角度,探討寧夏地區(qū)回漢族人群AR基因CAG重復(fù)序列多態(tài)性與代謝綜合征基因遺傳性的關(guān)系,同時(shí)進(jìn)一步探討AR基因CAG重復(fù)序列多態(tài)性與代謝綜合征各組分及相應(yīng)疾病的相關(guān)性。方法本研究以整群抽樣的方法對(duì)寧夏地區(qū)銀川市與吳忠市社區(qū)居民進(jìn)行選擇,最終依據(jù)入組標(biāo)準(zhǔn)共納入研究對(duì)象953名。測(cè)量所有研究對(duì)象的身高、體重和血壓,檢測(cè)各項(xiàng)生化指標(biāo),包括高密度脂蛋白(HDL-C)、總膽固醇(TC)、甘油三酯(TG)、低密底脂蛋白(LDL-C)、空腹血糖(FBG)、空腹胰島素(FINS)以及各種性激素水平,運(yùn)用公式分別計(jì)算體質(zhì)量指數(shù)(BMI)、游離睪酮(FT)、胰島素抵抗指數(shù)(HOMA-IR)、胰島素敏感指數(shù)(HOMA-IS)和胰島β細(xì)胞功能指數(shù)(HOMA-β),基于毛細(xì)管電泳法原理,通過3730XL測(cè)序儀檢測(cè)目的片段AR基因CAG重復(fù)序列長(zhǎng)度,使用Gene Mapper4.1軟件識(shí)別測(cè)序結(jié)果。采用SPSS 23.0軟件研究對(duì)象的的一般人口學(xué)和臨床資料進(jìn)行描述,并對(duì)AR基因CAG重復(fù)序列多態(tài)性與代謝綜合征基因遺傳性及其組成成分的相關(guān)性進(jìn)行統(tǒng)計(jì)分析。結(jié)果1研究對(duì)象的一般情況1.1參照MS的診斷標(biāo)準(zhǔn),MS患者共317例,占全部研究對(duì)象的33.26%;NMS共636例,其中無代謝紊亂人群107例(11.23%),有一種代謝紊亂人群247例(25.92%),有兩種代謝紊亂人群282例(29.59%)。1.2與NMS組相比較,MS組的雌二醇(E2)、收縮壓(SBP)、舒張壓(DBP)、BMI、FBG、HOMA-IR、TG、TC指標(biāo)增加,總睪酮(TT)、E2/TT、HDL-C、HOMA-IS和HOMA-β均減低。1.3伴隨代謝紊亂組分的增加,SBP、DBP、BMI、FBG、HOMA-IR、TG和TC指標(biāo)增加,HDL-C指標(biāo)和HOMA-IS減低。2 AR基因第一外顯子CAG重復(fù)多態(tài)性的檢測(cè)結(jié)果及頻率分布情況2.1所有研究對(duì)象CAG重復(fù)多態(tài)性的重復(fù)數(shù)范圍是從10到35,平均長(zhǎng)度為22.85±2.90,出現(xiàn)頻率最高的是22。2.2 MS患者(317例)CAG重復(fù)數(shù)目最短重復(fù)數(shù)為14,最長(zhǎng)為35,平均長(zhǎng)度為22.78±2.96;代謝綜合征患者(636例)CAG重復(fù)數(shù)目最短重復(fù)數(shù)為10,最長(zhǎng)為35,平均長(zhǎng)度為22.88±2.87;無代謝紊亂人群(107例)CAG重復(fù)數(shù)目最短重復(fù)數(shù)為10,最長(zhǎng)為35,平均長(zhǎng)度為22.95±3.31;一種代謝紊亂人群(147例)CAG重復(fù)數(shù)目最短重復(fù)數(shù)為15,最長(zhǎng)為30,平均長(zhǎng)度為22.90±2.85;兩種代謝紊亂人群(282例)CAG重復(fù)數(shù)目最短重復(fù)數(shù)為10,最長(zhǎng)為35,平均長(zhǎng)度為22.84±2.71。以上各組出現(xiàn)頻率最高的均是22。2.3 MS組、NMS組、無代謝紊亂組、一種代謝紊亂組和兩種代謝紊亂組各組之間兩兩比較,AR基因CAG重復(fù)數(shù)目未見明顯差異。3 AR基因第一外顯子CAG重復(fù)多態(tài)性與MS基因遺傳性的關(guān)系3.1短組(CAG重復(fù)數(shù)22)中MS患者為99例(36.3%),長(zhǎng)組(CAG重復(fù)數(shù)≥22)中MS患者為218例(32.1%),AR基因CAG重復(fù)多態(tài)性對(duì)MS患病率的無影響。3.2短組(CAG重復(fù)數(shù)22)中無代謝紊亂共21例(7.7%),一種代謝紊亂共70例(25.6%),兩種代謝紊亂共83例(30.4%);長(zhǎng)組(CAG重復(fù)數(shù)≥22)中無代謝紊亂共86例(12.6%),一種代謝紊亂共177例(26.0%),兩種代謝紊亂共199例(29.3%)。短組和長(zhǎng)組間代謝紊亂組成成分的構(gòu)成比無差異。4 AR基因第一外顯子上CAG重復(fù)多態(tài)性與性激素水平、MS組分的相關(guān)性4.1 A R基因CAG重復(fù)次數(shù)與各性激素水平均不具有相關(guān)性;AR基因CAG重復(fù)數(shù)長(zhǎng)組和短組之間各激素水平的均無差異。4.2 A R基因CAG重復(fù)次數(shù)與SBP和HDL-C顯著相關(guān),CAG重復(fù)次數(shù)與SBP呈負(fù)相關(guān),此相關(guān)性獨(dú)立于年齡、BMI、TT和FBG對(duì)SBP的影響之外;AR基因CAG重復(fù)次數(shù)與血清HDL-C水平呈顯著正相關(guān),此相關(guān)性獨(dú)立于BMI、TG和TC對(duì)HDL-C的影響之外。AR基因CAG重復(fù)次數(shù)與DBP、TC、TG、LDL-C、FBG、FINS、HOMA-IR、HOMA-IS和HOMA-β均無相關(guān)性。4.3 C AG重復(fù)數(shù)22組的SBP明顯高于CAG重復(fù)數(shù)≥22組,而CAG重復(fù)數(shù)22組HDL-C顯著低CAG重復(fù)數(shù)≥22長(zhǎng)組。而兩組之間DBP、TC、TG、LDL-C、FBG、FINS、HOMA-IR、HOMA-IS和HOMA-β均無差異。4.4短組(CAG重復(fù)數(shù)22)中高血壓患者為194例(71.1%),長(zhǎng)組(CAG重復(fù)數(shù)≥22)中高血壓患者為414例(60.9%),CAG重復(fù)數(shù)22組有更高的高血壓患病率;經(jīng)多因素Logistic回歸分析,AR基因CAG重復(fù)數(shù)為高血壓發(fā)生的影響因素,且AR基因CAG重復(fù)數(shù)22的人發(fā)生高血壓的危險(xiǎn)度是AR基因CAG重復(fù)數(shù)≥22的人的1.4倍。CAG重復(fù)數(shù)長(zhǎng)短組對(duì)HDL-C或TG異常、超重及(或)肥胖、糖尿病以及高血糖的發(fā)生率均無影響。結(jié)論AR基因CAG重復(fù)多態(tài)性在無代謝紊亂組、一種代謝紊亂組、兩種代謝紊亂組、MS組和NMS組的頻率分布情況基本一致,AR基因CAG重復(fù)多態(tài)性與MS的基因遺傳性無相關(guān)性;AR基因CAG重復(fù)序列長(zhǎng)度與MS各組分的相關(guān)分析顯示,AR基因CAG重復(fù)序列長(zhǎng)度與HDL-C水平呈正相關(guān),而與SBP水平呈負(fù)相關(guān),校正年齡影響因素后,其相關(guān)性仍然存在;AR基因CAG重復(fù)長(zhǎng)度不同分組間,HDL-C、SBP水平存在差異,CAG重復(fù)數(shù)22會(huì)引起HDL-C水平的降低和SBP水平的升高;AR基因CAG重復(fù)序列長(zhǎng)度與MS各組分異常相應(yīng)疾病的相關(guān)分析顯示,AR基因CAG重復(fù)數(shù)22組高血壓的患病率明顯高于CAG重復(fù)≥22組,AR基因CAG重復(fù)數(shù)22的人發(fā)生高血壓的危險(xiǎn)度是AR基因CAG重復(fù)數(shù)≥22的人的1.4倍。
