【摘要】：Hvsusiba2是一個(gè)來源于大麥的糖信號(hào)轉(zhuǎn)錄因子,它作用于淀粉合成途徑的上游,是大麥淀粉合成途徑的關(guān)鍵調(diào)控因子。本研究利用前期研究獲得的轉(zhuǎn)Hvsusiba2水稻純合株系,探討Hvsusiba2轉(zhuǎn)錄因子過表達(dá)對(duì)水稻淀粉合成的影響,并通過酶譜分析方法分析Hvsusiba2在水稻中的異源表達(dá)對(duì)支鏈淀粉合成同工酶的調(diào)控作用。結(jié)果顯示:Hvsusiba2在水稻中的異源表達(dá)能夠提高籽粒中總淀粉含量,增加淀粉含量達(dá)4.33%~9.22%,莖鞘的淀粉含量提高1倍,幼嫩種子發(fā)育過程中淀粉累積快速增加期為花后15d起。對(duì)該時(shí)期的支鏈淀粉合成酶同工酶分析提示異源表達(dá)Hvsusiba2是通過提高籽粒中淀粉分支酶中淀粉分支酶SBEIIb、淀粉分支酶SBEI、異淀粉酶Isoamylase以及淀粉酶Amylase的活性改變水稻的支鏈淀粉合成,但淀粉分支酶中SBEIIa的活性不受Hvsusiba2轉(zhuǎn)錄因子的調(diào)控。
[Abstract]:Hvsusiba 2 is a sugar signaling transcription factor from barley. It acts on the upstream of the starch synthesis pathway and is a key regulator of the starch synthesis pathway in barley. In this study, the effect of the overexpression of Hvsusiba 2 transcription factor on starch synthesis in rice was investigated by using the rice homozygous lines of Hvsusiba 2 obtained from previous studies. The results showed that the heterologous expression of Hvsusiba 2 in rice could increase the total starch content in grains, increase the starch content by 4.33%~9.22%, increase the starch content in stems and sheaths by 1 fold, and increase the starch accumulation rapidly during the development of young seeds. Isozyme analysis of amylopectin synthase suggested that heterologous expression of Hvsusiba 2 altered amylopectin synthesis in Rice by increasing the activities of SBEIIb, SBEI, Isoamylase and Amylase in amylopectin, but SBEIIa in amylopectin changed amylopectin synthesis. Activity is not regulated by Hvsusiba2 transcription factors.
【作者單位】： 福建省農(nóng)業(yè)遺傳工程重點(diǎn)實(shí)驗(yàn)室/福建省農(nóng)業(yè)科學(xué)院生物技術(shù)研究所;
【基金】：福建省科技計(jì)劃項(xiàng)目——省屬公益類科研院所基本科研專項(xiàng)(2015N0037) 福建省農(nóng)業(yè)科學(xué)院科技重大專項(xiàng)(A2015-01)
【分類號(hào)】：S511
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本文編號(hào)：2198174