【摘要】：【目的】本研究旨在探究銼吸式口器害蟲西花薊馬Frankliniella occidentalis取食誘導的菜豆Phaseolus vulgaris木植株系統(tǒng)防御反應的分子機制�！痉椒ā坷脽晒舛縋CR技術(shù),檢測西花薊馬分別在穩(wěn)定取食菜豆植株2 h后的24,48,72和96 h菜豆植株不同部位(上部、中部和下部)葉片中茉莉酸信號轉(zhuǎn)導途徑和水楊酸信號轉(zhuǎn)導途徑防御相關(guān)基因脂氧合酶基因(LOX)、苯丙氨酸解氨酶基因(PAL)和β-1,3-葡聚糖酶基因(PR-2)相對表達量的變化�！窘Y(jié)果】西花薊馬取食菜豆植株后,受害葉片及上部和下部健康葉片中LOX,PAL和PR-2均被顯著誘導表達,均以受害葉片防御酶基因表達量的變化較大,并且表達量在相同葉片不同時間下的變化不同。中部受害葉片中LOX的相對表達量均顯著高于未接蟲的對照植株,并隨西花薊馬為害時間的延長逐漸升高,在96 h時為對照的209.54倍;隨著時間的延長LOX表達量在上部和下部健康葉片呈現(xiàn)升高-降低-升高的趨勢。PAL的表達量在西花薊馬為害植株的中部受害葉片和上部健康葉片均于48 h時出現(xiàn)最大值,分別為對照的52.70倍和41.20倍;但在下部健康葉片于72 h時達到最大值,為對照的47.06倍。PR-2表達量在受害株的上部健康葉片于48 h時出現(xiàn)最大值,在中部受害葉片和下部健康葉片均于96 h時達到最大值,但在24 h時在下部健康葉片中受到抑制�！窘Y(jié)論】西花薊馬取食能顯著誘導菜豆植株茉莉酸和水楊酸信號轉(zhuǎn)導途徑相關(guān)防御酶基因LOX,PAL和PR-2的表達,并在菜豆植株不同部位葉片產(chǎn)生系統(tǒng)抗性。
[Abstract]:[objective] to explore the molecular mechanism of systemic defense response induced by Frankliniella occidentalis, a rasping mouthpiece pest, in Phaseolus vulgaris plantlets. [methods] fluorescence quantitative PCR technique was used. The different parts (upper part) of vegetable bean plant were detected at 244872 and 96 h after stable feeding for 2 h. Changes in the relative expression levels of Jasmonic Acid signal Transduction Pathway and Salicylic Acid signal Transduction Pathway Defense related Gene Lipoxygene-associated Gene (LOX), phenylalanine Ammonia-lyase Gene (PAL) and 尾 -1 3-glucanase Gene (PR-2) in the Middle and Lower part of the leaves. [ Results: after feeding on bean plants, thrips of western flowers, The expression of LOXPAL and PR-2 in the injured leaves and the upper and lower healthy leaves were significantly induced. The expression of defense enzyme genes in the injured leaves varied greatly, and the expression levels varied at different time points in the same leaves. The relative expression of LOX in the middle damaged leaves was significantly higher than that in the control plants without inoculation, and increased gradually with the prolongation of the damage time of the western flower thrips, and was 209.54 times of that of the control at 96 h. With the extension of time, the expression of LOX increased, decreased and increased in the upper and lower healthy leaves. The expression of pal reached the maximum at 48 h in the middle injured leaves and the upper healthy leaves of the plants damaged by thrips sibirica. The expression of PR-2 was 52.70 times and 41.20 times of the control, respectively, but reached the maximum at 72 h in the lower healthy leaves, and the maximum expression of PR-2 was 47.06 times at 48 h in the upper healthy leaves of the affected plants. The maximum value of damaged leaves and lower healthy leaves was reached at 96 h. But it was inhibited in the lower part of the leaves at 24 h. [conclusion] the feeding of thrips can significantly induce the expression of LOXPAL and PR-2 genes related to the signal transduction pathway of jasmonic acid and salicylic acid in bean plants. Phylogenetic resistance was produced in different parts of soybean leaves.
【作者單位】： 貴州大學昆蟲研究所貴州省山地農(nóng)業(yè)病蟲害重點實驗室;
【基金】：國家自然科學基金項目(31160367) 貴州省農(nóng)業(yè)攻關(guān)項目(黔科合NY[2015]3014-1號)
【分類號】：S436.43

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