[Abstract]:Objective To explore the relationship between the CAG repeat polymorphism of AR gene and the inheritance of metabolic syndrome gene in Hui and Han population in Ningxia from the molecular level and genetics, and to explore the relationship between the CAG repeat polymorphism of AR gene and the components of metabolic syndrome and the corresponding diseases. Methods 953 residents in Yinchuan and Wuzhong communities in Ningxia were selected and enrolled in the study according to the enrollment criteria. Height, weight and blood pressure were measured, and biochemical parameters including high density lipoprotein (HDL-C), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-C), fasting blood glucose (FBG) were measured. Body mass index (BMI), free testosterone (FT), insulin resistance index (HOMA-IR), insulin sensitivity index (HOMA-IS) and islet beta cell function index (HOMA-beta) were calculated by using the formula. Based on the principle of capillary electrophoresis, the target fragment of AR gene CAG was detected by 3730XL sequencer. The length of repeat sequence was identified by Gene Mapper 4.1 software. The general demographic and clinical data of the subjects were described by SPSS 23.0 software. The correlation between the CAG repeat polymorphism of AR gene and the inheritance of metabolic syndrome gene and its components was statistically analyzed. Status 1.1 According to the diagnostic criteria of MS, there were 317 patients with MS, accounting for 33.26% of all subjects; 636 patients with NMS, including 107 patients without metabolic disorders (11.23%), 247 patients with one metabolic disorder (25.92%) and 282 patients with two metabolic disorders (29.59%). Increased HOMA-IR, TG, TC, total testosterone (TT), E2/TT, HDL-C, HOMA-IS and HOMA-beta were all decreased.1.3 With the increase of metabolic disorder components, increased SBP, DBP, BMI, FBG, HOMA-IR, TG and TC, decreased HDL-C and HOMA-IS. 2.1 CAG repeat polymorphism and frequency distribution of the first exon of AR gene were detected in all subjects. The average length of CAG repeats ranged from 10 to 35, with the highest frequency of 22.2.2 MS (317 cases) and the shortest, longest and average length of CAG repeats ranged from 14 to 35. The shortest, longest and average length of CAG repeats ranged from 10 to 35 in patients with metabolic syndrome (636 cases). The shortest number of CAG repeats was 10, the longest was 35, and the average length was 22.95 (+ 3.31); the shortest number of CAG repeats was 15, the longest was 30, and the average length was 22.90 (+ 2.85) in one metabolic disorder group (147); the shortest number of CAG repeats was 10, the longest was 35, and the average length was 32.90 (+ 2.85) in two metabolic disorders groups (282). The highest frequency was 22.2.3 MS group, NMS group, no metabolic disorder group, one metabolic disorder group and two