【摘要】：為了從分子水平上探討優(yōu)質(zhì)小麥資源中LMW-GS等位基因與小麥品質(zhì)的關(guān)系,以及在改善小麥品質(zhì)方面的潛在價(jià)值,利用小麥Glu-A3和Glu-B3基因的特異引物從強(qiáng)筋型、中筋型和弱筋型小麥共計(jì)10份材料中分離出LMW-GS基因后進(jìn)行序列分析。結(jié)果表明,共發(fā)現(xiàn)14個(gè)新的核苷酸變異類型和4個(gè)肽鏈變異類型。其中,14個(gè)新的核苷酸變異類型中,4個(gè)為Glu-A3基因變異類型,1個(gè)為Glu-B3基因變異類型,9個(gè)為Glu-D3基因變異類型。值得注意的是,有2個(gè)半胱氨酸數(shù)目特殊的亞基類型被發(fā)現(xiàn),一個(gè)是來自師欒02-1含有9個(gè)半胱氨酸殘基的GluA3-18基因,另一個(gè)是來自偃展4110含有7個(gè)半胱氨酸殘基的GluD3-13基因。
[Abstract]:In order to explore the relationship between LMW-GS allele and wheat quality in wheat resources at molecular level and its potential value in improving wheat quality, the special primers of Glu-A3 and Glu-B3 genes were used to analyze the strong gluten type of wheat. LMW-GS gene was isolated from 10 middle gluten and weak gluten wheat and sequenced. The results showed that 14 new nucleotide variants and 4 peptide chain variants were found. Of the 14 new nucleotide variation types, 4 were Glu-A3 gene variants, 1 was Glu-B3 gene variants, and 9 were Glu-D3 gene variants. It is worth noting that there are two specific subunits of cysteine, one from the GluA3-18 gene containing 9 cysteine residues in Shi Luan 02-1, and the other from the GluD3-13 gene with seven cysteine residues in Yanchang 4110.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院植物生物技術(shù)功能實(shí)驗(yàn)室;河南省農(nóng)業(yè)科學(xué)院小麥研究所/河南省小麥生物學(xué)重點(diǎn)實(shí)驗(yàn)室/小麥國家工程實(shí)驗(yàn)室/農(nóng)業(yè)部黃淮中部小麥生物學(xué)與遺傳育種重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(31271726) 內(nèi)蒙古農(nóng)業(yè)大學(xué)優(yōu)秀青年科學(xué)基金項(xiàng)目(2014XYQ-18) 內(nèi)蒙古自治區(qū)自然科學(xué)基金項(xiàng)目(2014MS0338) 內(nèi)蒙古自治區(qū)產(chǎn)業(yè)創(chuàng)新創(chuàng)業(yè)人才團(tuán)隊(duì)項(xiàng)目(201503004)
【分類號】：S512.1

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