【摘要】：目的膀胱癌是泌尿系統(tǒng)最常見(jiàn)的惡性腫瘤之一,關(guān)于膀胱癌的發(fā)病機(jī)制仍未得到全面而深入的研究。LASS2是我國(guó)新近發(fā)現(xiàn)的腫瘤抑制基因,文中通過(guò)檢測(cè)LASS2在裸鼠膀胱癌模型中的表達(dá),探討LASS2與腫瘤增殖和凋亡的關(guān)系及可能的分子機(jī)制。方法采用抽簽方法將30只裸鼠隨機(jī)分為皮下種植組、原代灌注組及空白對(duì)照組(DMEM膀胱癌細(xì)胞培養(yǎng)基),每組10只。皮下膀胱癌種植模型將已制備的細(xì)胞懸液按每只0.5 mL(即5×10~6/0.1 mL個(gè)細(xì)胞)注射入裸鼠左腋皮下。原位膀胱癌種植模型在裸鼠排空膀胱后將100μL EJ(即1×10~7個(gè)細(xì)胞)單細(xì)胞懸液灌注入膀胱,觀察裸鼠致瘤情況。檢測(cè)不同部位成瘤組織中LASS2、Ki-67的表達(dá)及增殖、凋亡等指標(biāo)變化。結(jié)果皮下種植組皮下腫瘤形成,成瘤率為100%。原位灌注組、皮下種植組及空白對(duì)照組裸鼠肉眼及病理切片均未檢測(cè)到肝、肺、腎等器官及淋巴結(jié)轉(zhuǎn)移。與空白對(duì)照組LASS2表達(dá)(81.0%)比較,原位灌注組、皮下種植組LASS2表達(dá)(60.0%、14.0%)顯著減少(P0.05);與空白對(duì)照組Ki-67表達(dá)(16.0%)比較,原位灌注組、皮下種植組Ki-67表達(dá)(50.0%、78.0%)增加(P0.05);與原位灌注組比較,皮下種植組LASS2表達(dá)(14.0%)明顯減少(P0.05),Ki-67表達(dá)(78.0%)增加(P0.05)。與空白對(duì)照組比較,皮下種植組、原位灌注組Bcl-2表達(dá)明顯升高(P0.05);與皮下種植組比較,原位灌注組Bcl-2表達(dá)升高(P0.05);而B(niǎo)cl-xl在原位種植瘤組織中的表達(dá)高于其他兩組(P0.05);Bax和Bim均呈現(xiàn)低表達(dá),Bax在各組中的表達(dá)水平差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);與空白對(duì)照組比較,皮下種植組Bim表達(dá)明顯降低(P0.05),而原位種植瘤組差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。caspase3在各組組織中的表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);而ki-67在皮下種植瘤組織中的表達(dá)較其他兩種腫瘤組織中的表達(dá)顯著升高(P0.05);LASS2在空白對(duì)照組中的表達(dá)較其他兩組表達(dá)顯著升高(P0.05)。結(jié)論 LASS2具有抑制腫瘤增殖的作用,其表達(dá)可能與膀胱癌EJ細(xì)胞體內(nèi)成瘤能力、增殖和凋亡有關(guān)。
[Abstract]:Objective bladder cancer is one of the most common malignant tumors in the urinary system. The pathogenesis of bladder cancer has not been fully and thoroughly studied. LASS2 is a newly discovered tumor suppressor gene in China. By detecting the expression of LASS2 in nude mice bladder cancer model, the relationship between LASS2 and tumor proliferation and apoptosis and its possible molecular mechanism were studied. Methods by drawing lots, 30 nude mice were randomly divided into subcutaneous implantation group, primary perfusion group and blank control group (DMEM bladder cancer cell culture medium) with 10 in each group. The subcutaneous bladder cancer implantation model injected the prepared cell suspensions into the left axillary subcutaneous of nude mice in 0.5 mL (5 脳 10 ~ (6) / 0. 1 mL cells). In situ bladder cancer implantation model, 100 渭 L EJ (1 脳 107 cells) single cell suspension was perfused into the bladder after bladder emptying in nude mice, and the tumorigenesis of nude mice was observed. The expression, proliferation and apoptosis of LASS2 Ki-67 were detected in different tumor tissues. Results the subcutaneous tumor was formed in the subcutaneous implantation group, and the tumorigenesis rate was 100%. In situ perfusion group, subcutaneous implantation group and blank control group, liver, lung, kidney and lymph node metastasis were not detected in naked eyes and pathological sections of nude mice. Compared with the blank control group (81.0%), the expression of LASS2 in the subcutaneous implantation group (60.0%) decreased significantly (P0.05), and compared with the blank control group (16.0%), the expression of Ki-67 increased (50.0%) in the subcutaneous implantation group (P0.05), and increased in the subcutaneous implantation group (78.0%) (P0.05), compared with the control group (16.0%). The expression of LASS2 (14.0%) in subcutaneous implant group was significantly decreased (P0.05) and Ki-67 expression (78.0%) was increased (P0.05). Compared with the blank control group, the expression of Bcl-2 in the subcutaneous implantation group and in situ perfusion group was significantly increased (P0.05), and compared with the subcutaneous implantation group, The expression of Bcl-2 in situ perfusion group was higher than that in the other two groups (P0.05), and there was no significant difference in the expression of Bcl-xl between the two groups (P0.05). The expression of Bim in subcutaneous implantation group was significantly decreased (P0.05), but there was no significant difference in expression of caspase3 in subcutaneous implanted tumor group (P0.05), while the expression of ki-67 in subcutaneous implanted tumor tissue was higher than that in other two kinds of tumor tissues (P0.05). The expression of LASS2 in the blank control group was significantly higher than that in the other two groups (P0.05). Conclusion LASS2 can inhibit tumor proliferation, and its expression may be related to tumor formation, proliferation and apoptosis of bladder cancer EJ cells.
【作者單位】： 昆明醫(yī)科大學(xué)第二附屬醫(yī)院泌尿外科;
【基金】：國(guó)家自然科學(xué)基金(81460384) 云南省博士新人獎(jiǎng)項(xiàng)目(60116090706) 云南省教育廳科學(xué)研究基金(2014J045)
【分類號(hào)】：R-332;R737.14