metabolic disorder groups. There was no significant difference in the number of CAG repeats in AR gene. 3 The relationship between CAG repeat polymorphism in the first exon of AR gene and the inheritance of MS gene was 3.1 short group (CAG repeat). Among the 22 patients, 99 (36.3%) had MS, and 218 (32.1%) had MS in the long group (CAG repeats (> 22). There was no effect of CAG repeat polymorphism on the prevalence of MS. 21 (7.7%) had no metabolic disorder in the short group (CAG repeats 22), 70 (25.6%) had one metabolic disorder, 83 (30.4%) had two metabolic disorders, and no generation in the long group (CAG repeats (> 22). There were 86 cases (12.6%) with metabolic disorders, 177 cases (26.0%) with one metabolic disorder and 199 cases (29.3%) with two metabolic disorders. The CAG repeats of AR gene were significantly correlated with SBP and HDL-C. The CAG repeats of AR gene were negatively correlated with SBP, independent of age, BMI, TT and FBG. The CAG repeats of AR gene were positively correlated with serum HDL-C levels. The correlation was independent of the effects of BMI, TG and TC on HDL-C. The number of CAG repeats of AR gene was not correlated with DBP, TC, TG, LDL-C, FBG, FINS, HOMA-IR, HOMA-IS and HOMA-beta. The number of SBP in 22 groups of HOMA-IS repeats was significantly higher than that in 22 groups of CAG repeats, while the number of HDL-C repeats in 22 groups of CAG repeats was significantly lower than that in 22 groups of CAG repeats. There was no significant difference among - C, FBG, FINS, HOMA - IR, HOMA - IS and HOMA - beta. Among the short group (CAG repeats 22), 194 (71.1%) were hypertensive, 414 (60.9%) were hypertensive in the long group (CAG repeats more than 22), and 22 (CAG repeats more than 22) had a higher prevalence of hypertension. The risk of hypertension was 1.4 times higher in patients with CAG repeat 22 of AR gene than in those with CAG repeat 22 of AR gene. The length of CAG repeat had no effect on the incidence of HDL-C or TG abnormalities, overweight and/or obesity, diabetes mellitus and hyperglycemia. There was no correlation between the CAG repeat polymorphism of AR gene and the genetic inheritance of MS. The correlation analysis between the CAG repeat length of AR gene and the components of MS showed that the CAG repeat length of AR gene was positively correlated with HDL-C level, but negatively correlated with SBP level. The correlation between the CAG repeat length of AR gene and the disease related to the abnormal components of MS showed that the prevalence of hypertension was significant in 22 groups of AR gene CAG repeat length. The risk of hypertension was 1.4 times higher in group 22 than in group 22.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R589
本文編號(hào)：2208055
[Abstract]:Objective To explore the relationship between the CAG repeat polymorphism of AR gene and the inheritance of metabolic syndrome gene in Hui and Han population in Ningxia from the molecular level and genetics, and to explore the relationship between the CAG repeat polymorphism of AR gene and the components of metabolic syndrome and the corresponding diseases. Methods 953 residents in Yinchuan and Wuzhong communities in Ningxia were selected and enrolled in the study according to the enrollment criteria. Height, weight and blood pressure were measured, and biochemical parameters including high density lipoprotein (HDL-C), total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL-C), fasting blood glucose (FBG) were measured. Body mass index (BMI), free testosterone (FT), insulin resistance index (HOMA-IR), insulin sensitivity index (HOMA-IS) and islet beta cell function index (HOMA-beta) were calculated by using the formula. Based on the principle of capillary electrophoresis, the target fragment of AR gene CAG was detected by 3730XL sequencer. The length of repeat sequence was identified by Gene Mapper 4.1 software. The general demographic and clinical data of the subjects were described by SPSS 23.0 software. The correlation between the CAG repeat polymorphism of AR gene and the inheritance of metabolic syndrome gene and its components was statistically analyzed. Status 1.1 According to the diagnostic criteria of MS, there were 317 patients with MS, accounting for 33.26% of all subjects; 636 patients with NMS, including 107 patients without metabolic disorders (11.23%), 247 patients with one metabolic disorder (25.92%) and 282 patients with two metabolic disorders (29.59%). Increased HOMA-IR, TG, TC, total testosterone (TT), E2/TT, HDL-C, HOMA-IS and HOMA-beta were all decreased.1.3 With the increase of metabolic disorder components, increased SBP, DBP, BMI, FBG, HOMA-IR, TG and TC, decreased HDL-C and HOMA-IS. 2.1 CAG repeat polymorphism and frequency distribution of the first exon of AR gene were detected in all subjects. The average length of CAG repeats ranged from 10 to 35, with the highest frequency of 22.2.2 MS (317 cases) and the shortest, longest and average length of CAG repeats ranged from 14 to 35. The shortest, longest and average length of CAG repeats ranged from 10 to 35 in patients with metabolic syndrome (636 cases). The shortest number of CAG repeats was 10, the longest was 35, and the average length was 22.95 (+ 3.31); the shortest number of CAG repeats was 15, the longest was 30, and the average length was 22.90 (+ 2.85) in one metabolic disorder group (147); the shortest number of CAG repeats was 10, the longest was 35, and the average length was 32.90 (+ 2.85) in two metabolic disorders groups (282). The highest frequency was 22.2.3 MS group, NMS group, no metabolic disorder group, one metabolic disorder group and two metabolic disorder groups. There was no significant difference in the number of CAG repeats in AR gene. 3 The relationship between CAG repeat polymorphism in the first exon of AR gene and the inheritance of MS gene was 3.1 short group (CAG repeat). Among the 22 patients, 99 (36.3%) had MS, and 218 (32.1%) had MS in the long group (CAG repeats (> 22). There was no effect of CAG repeat polymorphism on the prevalence of MS. 21 (7.7%) had no metabolic disorder in the short group (CAG repeats 22), 70 (25.6%) had one metabolic disorder, 83 (30.4%) had two metabolic disorders, and no generation in the long group (CAG repeats (> 22). There were 86 cases (12.6%) with metabolic disorders, 177 cases (26.0%) with one metabolic