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 譚景瑩;人膀胱癌致癌基因產(chǎn)物和線粒體合酶間的同源性[J];生理科學(xué);1983年05期

2 楊慎敏;溫端改;侯建全;何軍;岑建農(nóng);陳劍華;;原位膀胱癌動(dòng)物模型的建立及應(yīng)用[J];癌癥;2007年04期

3 郭俊杰;劉吉成;王麗萍;;青春雙歧桿菌細(xì)胞壁免疫調(diào)節(jié)作用及其對(duì)膀胱癌細(xì)胞抑制作用研究[J];醫(yī)學(xué)研究雜志;2008年01期

4 梁中錕;張琳;胡志明;陳忠;黃鑫;石向華;譚萬(wàn)龍;高基民;;新方法簡(jiǎn)便高效建立小鼠原位膀胱癌模型[J];南方醫(yī)科大學(xué)學(xué)報(bào);2009年04期

5 許文平;林宗明;張建平;侯佳舟;王國(guó)民;;叢生蛋白反義核苷酸對(duì)裸鼠人膀胱癌模型的腫瘤抑制作用[J];復(fù)旦學(xué)報(bào)(醫(yī)學(xué)版);2008年05期

6 吳強(qiáng),楚雍烈,任會(huì)勛,王建安;p16/CDKN2對(duì)膀胱癌細(xì)胞生長(zhǎng)的抑制[J];癌癥;1997年05期

7 任明;梁新;劉祿成;李然偉;郭航;王頌;;荷瘤裸鼠膀胱癌原位模型的建立[J];中國(guó)老年學(xué)雜志;2007年09期

8 顏汝平;王劍松;徐鴻毅;;膀胱癌原位移植瘤動(dòng)物模型[J];云南醫(yī)藥;2006年04期

9 楊芳莉;赫蘭;于淼;曹妍;祁贊梅;劉北星;劉軍;呂昌龍;;分枝桿菌Ag85A DNA疫苗對(duì)膀胱癌小鼠細(xì)胞免疫功能的影響[J];微生物學(xué)雜志;2009年06期

10 侯樹(shù)坤,朱積川,張小東,李鷹,王曉峰;E-cadherin基因突變對(duì)膀胱癌侵襲能力影響的研究[J];中華泌尿外科雜志;1999年05期

相關(guān)博士學(xué)位論文 前2條

1 施挺;膀胱癌5637細(xì)胞系的體外培養(yǎng)及荷瘤裸鼠實(shí)驗(yàn)動(dòng)物模型的建立[D];北京協(xié)和醫(yī)學(xué)院;2012年

2 金百冶;基于蛋白質(zhì)組學(xué)技術(shù)篩選膀胱癌相關(guān)蛋白的研究[D];浙江大學(xué);2013年

，

本文編號(hào)：2180438