disorder and 199 cases (29.3%) with two metabolic disorders. The CAG repeats of AR gene were significantly correlated with SBP and HDL-C. The CAG repeats of AR gene were negatively correlated with SBP, independent of age, BMI, TT and FBG. The CAG repeats of AR gene were positively correlated with serum HDL-C levels. The correlation was independent of the effects of BMI, TG and TC on HDL-C. The number of CAG repeats of AR gene was not correlated with DBP, TC, TG, LDL-C, FBG, FINS, HOMA-IR, HOMA-IS and HOMA-beta. The number of SBP in 22 groups of HOMA-IS repeats was significantly higher than that in 22 groups of CAG repeats, while the number of HDL-C repeats in 22 groups of CAG repeats was significantly lower than that in 22 groups of CAG repeats. There was no significant difference among - C, FBG, FINS, HOMA - IR, HOMA - IS and HOMA - beta. Among the short group (CAG repeats 22), 194 (71.1%) were hypertensive, 414 (60.9%) were hypertensive in the long group (CAG repeats more than 22), and 22 (CAG repeats more than 22) had a higher prevalence of hypertension. The risk of hypertension was 1.4 times higher in patients with CAG repeat 22 of AR gene than in those with CAG repeat 22 of AR gene. The length of CAG repeat had no effect on the incidence of HDL-C or TG abnormalities, overweight and/or obesity, diabetes mellitus and hyperglycemia. There was no correlation between the CAG repeat polymorphism of AR gene and the genetic inheritance of MS. The correlation analysis between the CAG repeat length of AR gene and the components of MS showed that the CAG repeat length of AR gene was positively correlated with HDL-C level, but negatively correlated with SBP level. The correlation between the CAG repeat length of AR gene and the disease related to the abnormal components of MS showed that the prevalence of hypertension was significant in 22 groups of AR gene CAG repeat length. The risk of hypertension was 1.4 times higher in group 22 than in group 22.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R589
【參考文獻(xiàn)】
中國(guó)期刊全文數(shù)據(jù)庫(kù) 前7條
1 Qun Zhang;Xiaofeng Zhang;Mengmeng Zhao;Hanpeng Huang;Ning Ding;Xilong Zhang;Hong Wang;;Correlation of obstructive sleep apnea hypopnea syndrome with metabolic syndrome in snorers[J];The Journal of Biomedical Research;2014年03期
2 毛達(dá)勇;趙娟;張吉才;;2型糖尿病并發(fā)血管疾病男性患者雄激素受體基因(CAG)n多態(tài)性研究[J];國(guó)際檢驗(yàn)醫(yī)學(xué)雜志;2012年12期
3 郭恒;馬儒林;張景玉;芮東升;徐上知;孫鳳;郭淑霞;;新疆哈薩克族與漢族代謝綜合征流行特點(diǎn)比較與分析[J];中華高血壓雜志;2011年06期
4 劉貴秋;李剛;鞏麗;張偉;馮英明;蘇勤;;雄激素受體基因第一外顯子CAG重復(fù)序列長(zhǎng)度多態(tài)性與肺癌的關(guān)系[J];現(xiàn)代腫瘤醫(yī)學(xué);2006年08期
5 劉貴秋,蘇勤,張偉,刁小莉,馮英明;雄激素受體基因第一外顯子CAG重復(fù)序列長(zhǎng)度多態(tài)性與男性膀胱癌的發(fā)生有關(guān)[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2005年21期
6 戴繼燦,李躍輝,江魚;雄激素水平增齡變化對(duì)老年男性的影響[J];中華男科學(xué);2001年01期
7 李長(zhǎng)嶺,董金堂,Tavis Sipe,呂寧,萬儀增,Henry F.Frierson Jr,Leland W.K.Chung;雄激素受體基因微衛(wèi)星多態(tài)性與前列腺癌的生物學(xué)行為[J];實(shí)用癌癥雜志;2001年01期
中國(guó)重要會(huì)議論文全文數(shù)據(jù)庫(kù) 前1條
1 沈琳輝;林云;顏美珠;趙雅潔;趙詠桔;;老年男性雄激素受體基因CAG重復(fù)序列長(zhǎng)度多態(tài)性與代謝綜合征的關(guān)系[A];中華醫(yī)學(xué)會(huì)第八次全國(guó)老年醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2007年
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